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[1]梁玲,方豆豆.腫瘤微環(huán)境中黑磷納米片對(duì)腺苷激酶的調(diào)控研究[J].福建醫(yī)藥雜志,2025,47(02):51-55.[doi:10.20148/j.fmj.2025.02.014]
 LIANG Ling,FANG Doudou.Regulation of black phosphorus nanosheets on adenosine kinase in tumor microenvironment[J].FUJIAN MEDICAL JOURNAL,2025,47(02):51-55.[doi:10.20148/j.fmj.2025.02.014]
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腫瘤微環(huán)境中黑磷納米片對(duì)腺苷激酶的調(diào)控研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
47
期數(shù):
2025年02期
頁碼:
51-55
欄目:
基礎(chǔ)研究
出版日期:
2025-02-20

文章信息/Info

Title:
Regulation of black phosphorus nanosheets on adenosine kinase in tumor microenvironment
文章編號(hào):
1002-2600(2025)02-0051-05
作者:
梁玲方豆豆
閩江學(xué)院材料與化學(xué)工程學(xué)院,福州 350108
Author(s):
LIANG Ling FANG Doudou
College of Materials and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108,China
關(guān)鍵詞:
黑磷 腫瘤微環(huán)境 腺苷激酶 活性調(diào)控
Keywords:
black phosphorus tumor microenvironment adenosine kinase activity regulation
分類號(hào):
O643; Q555
DOI:
10.20148/j.fmj.2025.02.014
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 基于磷酸鹽(PO43-)調(diào)控的腺苷激酶(ADK)活性,探究黑磷納米片(BPNSs)的選擇性降解對(duì)ADK活性的調(diào)控。方法 利用中性和酸性兩種溶液分別模擬正常生理環(huán)境、腫瘤微環(huán)境,通過吸收強(qiáng)度及PO43-含量測定探究BPNSs在兩種溶液中的降解特性; 并通過酶促反應(yīng)動(dòng)力學(xué)探究BPNSs在兩種溶液中對(duì)ADK催化腺苷磷酸化反應(yīng)的調(diào)控。結(jié)果 BPNSs在模擬的腫瘤微環(huán)境中更易降解,產(chǎn)生的PO43-明顯高于模擬的正常生理環(huán)境。ADK在兩種溶液中均能催化腺苷磷酸化,然而其活性受高濃度腺苷抑制。受益于在模擬的腫瘤微環(huán)境中降解產(chǎn)生高含量的PO43-,BPNSs恢復(fù)了ADK活性并促進(jìn)了ADK催化的腺苷轉(zhuǎn)化。結(jié)論 基于黑磷納米材料降解特性和酶活性調(diào)控機(jī)制,BPNSs有望成為新型腺苷代謝調(diào)控藥物和腺苷介導(dǎo)的免疫抑制調(diào)節(jié)劑。
Abstract:
Objective To investigate the selective degradation of black phosphorus nanosheets(BPNSs)on the regulation of adenosine kinase(ADK)activity regulated by phosphate(PO43-).Methods Neutral and acidic solutions were used to simulate normal physiological environment and tumor microenvironment, respectively.The degradation of BPNSs in both solutions were explored by measuring absorption intensity and PO43-.The regulation of BPNSs on ADK catalyzed adenosine phosphorylation in both solutions was investigated through the kinetic of enzyme-catalyzed reaction.Results In the simulated tumor microenvironment, BPNSs were degraded more easily and produced higher concentrations of PO43-.ADK could catalyze adenosine phosphorylation in both solutions, but its activity was affected by high concentration of adenosine.Benefiting from the degradation to produce high level of PO43-, BPNSs restored ADK activity and promoted ADK-catalyzed adenosine transformation in the simulated tumor microenvironment.Conclusion Based on the degradation of black phosphorus and the regulation mechanism of enzyme activity, BPNSs may become novel adenosine metabolism regulating drugs and adenosine-mediated immunosuppressive regulators.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(S202210395055); 福建省中青年教師教育科研項(xiàng)目(JAT220328); 福建省自然科學(xué)基金項(xiàng)目(2023J05245)
更新日期/Last Update: 2025-02-20