80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]林然,吳新武,黃健,等.可溶性Jagged1/Fc對大鼠骨骺干細(xì)胞Notch信號通路的影響研究[J].福建醫(yī)藥雜志,2022,44(05):130-133.
　LIN Ran,WU Xinwu HUANG Jian,CHEN Shunyou.Effect of soluble Jagged1/Fc on Notch signaling pathway in rat epiphyseal stem cells[J].FUJIAN MEDICAL JOURNAL,2022,44(05):130-133.
點(diǎn)擊復(fù)制

可溶性Jagged1/Fc對大鼠骨骺干細(xì)胞Notch信號通路的影響研究()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年05期
頁碼:
130-133
欄目:
基礎(chǔ)研究
出版日期:
2022-10-15

文章信息/Info

Title:
Effect of soluble Jagged1/Fc on Notch signaling pathway in rat epiphyseal stem cells
作者:
林然; 吳新武; 黃健; 陳順有1
福建省福州市第二醫(yī)院小兒骨科（福州 350007）
Author(s):
LIN Ran; WU Xinwu HUANG Jian; CHEN Shunyou
Department of pediatric orthopaedics, Fuzhou second hospital, Fuzhou, Fujian 350007,China
關(guān)鍵詞:
大鼠; 骨骺干細(xì)胞; Notch信號通路; Jagged1/Fc
Keywords:
rats; epiphyseal stem cells; Notch signal pathway; Jagged1/Fc
分類號:
R68
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 探究可溶性Jagged1/Fc對鼠骨骺干細(xì)胞Notch信號通路的激活及生物學(xué)特性的影響。 方法 將2周齡內(nèi)SP大鼠離體培養(yǎng)獲取大鼠骨骺干細(xì)胞，設(shè)置不同濃度（0.5 μg/mL、1 μg/mL、2 μg/mL）的Jagged1/Fc對第3代骨骺干細(xì)胞進(jìn)行干預(yù)誘導(dǎo)，體外培養(yǎng)，在倒置顯微鏡下觀察細(xì)胞生長情況,記錄細(xì)胞貼壁情況及時間、細(xì)胞形態(tài)變化及增殖情況，采用CCK-8法檢測細(xì)胞增殖活性，及使用Western印跡檢測Jagged1/Fc對骨骺干細(xì)胞增殖分化影響。 結(jié)果 經(jīng)可溶性Jagged1/Fc干預(yù)誘導(dǎo)后，與對照組比較，實驗組與對照組細(xì)胞形態(tài)變化基本類似，但濃度為0.5 μg/mL的Jagged1/Fc實驗組細(xì)胞增殖速度顯著加快,貼壁所需的時間較對照組縮短（P<0.05）.CollagenⅡ蛋白表達(dá)明顯增多,膠原蛋白X表達(dá)明顯減少。 結(jié)論 單個Jagged1/Fc配體可激活Notch信號通路，促進(jìn)骨骺干細(xì)胞增殖的最適濃度是0.5 μg/mL。
Abstract:
Objective To investigate the effect of soluble Jagged1/Fc on the activation and biological characteristics of Notch signaling pathway in rat epiphyseal stem cells. Methods Two weeks old of the SP rats were cultured in vitro to obtain rat epiphyseal stem cells. Jagged1/Fc with different concentrations (0.5 μg/mL, 1 μg/mL and 2 μg/mL) was set to intervene and induce the third generation epiphyseal stem cells. They were cultured in vitro. The cell growth,cell adhesion and time, cell morphological changes and proliferation were observed under inverted microscope, the cell proliferation activity was detected by CCK-8 method, and the effect of Jagged /Fc on the proliferation and differentiation of epiphyseal stem cells was detected by Western blot. Results After the intervention and induction of soluble Jagged1/Fc, the morphological changes of cells in the experimental group were basically similar to those in the control group, but the proliferation rate of cells in the experimental group with jagged1/Fc at the concentration of 0.5μg/mL was significantly faster and the time required for adhesion was shorter than that in the control group (P<0.05) The expression of collagen Ⅱ protein increased significantly and the expression of collagen X decreased significantly. Conclusion Single Jagged1/Fc ligand can activate Notch signaling pathway, and the optimal concentration to promote the proliferation of epiphyseal stem cells is 0.5μg/mL.

參考文獻(xiàn)/References:

[1] Ianotti J P.Growth plate physiology and pathology[J].Orthop Clin N Am，1990，21（1）:1-17.
[2] Joyce M E, Jingushi S, Scully S P,et al.Role of growth factors in fracture healing[J].Prog Clin Biol Res,1991(365):391-416.
[3] 張衣北、陳安民、郭風(fēng)勁，等.Notch1信號系統(tǒng)對骨骺干細(xì)胞增殖與分化調(diào)控作用的初步觀察[J].中華醫(yī)學(xué)雜志，2005,85(48)：3430-3434.
[4] Zanotti S, Canalis E.Notch signaling in skeletal health and disease[J].Eur J Endocrinol,2013,168(6):95-103.
[5] Regan J, Long F.Notch signaling and bone remodeling[J].Curr Osteoporos Rep,2013,11(2):126-129.
[6] Bai S, Kopan R, Zou W, et al.Notch1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells[J].J Biol Chem,2008,283(10):6509-6518.
[7] Dong Y, Jesse A M, Kohn A, et al.RBPjkappa-dependent Notch signaling regulates mesenchymal progenitor cell proliferation and differentiation during skeletal development[J].Development,2010,137(9):1461-1471.
[8] Hilton M J, Tu X, Wu X, et al.Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation[J].Nat Med,2008,14(3):306-314.
[9] Weber J M，F(xiàn)orsythe S R，Christianson C A，et a1.Parathyroid hormone stimulates expression of the Notch ligand Jaggedl in osteoblastic ceils[J].Bone，2006，39(3) ：485-493.
[10] Maillard I,Fang T，Pear W S.Regulation of lymphoid development, differentiation, and function by the Notch pathway[J].Annu Rev Immunol, 2005，23（1）：945-974.
[11] Lawal R A, Zhou X, Batey K, et al.The Notch ligand Jagged1 regulates the osteoblastic lineage by maintaining the osteoprogenitor pool[J].J Bone Miner Res,2017，32（6）：1320-3131.

相似文獻(xiàn)/References:

[1]吳世強(qiáng)[],徐杰[],林文平[].β-干擾素對大鼠急性脊髓損傷后神經(jīng)功能恢復(fù)的影響[J].福建醫(yī)藥雜志,2017,39(3):74.
　WU Shi-qiang[],XU Jie[],LIN Wenping[].Influence of β-interferon on recovery of neurological function after acute spinal cord injury in rats[J].FUJIAN MEDICAL JOURNAL,2017,39(05):74.
[2]賴躍平,劉水源,陳建松,等.TGF-β1/Smad信號通路相關(guān)因子在顱腦外傷大鼠體內(nèi)的表達(dá)研究[J].福建醫(yī)藥雜志,2017,39(4):67.

備注/Memo

備注/Memo:
基金項目：福州市科技計劃項目 “骨骺干細(xì)胞Notch信號激活下復(fù)合藻酸鹽水凝支架修復(fù)生長板損傷的實驗研究” （2019-SZ-19）;福建省科技創(chuàng)新平臺項目 “福建省創(chuàng)傷骨科急救與康復(fù)臨床醫(yī)學(xué)研究中心”（2020Y2014）
1通信作者
更新日期/Last Update: 2022-10-15