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[1]郭徽靈,胡世平,湯發(fā)強(qiáng),等.血管生成素-2在大鼠骨肉瘤細(xì)胞中的表達(dá)及調(diào)控的研究[J].福建醫(yī)藥雜志,2021,43(02):135-138.
　GUO Huiling,HU Shiping,TANG Faqiang,et al.Study on the expression and regulation of angiopoietin-2 in rat osteosarcoma cells[J].FUJIAN MEDICAL JOURNAL,2021,43(02):135-138.
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血管生成素-2在大鼠骨肉瘤細(xì)胞中的表達(dá)及調(diào)控的研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期數(shù):
2021年02期
頁碼:
135-138
欄目:
基礎(chǔ)研究
出版日期:
2021-04-15

文章信息/Info

Title:
Study on the expression and regulation of angiopoietin-2 in rat osteosarcoma cells
文章編號:
1002-2600(2021)02-0135-04
作者:
郭徽靈; 胡世平; 湯發(fā)強(qiáng); 吳 宏; 鄭建章
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院骨一科(福州 350001)
Author(s):
GUO Huiling;  HU Shiping;  TANG Faqiang;  WU Hong;  ZHENG Jianzhang
The first Department of Orthopaedics,Fujian Provincial Hospital,Provincial Clinical Medical College of Fujian Medical University,Fuzhou,Fujian 350001,China
關(guān)鍵詞:
血管生成素-2;  骨肉瘤;  大鼠模型;  細(xì)胞調(diào)控
Keywords:
angiopoietin-2;  osteosarcoma;  rat model;  cell regulation
分類號:
R738.1
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 觀察血管生成素-2(Ang-2)在大鼠骨肉瘤建模中不同時(shí)期的表達(dá)及其對骨肉瘤細(xì)胞的調(diào)控作用。方法 20只大鼠股骨下段注射MG63骨肉瘤細(xì)胞,成瘤后分為5、10、15、20 d組,5只股骨下段注射等量生理鹽水的大鼠為對照組。測定各組大鼠血清Ang-2含量,并對成瘤行免疫組化染色證實(shí)。構(gòu)建敲減Ang-2基因表達(dá)的shRNA慢病毒載體,轉(zhuǎn)染至培養(yǎng)的MG63骨肉瘤細(xì)胞中,觀察對骨肉瘤細(xì)胞周期及凋亡的影響。結(jié)果 實(shí)驗(yàn)組血清Ang-2在4個(gè)不同階段均較對照組明顯升高,其中10 d組上升幅度最大(P值均<0.05); 免疫組化染色發(fā)現(xiàn),成瘤10 d組Ang-2表達(dá)陽性細(xì)胞數(shù)最高,且與血清測定結(jié)果相符; 抑制Ang-2表達(dá)使骨肉瘤細(xì)胞分裂阻滯在G1期,并增加細(xì)胞凋亡(P<0.05)。結(jié)論 Ang-2與骨肉瘤發(fā)生發(fā)展關(guān)系密切,可能成為骨肉瘤早期診斷及潛在治療的一個(gè)新的參考指標(biāo)。
Abstract:
Objective To observe the expression of angiogenin-2(Ang-2)at different stages in rat osteosarcoma modeling and its regulation effect on osteosarcoma cells. Methods MG63 osteosarcoma cells were injected into the lower femoral segment of 20 rats,and after tumor formation, the rats were divided into 5, 10, 15, and 20 days groups. Five rats were injected with the same amount of normal saline in the lower femoral segment as the control group.The serum Ang-2 content in each group was determined and confirmed by immunohistochemical staining.ShRNA lentivirus vector with ang-2 gene knockdown was constructed and transfected into cultured MG63 osteosarcoma cells to observe the effects on cell cycle and apoptosis of osteosarcoma cells. Results The serum Ang-2 level in the experimental group was significantly higher than that in the control group at four different stages, with the maximum increase in the 10-days group(all P<0.05).Immunohistochemical staining showed that the number of positive ang-2 cells was the highest in the 10-days tumorigenicity group, which was consistent with the serum test results.Inhibition of Ang-2 expression blocked cell division in the G1 phase and increased apoptosis(P<0.05). Conclusion Ang-2 is closely related to the occurrence and development of osteosarcoma,and may become a new reference index for early diagnosis and potential treatment of osteosarcoma.

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備注/Memo

備注/Memo:
基金項(xiàng)目:2018年省衛(wèi)生計(jì)生青年科研課題資助項(xiàng)目(2018-1-6)
更新日期/Last Update: 2021-04-15