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[1]張良志,劉,洪,等.基于NLRP3/caspase-1通路探討針刀對兔膝骨關(guān)節(jié)炎軟骨細(xì)胞的影響[J].福建醫(yī)藥雜志,2023,45(05):87-90.
　ZHANG Liangzhi,LIU Hong,XIE Ziyi,et al.Effect of acupotomy on chondrocytes in rabbit knee osteoarthritis based on NLRP3/caspase-1 pathway[J].FUJIAN MEDICAL JOURNAL,2023,45(05):87-90.
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基于NLRP3/caspase-1通路探討針刀對兔膝骨關(guān)節(jié)炎軟骨細(xì)胞的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年05期
頁碼:
87-90
欄目:
基礎(chǔ)研究
出版日期:
2023-10-15

文章信息/Info

Title:
Effect of acupotomy on chondrocytes in rabbit knee osteoarthritis based on NLRP3/caspase-1 pathway
文章編號:
1002-2600(2023)05-0087-04
作者:
張良志1; 2; 劉; 洪1; 2; 謝梓毅3; 李陽陽3; 余文英3; 劉晶1; 修忠標(biāo)1; 2; 3; 4
福建中醫(yī)藥大學(xué)附屬人民醫(yī)院骨傷二科(福州 350004)
Author(s):
ZHANG Liangzhi;  LIU Hong;  XIE Ziyi;  LI Yangyang;  YU Wenying;  LIU Jin;  XIU Zongbiao
Second Department of Orthopedics-traumatology, The People's Hospital Affiliated of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
關(guān)鍵詞:
膝骨關(guān)節(jié)炎;  針刀;  NLRP3/Caspase-1通路;  軟骨細(xì)胞焦亡
Keywords:
kneeosteoarthritis(KOA);  acupotomy;  NLRP3/Caspase-1 pathway;  chondrocyte pyroptosis
分類號:
R681.3
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 觀察針刀對膝骨關(guān)節(jié)炎(KOA)兔軟骨NLRP3/Caspase-1通路的調(diào)控作用及軟骨細(xì)胞焦亡的影響。方法 選用24只新西蘭兔雄兔,年齡均為6個月。使用隨機(jī)數(shù)字表法將其分為3組,分別為空白組、模型組、針刀組,每組各8只。造模方法采用改良Videman法進(jìn)行固定,造模時間為6周,空白組不予固定。針刀組選取經(jīng)筋病灶點(diǎn)行針刀松解治療,每周1 次,共治療3 次; 空白組和模型組正常飼養(yǎng),行同樣抓捕行為。采用HE染色觀察軟骨形態(tài)學(xué)改變; 并檢測軟骨Caspase-1、NLRP3、ASC、IL-1βmRNA和蛋白的表達(dá)。結(jié)果 空白組軟骨細(xì)胞均勻地分布在軟骨基質(zhì)中,表面相對光滑,層次結(jié)構(gòu)較為清晰,潮線清晰完整。模型組軟骨細(xì)胞數(shù)量較少,且分布狀態(tài)較不均勻,表層出現(xiàn)缺損,潮線紊亂。與模型組相比,針刀組軟骨表面較光滑,且軟骨細(xì)胞分布較均勻,排列較規(guī)整,潮線較完整。與空白組比較,模型組軟骨組織Caspase-1、NLRP3、IL-1β mRNA表達(dá)顯著上調(diào)(P<0.05); 與模型組比較,針刀組軟骨組織Caspase-1、NLRP3、IL-1β mRNA表達(dá)顯著下調(diào)(P<0.05)。與空白組比較,模型組中兔軟骨組織Caspase-1、NLRP3、IL-1β mRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表達(dá)水平顯著升高(P<0.05)。針刀干預(yù)后兔軟骨細(xì)胞排列較模型組改善,軟骨細(xì)胞數(shù)量增多,Caspase-1、NLRP3、IL-1β mRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表達(dá)水平顯著下調(diào)(P<0.05)。結(jié)論 基于經(jīng)筋理論運(yùn)用針刀治療KOA,延緩軟骨病變,從而改善癥狀,其作用機(jī)制可能是通過干預(yù)軟骨細(xì)胞焦亡,從而降低關(guān)節(jié)內(nèi)炎癥介質(zhì)來實(shí)現(xiàn)的。
Abstract:
Objective To observe the effects of acupotomy on the regulation of the NLRP3/Caspase-1 pathway and chondrocyte pyroptosis in rabbit cartilage with knee osteoarthritis(KOA).Methods Twenty-four male New Zealand rabbits, all aged six months, were used.They were divided into three groups using the random number table method: blank group, model group, and acupotomy group.The modeling method was fixed by the modified Videman method for six weeks, and the blank group was not fixed.In the acupotomy group, the transversal tendon lesion was treated with needle knife, once a week, for a total of three times; the blank group and the model group were not treated.The morphological changes of cartilage were observed by HE staining, and the expression of cartilage Caspase-1, NLRP3, ASC and IL-1β in the cells were detected.Results The chondrocytes in the blank group were uniformly distributed in the cartilage matrix, with relatively smooth surface, clearer hierarchical structure and clear and complete tide lines.In the model group, the number of chondrocytes was smaller, and the distribution was uneven, with defects in the surface layer and disturbed tide lines.Compared with the model group, the cartilage surface of the needle knife group was smoother, and the chondrocytes were more evenly distributed and arranged, and the tide lines were more complete.Compared with the blank group, cartilage tissue Caspase-1, NLRP3, IL-1β mRNA expression was significantly up-regulated in the model group(P<0.05); compared with the model group, cartilage tissue Caspase-1, NLRP3, IL-1β mRNA expression was significantly down-regulated in the needle-knife group(P<0.05).Compared with the blank group, the expression levels of Caspase-1, NLRP3, IL-1β mRNA and Caspase-1, NLRP3, IL-1β, ASC protein in rabbit cartilage tissue were significantly increased in the model group(P<0.05).Chondrocyte alignment was improved in rabbits after acupotomy intervention compared with the model group, the number of chondrocytes was increased, and the expression levels of Caspase-1, NLRP3, IL-1β mRNA and Caspase-1, NLRP3, IL-1β, ASC protein were significantly downregulated(P<0.05).Conclusion Treating KOA with acupotomy based on the theory of meridian theory can delay cartilage lesions and improve symptoms.The mechanism of action may be reducing the intra-articular inflammatory mediators by intervening chondrocyte pyroptosis.

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相似文獻(xiàn)/References:

[1]張良志,劉洪,楊金碩,等.針刀治療膝骨性關(guān)節(jié)炎的臨床療效及機(jī)制研究[J].福建醫(yī)藥雜志,2024,46(02):18.[doi:10.20148/j.fmj.2024.02.005]
　ZHANG Liangzhi,LIU Hong,YANG Jinshuo,et al.Clinical efficacy and mechanism of acupotomy in the treatment of knee osteoarthritis[J].FUJIAN MEDICAL JOURNAL,2024,46(05):18.[doi:10.20148/j.fmj.2024.02.005]
[2]鄭世雄,林煜,劉合亮,等.基于PI3K/Akt通路探討氟尿嘧啶對大鼠軟骨細(xì)胞凋亡的影響[J].福建醫(yī)藥雜志,2024,46(03):99.[doi:10.20148/j.fmj.2024.03.031]
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備注/Memo

備注/Memo:
基金項目:福建省自然科學(xué)基金面上項目(2022J01354); 福建中醫(yī)藥大學(xué)中醫(yī)骨傷及運(yùn)動康復(fù)教育部重點(diǎn)實(shí)驗(yàn)室開放課題(ZD2020-1-4)
1 福建省骨傷研究所; 2 中醫(yī)骨傷及運(yùn)動康復(fù)教育部重點(diǎn)實(shí)驗(yàn)室; 3 福建中醫(yī)藥大學(xué); 4 通信作者,Email:[email protected]
更新日期/Last Update: 2023-10-15