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[1]謝文焌,李華水,林強(qiáng).miR-149-3p通過(guò)調(diào)控NIS促進(jìn)甲狀腺乳頭狀癌增殖和遷移[J].福建醫(yī)藥雜志,2024,46(04):94-99.[doi:10.20148/j.fmj.2024.04.028]
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miR-149-3p通過(guò)調(diào)控NIS促進(jìn)甲狀腺乳頭狀癌增殖和遷移()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年04期
頁(yè)碼:
94-99
欄目:
基礎(chǔ)研究
出版日期:
2024-08-15

文章信息/Info

文章編號(hào):
1002-2600(2024)04-0094-06
作者:
謝文焌李華水林強(qiáng)
福建省立醫(yī)院甲狀腺疝外科,福州 350001
關(guān)鍵詞:
甲狀腺腫瘤 miR-149-3p NIS 增殖活性 遷移
分類號(hào):
R736.1
DOI:
10.20148/j.fmj.2024.04.028
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 探討miR-149通過(guò)調(diào)控鈉/碘共同轉(zhuǎn)運(yùn)體(Sodium/Iodide Sympoter,NIS)調(diào)控甲狀腺乳頭狀癌(Papillary Thyroid Carcinoma,PTC)細(xì)胞增殖和遷移能力。方法 對(duì)選取的GSE113629轉(zhuǎn)錄組數(shù)據(jù)進(jìn)行數(shù)據(jù)處理,篩選其中表達(dá)差異較為明顯且在PTC組織中表達(dá)明顯增高的MicroRNA(miRNA)作為研究分子。通過(guò)在線數(shù)據(jù)庫(kù)預(yù)測(cè)目標(biāo)miRNA的下游靶基因,隨后檢測(cè)調(diào)控目標(biāo)miRNA表達(dá)后靶基因變化情況并觀察靶基因水平改變對(duì)甲狀腺癌細(xì)胞生物學(xué)行為的影響。結(jié)果 在GSE112629數(shù)據(jù)集中,研究發(fā)現(xiàn)miR-149-3p在PTC組織中高表達(dá)。通過(guò)對(duì)miR-149-3p預(yù)測(cè)靶基因進(jìn)行維恩分析,我們發(fā)現(xiàn)NIS是miR-149-3p的重要靶基因。通過(guò)分析GEPIA和UALCAN數(shù)據(jù)庫(kù),我們發(fā)現(xiàn)NIS在PTC樣本中明顯低表達(dá),同時(shí)發(fā)現(xiàn)PTC的淋巴結(jié)轉(zhuǎn)移率越高、分期級(jí)別越高則NIS表達(dá)量越低,并且NIS表達(dá)量與患者無(wú)病生存期(Disease-Free Survival,DFS)呈正相關(guān)。在多種PTC細(xì)胞系中miR-149-3p表達(dá)水平均高于正常甲狀腺細(xì)胞系,抑制PTC細(xì)胞內(nèi)miR-149-3p表達(dá)后細(xì)胞內(nèi)NIS表達(dá)水平明顯升高。當(dāng)敲低miR-149-3p時(shí),細(xì)胞的增殖活性和遷移能力受到明顯抑制。結(jié)論 PTC中miR-149 -3p高表達(dá),并且miR-149-3p 能夠通過(guò)抑制NIS表達(dá)明顯增強(qiáng)PTC的增殖活性和遷移能力。

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省衛(wèi)生健康委青年科研基金(2020QNA007)
通信作者:李華水,Email: [email protected]
更新日期/Last Update: 2024-08-15