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[1]林潔,廖心成,錢(qián)彬.和厚樸酚抑制IDO1表達(dá)改善老年小鼠術(shù)后認(rèn)知功能障礙的實(shí)驗(yàn)研究[J].福建醫(yī)藥雜志,2023,45(06):111-115.
 LIN Jie,LIAO Xincheng,QIAN Bin.Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice[J].FUJIAN MEDICAL JOURNAL,2023,45(06):111-115.
點(diǎn)擊復(fù)制

和厚樸酚抑制IDO1表達(dá)改善老年小鼠術(shù)后認(rèn)知功能障礙的實(shí)驗(yàn)研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年06期
頁(yè)碼:
111-115
欄目:
基礎(chǔ)研究
出版日期:
2023-12-15

文章信息/Info

Title:
Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice
文章編號(hào):
1002-2600(2023)06-0111-05
作者:
林潔廖心成1錢(qián)彬2
福建中醫(yī)藥大學(xué)附屬人民醫(yī)院麻醉科(福州 350004)
Author(s):
LIN Jie LIAO Xincheng QIAN Bin
Department of Anesthesiology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
關(guān)鍵詞:
認(rèn)知功能障礙 和厚樸酚 色氨酸代謝 吲哚胺23雙加氧酶1(IDO1)
Keywords:
cognitive dysfunction honokiol tryptophan metabolism indoleamine 23-dioxygenase 1
分類(lèi)號(hào):
R589
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討和厚樸酚對(duì)術(shù)后認(rèn)知功能障礙防治作用的機(jī)制。方法 將18月齡雄性C57BL/6小鼠隨機(jī)分為4組:Control組(溶劑預(yù)處理7 d,不進(jìn)行手術(shù))、HNK組(10 mg/kg和厚樸酚預(yù)處理7 d,不進(jìn)行手術(shù))、Surgery組(溶劑預(yù)處理7 d,脾切除術(shù)建模)和Surgery+HNK組(10 mg/kg和厚樸酚預(yù)處理7 d,脾切除術(shù)建模)。運(yùn)用Morris水迷宮評(píng)估空間記憶功能,免疫熒光染色觀察海馬凋亡神經(jīng)元比例,檢測(cè)海馬Bcl-2、Bax、活化Caspase-3以及吲哚胺2,3雙加氧酶1(indoleamine 2,3-dioxygenase 1, IDO1)表達(dá)水平,高效液相色譜檢測(cè)色氨酸毒性代謝產(chǎn)物含量。結(jié)果 與Surgery組相比,Surgery+HNK組水迷宮測(cè)試期第1、3、7天目標(biāo)象限停留時(shí)間和平臺(tái)穿越次數(shù)增加; 術(shù)后第7天海馬凋亡神經(jīng)元比例降低; 術(shù)后第1天Bcl-2表達(dá)增高、Bax表達(dá)水平降低、活化Caspase-3水平降低,IDO1表達(dá)降低; 色氨酸毒性代謝物降低。結(jié)論 和厚樸酚可通過(guò)抑制IDO1表達(dá),調(diào)控色氨酸代謝,減輕海馬神經(jīng)元損傷,改善小鼠術(shù)后認(rèn)知功能障礙。
Abstract:
Objective To explore the mechanism by which honokiol improves postoperative cognitive dysfunction(POCD)in elderly mice.Methods The 18-month-old male C57BL/6J mice were randomly divided into four groups: control group, HNK group, surgery group and surgery+HNK group.We conducted splenectomy to establish a POCD animal model.The Morris water maze(MWM)test was performed to assess the hippocampus-related cognitive function.TdT-mediated dUTP nick end labeling(TUNEL)staining was used to assess the proportion of apoptotic neurons of the hippocampus at 24 h postope-ratively.The hippocampal levels of Bcl-2, Bax, cleaved caspase-3, and indoleamine 2,3-dioxygenase 1(IDO1)were evaluated using western blotting.The contents of tryptophan metabolites kynurenine, 3-hydroxykynurenine(3-HK), 3-hydroxyanthranilic acid(3-HAA), and quinolinic acid(QA)were also detected using high performance liquid chromatography(HPLC).Results Compared with the surgery group, the surgery+HNK group mice showed increased target quadrant residence time and platform crossing times on the 1st, 3rd, and 7th day of the MWM testing period; The proportion of apoptotic neurons of hippocampal decreased in the surgery+HNK group on postoperative day 7; Honokiol pretreatment increased the level of Bcl-2 and decreased the levels of Bax, cleaved caspase-3, and IDO1 in the hippocampus on postoperative day 1.The HPLC results showed that honokiol pretreatment also reduced the contents of kynurenine, 3-HK, 3-HAA and QA in the hippocampus on postoperative day 1.Conclusion Honokiol alleviates hippocampal neuronal damage and improves POCD in elderly mice by inhibiting IDO1 expression and regulating hippocampal tryptophan metabolism.

參考文獻(xiàn)/References:

[1] Fodale V, Santamaria L, Schifilliti D, et al.Anaesthetics and postoperative cognitive dysfunction: a pathological mechanism mimicking Alzheimer's disease [J].Anaesthesia, 2010, 65(4): 388-395.
[2] Skvarc D, Berk M, Byrne L, et al.Post-operative cognitive dysfunction: an exploration of the inflammatory hypothesis and novel therapies [J].Neuroscience and Biobehavioral Reviews, 2018(84): 116-133.
[3] Garrison A M, Parrott J M, Tuñon A, et al.Kynurenine pathway metabolic balance influences microglia activity: targeting kynurenine monooxygenase to dampen neuroinflammation[J].Psychoneuroendocrinology, 2018(94):1-10.
[4] Boros F A, Klivényi P, Toldi J, et al.Indoleamine 2,3-dioxygenase as a novel therapeutic target for huntington's disease [J].Expert Opin Ther Targets, 2019, 23(1): 39-51.
[5] Göttert R, Fidzinski P, Kraus L, et al.Lithium inhibits tryptophan catabolism via the inflammation-induced kynurenine pathway in human microglia [J].Glia, 2022, 70(3): 558-571.
[6] Huang Y S, Ogbechi J, Clanchy F I, et al.IDO and kynurenine metabolites in peripheral and CNS disorders [J].Front Immunol, 2020(11): 388.
[7] Talarek S, Listos J, Barreca D, et al.Neuroprotective effects of honokiol: from chemistry to medicine[J].Biofactors, 2017, 43(6): 760-769.
[8] Zhang B, Wang P P, Hu K L, et al.Antidepressant-like effect and mechanism of action of honokiol on the mouse lipopolysaccharide(LPS)depression model[J].Molecules, 2019, 24(11):2035.
[9] Ye J S, Chen L, Lu Y Y, et al.SIRT3 activator honokiol ameliorates surgery/anesthesia-induced cognitive decline in mice through anti-oxidative stress and anti-inflammatory in hippocampus [J].CNS Neurosci Ther,2019, 25(3):355-366.
[10] Ye J S, Chen L, Lu Y Y, et al.Honokiol-mediated mitophagy ameliorates postoperative cognitive impairment induced by surgery/sevoflurane via inhibiting the activation of NLRP3 inflammasome in the hippocampus[J].Oxid Med Cell Longev, 2019, 2019:8639618.
[11] Lin D, Zuo Z.Isoflurane induces hippocampal cell injury and cognitive impairments in adult rats [J].Neuropharmacology, 2011, 61(8):1354-1359.
[12] Hovens I B, Schoemaker R G, van der Zee E A, et al.Surgery-induced behavioral changes in aged rats [J].Exp Gerontol, 2013, 48(11):1204-1211.
[13] Qu C, Li Q P, Su Z R, et al.Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer's disease via inhibiting neuropathology and modulating gut microbiota[J].J Adv Res, 2021(35):231-243.
[14] Guo S, Xu J J, Wei N, et al.Honokiol attenuates the memory impairments, oxidative stress, neuroinflammation, and GSK-3β activation in vascular dementia rats [J].J Alzheimers Dis, 2019, 71(1):97-108.
[15] Lu S M, Gui B, Dong H Q, et al.Prophylactic lithium alleviates splenectomy-induced cognitive dysfunction possibly by inhibiting hippocampal TLR4 activation in aged rats [J].Brain Res Bull, 2015(114):31-41.
[16] Bettio L E B, Rajendran L, Gil-Mohapel J.The effects of aging in the hippocampus and cognitive decline [J].Neurosci Biobehav Rev, 2017(79):66-86.
[17] Kotekar N, Shenkar A, Nagaraj R.Postoperative cognitive dysfunction-current preventive strategies [J].Clin Interv Aging, 2018, 8(13):2267-2273.
[18] Gao R, Kan M Q, Wang S G, et al.Disrupted tryptophan metabolism induced cognitive impairment in a mouse model of sepsis-associated encephalopathy [J].Inflammation, 2016, 39(2):550-560.
[19] Mor A, Tankiewicz-Kwedlo A, Krupa A, et al.Role of kynurenine pathway in oxidative stress during neurodegenerative disorders [J].Cells, 2021, 10(7):1603.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省中青年教師教育科研項(xiàng)目(JAT220114); 福建省自然科學(xué)基金(2022J01847)
1 福建中醫(yī)藥大學(xué)附屬第二人民醫(yī)院麻醉科; 2 通信作者
更新日期/Last Update: 2023-12-15