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[1]張紫冠 胡 森 陳文博 黃崢嶸 李衛(wèi)華.小G蛋白在心肌鈉通道蛋白轉(zhuǎn)運(yùn)中的作用[J].福建醫(yī)藥雜志,2018,40(06):114-117.
 ZHANG Ziguan,HU Sen,CHEN Wenbo,et al.Role of small GTP-binding proteins in cardiac voltage-gated sodium channel transport[J].FUJIAN MEDICAL JOURNAL,2018,40(06):114-117.
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小G蛋白在心肌鈉通道蛋白轉(zhuǎn)運(yùn)中的作用()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
40
期數(shù):
2018年06期
頁(yè)碼:
114-117
欄目:
基礎(chǔ)研究
出版日期:
2018-11-29

文章信息/Info

Title:
Role of small GTP-binding proteins in cardiac voltage-gated sodium channel transport
文章編號(hào):
1002-2600(2018)06-0114-05
作者:
張紫冠 胡 森 陳文博 黃崢嶸 李衛(wèi)華1
廈門(mén)大學(xué)附屬第一醫(yī)院心內(nèi)科 廈門(mén)大學(xué)醫(yī)學(xué)院心律失常與心臟電生理實(shí)驗(yàn)室(廈門(mén) 361003)
Author(s):
ZHANG Ziguan HU Sen CHEN Wenbo HUANG Zhengrong LI Weihua.
Arrhythmia and Cardiac Electrophysiology Laboratory,Medical College of Xiamen University; Department of Cardiovascular,the First Affiliated Hospital of Xiamen University, Xiamen,Fujian 361003,China
關(guān)鍵詞:
小G蛋白 Sar1 ARF1 SCN5A 基因 Nav1.5 蛋白
Keywords:
small GTPases Sar1 ARF1 SCN5A gene Nav1.5
分類(lèi)號(hào):
R363.1+4
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 研究小G蛋白Sar1和Arf1對(duì)鈉通道蛋白(Nav1.5)轉(zhuǎn)運(yùn)的調(diào)控作用。方法 以HEK293細(xì)胞作為宿主細(xì)胞,將人SCN5A基因轉(zhuǎn)染到HEK293細(xì)胞中,通過(guò)G418篩選穩(wěn)定表達(dá)SCN5A基因的細(xì)胞系; 為了檢測(cè)小G蛋白對(duì)Nav1.5的調(diào)節(jié)效應(yīng),野生型或突變型的小G蛋白Sar1和ARF1分別瞬轉(zhuǎn)到HEK293-Nav1.5細(xì)胞。利用免疫印跡對(duì)目標(biāo)蛋白進(jìn)行定量,應(yīng)用免疫熒光對(duì)細(xì)胞內(nèi)的目標(biāo)蛋白進(jìn)行定位。結(jié)果 過(guò)表達(dá)突變的Sar1減少了細(xì)胞膜上Nav1.5的表達(dá)和抑制了Nav1.5轉(zhuǎn)運(yùn)到細(xì)胞膜上。另外,過(guò)表達(dá)ARF1對(duì)于Nav1.5在細(xì)胞的表達(dá)及定位無(wú)明顯影響。結(jié)論 小G蛋白Sar1可能參與調(diào)節(jié)Nav1.5在細(xì)胞內(nèi)的運(yùn)輸。
Abstract:
Objective To investigate the role of small GTPases Sar1 and Arf1 in the transcription of Nav1.5 protein. Methods In this experiment,we used HEK 293 cells as the host cells to co-express Nav1.5 with the wild-type(WT)or mutant form of the small GTPases Sar1 and Arf1. Then in order to check the effect that small GTPase had on Nav1.5 regulation, two techniques were used to analyze the results. Western blot was performed to measure the expression level of Nav1.5, and Confocal microscopy was used to visualize the cellular localization of Nav1.5. Results We found that mutant forms of Sar1 decreased Nav1.5 expression level significantly and inhibited its localization at the cell membrane. On the other hand, Arf1 only showed small effect on the expression level and localization of Nav1.5. Conclusion This study provides evidences that Nav1.5 intracellular transport is regulated by small GTPases Sar1 in particular.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金資助項(xiàng)目(2015J01563) 1 通信作者,Email:[email protected]
更新日期/Last Update: 2018-11-29