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[1]鄭俊杰,陳冬冬,林佳生,等.褪黑素對骨質疏松大鼠骨代謝及成骨細胞Wnt/β-catenin信號通路的影響[J].福建醫(yī)藥雜志,2023,45(04):111-114.
　ZHENG Junjie,CHEN Dongdong,LIN Jiasheng,et al.Effect of melatonin on bone metabolism and osteoblast Wnt/β-catenin signaling pathway in osteoporotic rats[J].FUJIAN MEDICAL JOURNAL,2023,45(04):111-114.
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褪黑素對骨質疏松大鼠骨代謝及成骨細胞Wnt/β-catenin信號通路的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年04期
頁碼:
111-114
欄目:
基礎研究
出版日期:
2023-08-15

文章信息/Info

Title:
Effect of melatonin on bone metabolism and osteoblast Wnt/β-catenin signaling pathway in osteoporotic rats
文章編號:
1002-2600(2023)04-0111-04
作者:
鄭俊杰; 陳冬冬; 林佳生; 嚴偉; 洪嘉祥; 宋平; 陳嶸1
福建省福州市第二醫(yī)院骨與軟組織腫瘤科(福州 350007)
Author(s):
ZHENG Junjie;  CHEN Dongdong;  LIN Jiasheng;  YAN Wei;  HONG Jiaxiang;  SONG Ping;  CHEN Rong
Department of Bone and Soft Tissue Oncology, Fuzhou Second Hospital, Fuzhou, Fujian 350007, China
關鍵詞:
褪黑素;  骨質疏松;  骨代謝;  Wnt/β-catenin
Keywords:
melatonin;  osteoporosis;  bone metabolism;  Wnt/β-catenin
分類號:
R58
文獻標志碼:
B
摘要:
目的 探討褪黑素對骨質疏松大鼠骨代謝及成骨細胞Wnt/β-catenin信號通路的影響。方法 按照隨機數(shù)字表法將60只大鼠分成空白組、模型組和褪黑素組。空白組使用與褪黑素同等劑量的生理鹽水進行灌胃; 模型組在空白組的基礎上行雙側卵巢切除術,構建骨質疏松大鼠模型; 褪黑素組在模型組的基礎上,采用靜脈注射的方式給予褪黑素治療。采用ELISA法檢測3組大鼠血清中堿性磷酸酶(ALP)、Ⅰ型前膠原氨基端原肽(PINP)、骨鈣素(OC)、I型膠原C端交聯(lián)肽(CTX-Ⅰ)的含量。采用RT-PCR及Western blot法檢測大鼠成骨細胞中Wnt/β-catenin信號通路相關因子細胞β-連環(huán)蛋白(β-catenin)、無翅型MMTV整合位點家族成員3A(Wnt3a)、無翅型MMTV整合位點家族成員7B(Wnt7b)、軸抑制蛋白2抗體(Axin2)的mRNA及蛋白表達。結果 與模型組比較,褪黑素組大鼠血清中的ALP、PINP、OC、CTX-Ⅰ含量得到提升,差異有統(tǒng)計學意義(P<0.05)。與模型組比較,褪黑素組大鼠成骨細胞的β-catenin、Wnt7b的mRNA和蛋白含量明顯升高(P<0.05),Axin2的mRNA和蛋白含量明顯降低(P<0.05)。結論 褪黑素能夠調節(jié)骨代謝平衡,并通過激活Wnt/β-catenin信號通路促進成骨細胞的形成。
Abstract:
Objective To investigate the effect of melatonin on bone metabolism and osteoblast Wnt /β-catenin signaling pathway in osteoporotic rats. Methods According to the random number table method,60 rats were divided into blank group,model group and melatonin group. The blank group was intragastrically administered with the same dose of normal saline as melatonin; the model group underwent bilateral ovariectomy on the basis of the blank group to construct a rat model of osteoporosis; on the basis of the model group, the melatonin group was treated with melatonin by intravenous injection. The contents of alkaline phosphatase(ALP), type I procollagen amino terminal propeptide(PINP), osteocalcin(OC)and type I collagen C-terminal cross-linked peptide(CTX-I)in serum of rats in the three groups were detected by ELISA. The mRNA and protein expression of Wnt/β-catenin signaling pathway related factors β-catenin,Wnt3a,Wnt7b and Axin2 in rat osteoblasts were detected by RT-PCR and Western blot. Results Compared with the model group,the contents of ALP,PINP,OC and CTX-I in the serum of the melatonin group were increased significantly(P<0.05). Compared with the model group,the mRNA and protein levels of β-catenin and Wnt7b in osteoblasts of rats in the melatonin group were significantly increased(P<0.05),and the mRNA and protein levels of Axin2 were significantly decreased(P<0.05). Conclusion Melatonin can regulate the balance of bone metabolism and promote the formation of osteoblasts by activating the Wnt/β-catenin signaling pathway.

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備注/Memo

備注/Memo:
基金項目:福建省科技創(chuàng)新平臺項目“2020年福建省創(chuàng)傷骨科急救與康復臨床醫(yī)學研究中心”(2020Y2014)
1 通信作者,Email:[email protected]
更新日期/Last Update: 2023-08-15