80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]林濱,蔡沓栗,何小蓉,等.基于體外微量反應評價某些甾醇類化合物對5α還原酶的抑制作用[J].福建醫(yī)藥雜志,2024,46(04):91-94.[doi:10.20148/j.fmj.2024.04.027]
　LIN Bin,CAI Dali,HE Xiaorong,et al.Evaluation of the inhibitory effects of certain sterol compounds on 5 α-reductase based on in vitro micro reactions[J].FUJIAN MEDICAL JOURNAL,2024,46(04):91-94.[doi:10.20148/j.fmj.2024.04.027]
點擊復制

基于體外微量反應評價某些甾醇類化合物對5α還原酶的抑制作用()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年04期
頁碼:
91-94
欄目:
基礎研究
出版日期:
2024-08-15

文章信息/Info

Title:
Evaluation of the inhibitory effects of certain sterol compounds on 5 α-reductase based on in vitro micro reactions
文章編號:
1002-2600(2024)04-0091-04
作者:
林濱; 蔡沓栗; 何小蓉; 何宇; 江舉
福州市皮膚病防治院,福州 350025
Author(s):
LIN Bin; CAI Dali; HE Xiaorong; HE Yu; JIANG Ju
Dermatology Hospital of FuZhou,Fuzhou,Fujian 350025,China
關(guān)鍵詞:
5α還原酶;  非那雄胺;  體外;  脫發(fā)
Keywords:
5 α-reductase;  finasteride;  in vitro;  hair loss
分類號:
R914
DOI:
10.20148/j.fmj.2024.04.027
文獻標志碼:
B
摘要:
目的 基于體外微量反應,體外篩選有效的5α還原酶抑制物。方法 從SD大鼠肝臟中獲得5α還原酶的粗酶,建立體外微量反應體系,通過反應前后睪酮量的變化來評價受試甾醇類化合物對5α還原酶的抑制作用。結(jié)果 在反應體系中加入二甲基亞砜(DMSO),解決非那雄胺及受試甾醇類化合物在水相中溶解度低等的問題,確保微量反應有效進行,從而得到可靠的篩選結(jié)果。結(jié)論 羊毛甾醇在體外具有較好的5α還原酶抑制效果,或許可以成為新型的5α還原酶抑制物。
Abstract:
Objective To screen effective inhibitors of 5 α-reductase in vitro,based on in vitro micro reactions. Methods The crude enzyme of 5 α-reductase was obtained from the liver of SD rats,and then established a micro reaction system in vitro. The inhibitory effect of the tested sterol compounds on 5 α-reductase was evaluated by the changes in testosterone levels before and after the reaction. Results The addition of dimethyl sulfoxide(DMSO)to the reaction system solved the problem of low solubility of finasteride and tested sterol compounds in the aqueous phase,ensuring effective trace reactions and obtaining reliable screening results. Conclusion Lanosterol has a good inhibitory effect on 5 α-reductase in vitro,and may become a new type of 5 α-reductase inhibitor.

參考文獻/References:

[1] 鄭瑞蓉,歐陽倩兮,劉子瑤.天然5α-還原酶抑制劑用于改善良性前列腺增生的研究進展[J].中國中藥雜志,2024,49(4): 858-867.
[2] 崔心禹,崔心禹,夏琛,等.油茶葉提取物的5α-還原酶抑制活性及化學成分分析[J].食品工業(yè)科技,2021,42(19): 97-105.
[3] 李慕春,張顯文,王苗.12種植物的提取物對5α-還原酶的抑制活性研究[J].中南藥學,2017,15(11): 1536-1540.
[4] LOFTSSON T,HREINSDóTTIR D.Determination of aqueous solubility by heating and equilibration: a technical note[J].AAPS Pharm Sci Tech,2006,7(1): 29-32.
[5] ZHANG R W,ZHANG X Q.Inhibitory activities of some traditional Chinese herbs against testosterone 5α-reductase and effects of cacumen platycladi on hair re-growth in testosterone-treated mice[J].J Ethnopharmacol,2016(177):1-9.

備注/Memo

備注/Memo:
基金項目:福州市科學技術(shù)局醫(yī)療衛(wèi)生項目(2022-S-070); 福州市“十四五”臨床專科培育建設項目整形外科(20220303)
通信作者:江 舉,Email:[email protected]
更新日期/Last Update: 2024-08-15