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[1]陳炳基 蔣小燕 楊建勝.HO-1過表達(dá)抑制NLRP1通路活化在脊髓損傷中發(fā)揮保護(hù)作用的實(shí)驗(yàn)研究[J].福建醫(yī)藥雜志,2020,42(02):115-119.
　CHEN Bingji,JIANG Xiaoyan,YANG Jiansheng..Experimental study on the protective effect of HD-1 overexpression on NLRP1 pathway activation in spinal cord injury[J].FUJIAN MEDICAL JOURNAL,2020,42(02):115-119.
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HO-1過表達(dá)抑制NLRP1通路活化在脊髓損傷中發(fā)揮保護(hù)作用的實(shí)驗(yàn)研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年02期
頁碼:
115-119
欄目:
基礎(chǔ)研究
出版日期:
2020-04-15

文章信息/Info

Title:
Experimental study on the protective effect of HD-1 overexpression on NLRP1 pathway activation in spinal cord injury
文章編號(hào):
1002-2600(2020)02-0115-05
作者:
陳炳基 蔣小燕 楊建勝1
福建醫(yī)科大學(xué)附屬第二醫(yī)院急診科(泉州 362000)
Author(s):
CHEN Bingji; JIANG Xiaoyan;  YANG Jiansheng.
Emergency Department, the Second Affiliated Hospital of Fujian Medical University, Quanzhou,Fujian362000,China
關(guān)鍵詞:
脊髓損傷;  血紅素加氧酶-1;  炎性小體;  基因治療
Keywords:
spinal cord injury;  heme oxygenase-1;  inflammasome;  gene therapy
分類號(hào):
R68
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討血紅素加氧酶-1(HO-1)在脊髓損傷(SCI)中的保護(hù)作用及其機(jī)制。方法 采用壓迫損傷法構(gòu)建SCI模型,將大鼠隨機(jī)分為假手術(shù)組(Sham組)、單純SCI組(Vehicle組)、腺相關(guān)病毒感染SCI大鼠組(A-C組)和過表達(dá)HO-1的腺相關(guān)病毒感染SCI大鼠組(A-HO-l組)。Western blot檢測(cè)HO-1、NLRP1、NLRP3、NLRC4和caspase-1的表達(dá),免疫熒光檢測(cè)HO-1和MAP2的表達(dá)定位,原位缺口末端標(biāo)記法(TUNEL)檢測(cè)脊髓組織細(xì)胞凋亡,后肢行為學(xué)(Basso-Beattie-Bresnahan,BBB)評(píng)分評(píng)估大鼠后肢運(yùn)動(dòng)功能。結(jié)果 SCI后HO-1表達(dá)增加,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。與A-C組相比,A-HO-1組中HO-1水平明顯升高,NLRP1、pro-caspase-1、caspase-1中p20表達(dá)水平和凋亡細(xì)胞數(shù)顯著降低,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。損傷后3、7、14、21 d,A-HO-1組大鼠BBB評(píng)分較Vehicle組和A-C組顯著升高,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 過表達(dá)HO-1通過抑制NLRP1炎性復(fù)合物激活而減少SCI后細(xì)胞凋亡和改善大鼠后肢運(yùn)動(dòng)功能,對(duì)SCI大鼠起保護(hù)作用。
Abstract:
Objective To investigate the protective effect of heme oxygenase-1(HO-1)on spinal cord injury(SCI)and its mechanism.Methods SCI model was established by compression injury, and the rats were randomly divided into sham group, simple SCI group(Vehicle group), adeno-associated virus infected SCI group(A-C group)and adeno-associated virus overexpressing HO-1 infected SCI group(A-HO-l group).The expression of HO-1, NLRP1, NLRP3, NLRC4 and caspase-1 was detected by Western blot, HO-1 and MAP2 expression were detected by immunofluorescence, cell apoptosis of spinal cord tissues was measured by TUNEL, and motor function of hind limbs was evaluated by Basso-Beattie-Bresnahan(BBB)score.Results The expression of HO-1 was increased significantly after SCI(P<0.05).Compared with A-C group, the expression of HO-1 increased significantly, the expression of NLRP1、pro-caspase-1、caspase-1 p20 and the proportion of apoptotic cells decreased significantly in A-HO-1(P<0.05)group.The BBB score was significantly higher in the A-HO-1 group than that in the Vehicle and A-C groups at 3, 7, 14, and 21 days after SCI(P<0.05).Conclusion Overexpression of HO-1 can protect SCI rats by inhibiting the activation of NLRP1 inflammasome, reducing cell apoptosis and improving motor function of hind limbs.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省衛(wèi)生計(jì)生青年科研項(xiàng)目(2017-1-59)1 通信作者
更新日期/Last Update: 2020-04-15