80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]李 敏 姜 鋒 魏瀟琪 王小易 林燕清 鄭煒平.Kisspeptin對(duì)胰島β細(xì)胞的凋亡影響及作用機(jī)制研究[J].福建醫(yī)藥雜志,2020,42(01):125-127.
　LI Min,JIANG Feng,WEI Xiaoqi,et al.Study on the effect and mechanism of kisspeptin on apoptosis of islet β cells[J].FUJIAN MEDICAL JOURNAL,2020,42(01):125-127.
點(diǎn)擊復(fù)制

Kisspeptin對(duì)胰島β細(xì)胞的凋亡影響及作用機(jī)制研究()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年01期
頁(yè)碼:
125-127
欄目:
基礎(chǔ)研究
出版日期:
2020-03-30

文章信息/Info

Title:
Study on the effect and mechanism of kisspeptin on apoptosis of islet β cells
文章編號(hào):
1002-2600(2020)01-0125-03
作者:
李 敏 姜 鋒1 魏瀟琪 王小易 林燕清 鄭煒平
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院老年科(福州 350001)
Author(s):
LI Min;  JIANG Feng;  WEI Xiaoqi;  WANG Xiaoyi;  LIN Yanqing;  ZHENG Weiping.
Department of Geriatric Medicine, Fujian Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University, Fuzhou,Fujian 350001, China
關(guān)鍵詞:
Kisspeptin;  ERK1/2;  糖尿病;  凋亡
Keywords:
Kisspeptin;  ERK1/2;  diabetes;  apoptosis
分類號(hào):
R-332; R587
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 研究Kisspeptin對(duì)胰島β細(xì)胞的凋亡影響及可能的作用機(jī)制。方法 取16只SD大鼠胰腺組織分為4組:1)對(duì)照組:在20%葡萄糖生理鹽水中培養(yǎng); 2)Kp組:在20%葡萄糖生理鹽水培養(yǎng)液中加入1 μmol/L Kisspeptin; 3)PD組:在20%葡萄糖生理鹽水培養(yǎng)液中加入50 μmol/L ERK1/2信號(hào)通路抑制劑PD98059; 4)Kp+PD組:在20%葡萄糖生理鹽水培養(yǎng)液中加入1 μmol/L Kisspeptin 和50 μmol/L PD98059。各組在恒溫CO2培養(yǎng)箱培養(yǎng)48 h后制作TUNEL染色切片,每組隨機(jī)取3張切片采用醫(yī)學(xué)圖像分析系統(tǒng)IPPS 6.0軟件計(jì)算每個(gè)胰島組織β細(xì)胞凋亡指數(shù),比較每組平均β細(xì)胞凋亡指數(shù)差異。結(jié)果 與對(duì)照組 [(4.08±0.42)%]、PD組[(4.10±0.42)%]、Kp+PD組[(4.11±0.47)%] 凋亡指數(shù)相比較,Kp組[(3.11±0.46)%]明顯下降(P均<0.01); PD組與對(duì)照組、Kp+PD組與對(duì)照組、Kp+PD組與PD組之間比較的差異均無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論 Kisspeptin對(duì)β細(xì)胞凋亡具有抑制作用, ERK1/2信號(hào)傳導(dǎo)通路可能是其主要的作用通路之一。
Abstract:
Objective To study the effect of Kisspeptin on the apoptosis of islet β cells and its possible mechanism.Methods Pancreatic tissues of 16 SD rats were divided into four groups: 1)control group: cultured in 20% glucose saline; 2)Kp group: 1 μmol/L Kisspeptin was added to 20% glucose saline solutions; 3)PD group: 50 μmol/L PD98059 was added to 20% glucose saline solution; 4): Kp+PD group: 1 μmol/L Kisspeptin and 50 μmol/L PD98059 were added to 20% glucose saline solution.TUNEL staining sections were prepared in a constant temperature CO2 incubator for 48 hours.Three sections were randomly selected from each group.The β-cell apoptosis index of each islet tissue was calculated by the medical image analysis system IPPS 6.0 software and the average β-cell apoptosis index of each group was compared.Results Compared with the control group [(4.08±0.42)%], the PD group[(4.10±0.42)%], and the Kp+PD group [(4.11±0.47)%], the average apoptotic index of the Kp group[(3.11±0.46)%] was significantly decreased(P<0.01 for each).There was no significant difference between PD group and control group, Kp+PD group and control group, Kp+PD group and PD group(P>0.05).Conclusion Kisspeptin has an inhibitory effect on β-cell apoptosis, and ERK1/2 signaling pathway may be one of the main pathways.

參考文獻(xiàn)/References:

[1] 中華醫(yī)學(xué)會(huì)糖尿病學(xué)分會(huì).中國(guó)2型糖尿病防治指南(2017年版)[J]. 中華糖尿病雜志,2018, 10(1): 4-67.
[2] Bowe J E, King A J, Kinsey-Jones J S, et al.Kisspeptin stimulation of insulin secretion: mechanisms of action in mouse islets and rats[J]. Diabetologia, 2009, 52(5): 855-862.
[3] Rahier J, Guiot Y, Goebbels R M, et al.Pancreatic beta-cell mass in European subjects with type 2 diabetes[J]. Diabetes Obes Metab, 2008, 10(Suppl 4): 32-42.
[4] Carlsson P O, Palm F, Andersson A, et al.Markedly decreased oxygen tension in transplanted rat pancreatic islets irrespective of the implantation site[J]. Diabetes, 2001, 50(3): 489-495.
[5] Dor Y, Brown J, Martinez O I, et al.Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation[J].Nature, 2004, 429(6987): 41-46.
[6] Brüning J C, Winnay J, Bonner-Weir S, et al.Development of a novel polygenic model of NIDDM in mice heterozygous for IR and IRS-1 null alleles[J]. Cell, 1997, 88(4): 561-572.
[7] Butler A E, Janson J, Bonner-Weir S,et al.Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes[J]. Diabetes, 2003, 52(1): 102-110.
[8] 溫俊平, 陳剛, 林麗香.Kisspeptin—連接能量與生殖的新紐帶[J]. 中華內(nèi)分泌代謝雜志, 2016, 32(7): 536-539.
[9] Seminara S B, Messager S, Chatzidaki E E, et al.The GPR54 gene as a regulator of puberty[J]. N Engl J Med, 2003, 349(17): 1614-1627.
[10] Kitsou-Mylona I, Burns C J, Squires P E, et al.A role for the extracellular calcium-sensing receptor in cell-cell communication in pancreatic islets of langerhans[J]. Cell Physiol Biochem, 2008, 22(5): 557-566.
[11] Hauge-Evans A C, Richardson C C, Milne H M, et al.A role for kisspeptin in islet function[J].Diabetologia,2006, 49(9): 2131-2135.
[12] Horiuchi Y, Nakatsu D, Kano F, et al.Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells[J]. Cell Signal, 2017, 38(10): 212-222.
[13] Smith J T, Acohido B V, Clifton D K, et al.KiSS-1 neurones are direct targets for leptin in the ob/ob mouse[J]. J Neuroendocrinol, 2006, 18(4): 298-303.

備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金項(xiàng)目(2016J01495)1 通信作者,Email: [email protected]
更新日期/Last Update: 2020-03-30