80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]林文韜 王武煉 肖莉莉 張怡元.基于GEO數(shù)據(jù)庫發(fā)現(xiàn)骨肉瘤關鍵基因GMFG及其生物學功能[J].福建醫(yī)藥雜志,2020,42(02):112-115.
　LIN Wentao,WANG Wulian,XIAO Lili,et al.Identification of the key gene GMFG and its biological function in osteosarcoma based on GEO database[J].FUJIAN MEDICAL JOURNAL,2020,42(02):112-115.
點擊復制

基于GEO數(shù)據(jù)庫發(fā)現(xiàn)骨肉瘤關鍵基因GMFG及其生物學功能()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年02期
頁碼:
112-115
欄目:
基礎研究
出版日期:
2020-04-15

文章信息/Info

Title:
Identification of the key gene GMFG and its biological function in osteosarcoma based on GEO database
文章編號:
1002-2600(2020)02-0112-04
作者:
林文韜 王武煉 肖莉莉 張怡元
廈門大學附屬福州第二醫(yī)院骨科(福州 350007)
Author(s):
LIN Wentao;  WANG Wulian;  XIAO Lili;  ZHANG Yiyuan.
Department of Orthopedics, Fuzhou Second Hospital affiliated to Xiamen University, Fuzhou, Fuian 350007, China
關鍵詞:
骨肉瘤;  GMFG;  生物信息學
Keywords:
osteosarcoma;  GMFG;  bioinformatics
分類號:
R738.1
文獻標志碼:
A
摘要:
目的 基于生物信息學方法篩選骨肉瘤(OS)的關鍵基因及功能預測。方法 檢索GEO 數(shù)據(jù)庫OS數(shù)據(jù)集(GSE14359),使用GEO2R篩選差異基因(DEGs),構建DEGs的蛋白質-蛋白質相互作用網(wǎng)絡(PPI)以及Cytoscape可視化分析確定關鍵基因。并進一步下載關鍵基因共表達基因,基因本體(GO)和(京都基因與基因組百科全書)KEGG分析預測其可能的生物功能。結果 通過對原發(fā)OS組織、OS肺轉移組織和原代成骨細胞之間的差異性和維恩圖分析,獲得385個交集基因。進而使用蛋白質-蛋白質相互作用(PPI)和 Cytoscape 從交集基因中篩選出關鍵基因GMFG。 GMFG共表達基因主要參與中性粒細胞激活、中性粒細相關的免疫反應等過程,并顯著富集于核糖體細胞處理過程、白細胞跨膜轉運過程等通路。結論 GMFG作為OS關鍵基因,在OS進展中發(fā)揮促癌作用,可能是OS的可靠的診療靶點。
Abstract:
Objective To screen the key genes and functional prediction of osteosarcoma(OS)based on bioinformatics.Methods The OS dataset(GSE14359)from GEO database were retrieved, and GEO2R to screen the differential genes(DEGs)were used.We further constructed the protein and protein interaction network(PPI)of DEG, and identified the key gene through the visualized analysis of Cytoscape.The coexpression genes of key gene were downloaded, and the possible biological function was predicted by GO and KEGG analysis.Results By difference analysis and Venn diagram between conventional osteosarcoma tissue, osteosarcoma lung metastasis tissue and non-neoplastic primary osteoblast cells, we obtained 385 intersection genes.Then, PPI and Cytoscape were used to screen the key gene(GMFG).The co-expression genes of GMFG were mainly involved in neutrophil activation, neutrophil activation involved in immune response, and were significantly enriched in ribosome cell processing, leu-kocyte transendothelial migration.Conclusion As a key gene of OS, GMFG plays a role in promoting carcinogenesis in the progression of OS, which may be a reliable diagnosis and treatment target of OS.

參考文獻/References:

[1] Lindsey B A, Markel J E, Kleinerman E S.Osteosarcoma Overview[J].Rheumatol Ther, 2017, 4(1):25-43.
[2] Chen Z, Wu H, Wang G H, et al.Identification of potential candidate genes for hypertensive nephropathy based on gene expression profile[J].Bmc Nephrology, 2016, 17(1):149.
[3] Gandhi M, Smith B A, Bovellan M, et al.GMF is a cofilin homolog that binds Arp2/3 complex to stimulate filament debranching and Inhibit actin nucleation[J].Curr Biol, 2010, 20(9):861-867.
[4] Lippert D N.Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes[J].Bmc Immunology, 2012, 13(10):21.
[5] Aerbajinai W, Liu L, Chin K, et al.Glia maturation factor-γ mediates neutrophil chemotaxis[J].Journal of Leukocyte Biology, 2011, 90(3):529-538.
[6] Takeshi O, Yuki K, Yuichi A, et al.COXPRESdb v7: a gene coexpression database for 11 animal species supported by 23 coexpression platforms for technical evaluation and evolutionary inference[J].Nucleic Acids Research, 2019, 47(D1):D55-D62.
[7] Mahlam?ki E H, Kauraniemi P, Monni O, et al.High-resolution genomic and expression profiling reveals 105 putative amplification target genes in pancreatic cancer[J].Neoplasia, 2004, 6(5):432-439.
[8] Riina K, Kimmo S, Azorsa D O, et al.Intersex-like(IXL)is a cell survival regulator in pancreatic cancer with 19q13 amplification[J].Cancer Research, 2007, 67(5):1943-1949.
[9] Li Y L, Ye F, Cheng X D, et al.Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer[J].European Journal of Cancer, 2010, 46(11):2104-2118.
[10] Wang H, Chen Z, Chang H, et al.Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro[J].Oncolog y Reports, 2017, 37(2):929-936.
[11] Szczerba B M, Castro-Giner F, Vetter M, et al.Neutrophils escort circulating tumour cells to enable cell cycle progression[J].Nature, 2019, 566(7745):553-557.

相似文獻/References:

[1]肖莉莉 劉 暉 賈春鋒.基于核酸適配體的微流控芯片的構建及對循環(huán)腫瘤細胞篩選性能的測定[J].福建醫(yī)藥雜志,2020,42(02):119.
[2]陳康堯,顧恩毅,蔡碰德,等.H1受體拮抗劑異丙嗪對順鉑治療骨肉瘤的影響及機制[J].福建醫(yī)藥雜志,2021,43(04):132.
　CHEN Kangyao,GU Enyi,CAI Pengde,et al.Efficacy and mechanism of H1 receptor antagonist promethazine on osteosarcoma treated with cisplatin[J].FUJIAN MEDICAL JOURNAL,2021,43(02):132.
[3]鐘志輝,林焱斌,莊 研,等.骨肉瘤組織ACTN4和CD133的表達及其影響因素[J].福建醫(yī)藥雜志,2021,43(05):117.
　ZHONG Zhihui,LIN Yanbin,ZHUANG Yan,et al.Expression and interfering factors of ACTN4 and CD133 in osteosarcoma[J].FUJIAN MEDICAL JOURNAL,2021,43(02):117.
[4]林東澤.shRNA沉默c-myc基因對MG-63骨肉瘤細胞c-myc/PD-L1軸的影響[J].福建醫(yī)藥雜志,2022,44(05):133.
[5]周子杰,張家豪,姜吉霖,等.miR-586通過SFRP1調控骨肉瘤細胞增殖、遷移、侵襲的機制研究[J].福建醫(yī)藥雜志,2024,46(01):115.[doi:10.20148/j.fmj.2024.01.032]

備注/Memo

備注/Memo:
基金項目:2019年福建省衛(wèi)生健康青年科研項目(2019-1-84)
更新日期/Last Update: 2020-04-15