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[1]黃婷,程艷芳,潘銘東,等.益景湯經(jīng)PI3K/AKT/eNOS信號(hào)通路拮抗高糖誘導(dǎo)的視網(wǎng)膜血管內(nèi)皮細(xì)胞損傷[J].福建醫(yī)藥雜志,2023,45(02):99-103.
　HUANG Ting,CHENG Yanfang,PAN Mingdong,et al.Yijing Decoction inhibits retinal microvascular endothelial cell injury induced by high glucose through PI3K/AKT/eNOS signal pathway[J].FUJIAN MEDICAL JOURNAL,2023,45(02):99-103.
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益景湯經(jīng)PI3K/AKT/eNOS信號(hào)通路拮抗高糖誘導(dǎo)的視網(wǎng)膜血管內(nèi)皮細(xì)胞損傷()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年02期
頁(yè)碼:
99-103
欄目:
基礎(chǔ)研究
出版日期:
2023-04-15

文章信息/Info

Title:
Yijing Decoction inhibits retinal microvascular endothelial cell injury induced by high glucose through PI3K/AKT/eNOS signal pathway
文章編號(hào):
1002-2600(2023)02-0099-05
作者:
黃婷; 程艷芳; 潘銘東1; 劉光輝1; 何建忠1; 2; 鄭永征1; 3
福建中醫(yī)藥大學(xué)第一臨床醫(yī)學(xué)院（福州 350004）
Author(s):
HUANG Ting;  CHENG Yanfang;  PAN Mingdong;  LIU Guanghui;  HE Jianzhong;  ZHENG Yongzheng
First Clinical Medicine College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
關(guān)鍵詞:
益景湯; 高糖; 內(nèi)皮細(xì)胞損傷; PI3K/AKT/eNOS信號(hào)通路
Keywords:
Yijing Decoction;  high glucose;  endothelial cell injury;  PI3K/AKT/eNOS signal pathway
分類號(hào):
R587.1;R744.1
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討益景湯對(duì)高糖誘導(dǎo)大鼠視網(wǎng)膜微血管內(nèi)皮細(xì)胞損傷的拮抗作用及機(jī)制。方法 將大鼠視網(wǎng)膜微血管內(nèi)皮細(xì)胞隨機(jī)分為5組：對(duì)照組（CG）、高糖組(HG)、低濃度益景湯組(LYG)、中濃度益景湯組(MYG)、高濃度益景湯組(HYG)。細(xì)胞生長(zhǎng)至80%～90%時(shí)，往高糖組及益景湯組培養(yǎng)基中加入33 mmol/L葡萄糖培養(yǎng)3 d造高糖內(nèi)皮細(xì)胞損傷模型，第4天按分組加入相應(yīng)正常糖培養(yǎng)基及各濃度益景湯培養(yǎng)24 h。使用CCK-8試劑盒檢測(cè)內(nèi)皮細(xì)胞活性，Transwell法觀察內(nèi)皮細(xì)胞遷移，熒光聚合酶鏈?zhǔn)椒磻?yīng)(PCR)檢測(cè)細(xì)胞間緊密連接蛋白ZO-1及內(nèi)皮細(xì)胞磷脂酰肌醇三羥基激酶(PI3K)、蘇氨酸蛋白激酶(AKT)、內(nèi)皮型一氧化氮合酶(eNOS)的信使RNA（mRNA）表達(dá)。結(jié)果 高糖條件下，大鼠視網(wǎng)膜微血管內(nèi)皮細(xì)胞活性降低，與對(duì)照組比較，差異有統(tǒng)計(jì)學(xué)意義（均P<0.05）；與高糖組比較，低、中、高濃度益景湯組細(xì)胞活性均高于高糖組，差異均有統(tǒng)計(jì)學(xué)意義（均P<0.05）。高糖條件下，大鼠視網(wǎng)膜微血管內(nèi)皮細(xì)胞遷移增強(qiáng)，與對(duì)照組比較，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）；與高糖組比較，低、中、高濃度益景湯組細(xì)胞遷移均低于高糖組，差異均有統(tǒng)計(jì)學(xué)意義（均P<0.05）。高糖條件下，大鼠視網(wǎng)膜微血管內(nèi)皮細(xì)胞間連接蛋白ZO-1及內(nèi)皮細(xì)胞PI3K、AKT、eNOS的mRNA表達(dá)量均降低，與對(duì)照組比較，差異均有統(tǒng)計(jì)學(xué)意義（均P<0.05）；與高糖組比較，低、中、高濃度益景湯組細(xì)胞間連接蛋白ZO-1及內(nèi)皮細(xì)胞PI3K、AKT、eNOS的mRNA表達(dá)量均高于高糖組，差異均有統(tǒng)計(jì)學(xué)意義（均P<0.05）。結(jié)論 益景湯能經(jīng)PI3K/AKT/eNOS信號(hào)通路抑制高糖誘導(dǎo)視網(wǎng)膜微血管內(nèi)皮細(xì)胞遷移，提高細(xì)胞活性，減輕細(xì)胞損傷。
Abstract:
Objective To investigate the protective effect and mechanism of Yijing Decoction on retinal vascular endothelial cell injury induced by high glucose in rats. Methods Rat retinal microvascular endothelial cells were randomly divided into five groups: control group (CG), high glucose group (HG), low concentration Yijing Decoction group (LYG), medium concentration Yijing Decoction group (MYG) and high concentration Yijing Decoction group (HYG). When the cells grew to 80%-90%, the high glucose endothelial cell injury model was established by adding 33 mmol/L glucose to the culture medium of high glucose group and Yijing Decoction groups for three days, and the corresponding normal glucose medium and Yijing Decoction of various concentrations were added for 24 hours on the 4th day. Endothelial cell viability was detected by CCK-8 kit, endothelial cell migration was observed by Transwell method, and the mRNA expression of intercellular connexin ZO-1 and endothelial cell PI3K, AKT and eNOS was detected by fluorescent PCR. Results Under the condition of high glucose, the activity of retinal microvascular endothelial cells in the control group was significantly lower than that in the control group (all P<0.05). The cell activity in the low, middle and high concentration Yijing Decoction groups were significantly higher than that in the high glucose group (all P<0.05). The migration of retinal microvascular endothelial cells in the high glucose group was significantly higher than that in the control group (P<0.05), and the cell migration in the low, middle and high concentration Yijing Decoction groups were significantly lower than that in the high glucose group (all P<0.05). Under the condition of high glucose, the mRNA expression of ZO-1, PI3K, AKT and eNOS in retinal microvascular endothelial cells were significantly lower than those in the control group. Compared with the high glucose group, the mRNA expression of intercellular connexin ZO-1 and endothelial cell PI3K, AKT and eNOS in the low, middle and high concentration Yijing Decoction groups were significantly higher than those in the high glucose group (all P<0.05). Conclusion Yijing Decoction can antagonize the expression of PI3K, AKT, eNOS and ZO-1mRNA in retinal microvascular endothelial cells of rats injured by high glucose, reduce endothelial cell migration, increase endothelial cell activity and reduce microvascular endothelial cell injury.

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備注/Memo

備注/Memo:
基金項(xiàng)目：福建省自然科學(xué)基金資助項(xiàng)目（2020J011037；2022J01352）
1 福建中醫(yī)藥大學(xué)附屬人民醫(yī)院 福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合眼科研究所；2 共同通信作者，Email:[email protected]；3 通信作者，Email：[email protected]
更新日期/Last Update: 2023-04-15