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[1]周子杰,張家豪,姜吉霖,等.miR-586通過SFRP1調(diào)控骨肉瘤細胞增殖、遷移、侵襲的機制研究[J].福建醫(yī)藥雜志,2024,46(01):115-119.[doi:10.20148/j.fmj.2024.01.032]
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miR-586通過SFRP1調(diào)控骨肉瘤細胞增殖、遷移、侵襲的機制研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年01期
頁碼:
115-119
欄目:
基礎(chǔ)研究
出版日期:
2024-02-15

文章信息/Info

文章編號:
1002-2600(2024)01-0115-05
作者:
周子杰12張家豪2姜吉霖12李書林23付解輝4湯發(fā)強123
1 福建中醫(yī)藥大學 中西醫(yī)結(jié)合學院,福州350122;2 福建醫(yī)科大學 省立臨床醫(yī)學院,福州 350001;3 福建省立醫(yī)院骨一科,福州 350001;4 福建中醫(yī)藥大學 附屬康復醫(yī)院,福州350122
關(guān)鍵詞:
骨肉瘤微小RNA分泌型卷曲相關(guān)蛋白1SFRP1
分類號:
R738
DOI:
10.20148/j.fmj.2024.01.032
文獻標志碼:
A
摘要:
目的探究miR-586調(diào)控人骨肉瘤細胞U2OS的生物學行為的可能機制。方法通過比較骨肉瘤細胞系和人成骨細胞中miR-586的差異表達篩選目標細胞株,干擾miR-586表達后檢測其對目標細胞株增殖、遷移、侵襲能力的影響;用雙熒光素酶實驗、實時熒光定量聚合酶鏈式反應、Western blot驗證miR-586與SFRP1相關(guān)性,檢測干預miR-586、SFRP1后對目標細胞株遷移、侵襲能力的影響。結(jié)果相較于人成骨細胞hFOB1.19,骨肉瘤細胞系中U2OS細胞的miR-586表達量顯著增加;調(diào)低miR-586后U2OS細胞增殖、侵襲、遷移能力下降,SFRP1的mRNA及蛋白表達增加;過表達miR-586后U2OS細胞增殖、侵襲、遷移能力上升,SFRP1的mRNA及蛋白表達下降;同時調(diào)低miR-586、SFRP1后,U2OS細胞侵襲及遷移能力較敲低miR-586組上升。結(jié)論在本研究中,miR-586/ SFRP1軸調(diào)控骨肉瘤細胞U2OS的增殖與生物學行為,具有作為骨肉瘤的潛在診斷、治療標志物的可能性。

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備注/Memo

備注/Memo:
通信作者:湯發(fā)強,Email:[email protected]
更新日期/Last Update: 2024-02-15