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[1]楊國(guó)華,鄭圣斌,游治杰,等.FGF19因子促進(jìn)結(jié)腸癌細(xì)胞增殖及遷移的機(jī)制研究[J].福建醫(yī)藥雜志,2024,46(05):56-59.[doi:10.20148/j.fmj.2024.05.016]
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FGF19因子促進(jìn)結(jié)腸癌細(xì)胞增殖及遷移的機(jī)制研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年05期
頁(yè)碼:
56-59
欄目:
基礎(chǔ)研究
出版日期:
2024-10-15

文章信息/Info

文章編號(hào):
1002-2600(2024)05-0056-04
作者:
楊國(guó)華鄭圣斌游治杰林娟
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院南院,福州 350007
關(guān)鍵詞:
結(jié)腸癌 FGF19 增殖 遷移
分類號(hào):
R735.3
DOI:
10.20148/j.fmj.2024.05.016
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 分析FGF19在結(jié)腸癌中的表達(dá)、對(duì)預(yù)后的影響及可能的機(jī)制。方法 通過(guò)UALCAN平臺(tái)分析FGF19在結(jié)腸癌中的表達(dá)情況; 通過(guò)Kaplan-Meier plotter平臺(tái)分析FGF19對(duì)結(jié)腸癌生存時(shí)間(OS)及無(wú)復(fù)發(fā)生存時(shí)間(RFS)的影響。細(xì)胞實(shí)驗(yàn)分3組:對(duì)照組、FGF19組、FGF19+FGF401組,CCK8法比較第5天時(shí)細(xì)胞A值和劃痕實(shí)驗(yàn)觀察24 h后遷移率,Western blot觀察3組細(xì)胞內(nèi)AKT、ERK、STAT3、pAKT、pERK、pSTAT3相對(duì)表達(dá)量。結(jié)果 FGF19在結(jié)腸癌中的表達(dá)明顯高于正常結(jié)腸組織,差異有統(tǒng)計(jì)學(xué)意義(P<0.05); FGF19降低OS及RFS,與低表達(dá)組比較,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。第5天時(shí)FGF19組細(xì)胞A值(2.76±0.18)、對(duì)照組A值(1.36±0.04)、FGF19+FGF401組A值(1.70±0.03),3組比較,差異有統(tǒng)計(jì)學(xué)意義(F=101.5,P<0.01); 而FGF19組24 h遷移率(0.56±0.04)、對(duì)照組(0.38±0.14)、FGF19+FGF401組遷移率(0.40±0.05),3組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(F=2.699,P>0.05); FGF19組pAKT(0.24±0.03)、對(duì)照組(0.20±0.02)與FGF19抑制組pAKT(0.07±0.10)比較,差異有統(tǒng)計(jì)學(xué)意義(F=5.858,P<0.05); FGF19組pSTAT3(0.08±0.006)、對(duì)照組(0.09±0.02)、FGF19+FGF401組pSTAT3(0.003±0.001),3組比較,差異有統(tǒng)計(jì)學(xué)意義(F=66.84,P<0.01)。結(jié)論 FGF19可能通過(guò)AKT、STAT3蛋白磷酸化,促進(jìn)結(jié)腸癌生長(zhǎng)及遷移,降低結(jié)腸癌患者的OS及PFS。

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省衛(wèi)健康委中青年骨干人才培養(yǎng)項(xiàng)目(2020GGB007)
更新日期/Last Update: 2024-10-15