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[1]陳康堯.自組裝脂質(zhì)體納米載體對(duì)骨肉瘤細(xì)胞Saos-2靶向遞送及凋亡的影響[J].福建醫(yī)藥雜志,2024,46(03):103-106.[doi:10.20148/j.fmj.2024.03.032]
　CHEN Kangyao.Study on targeted delivery and apoptosis effects of self-assembled liposome nanocarriers on osteosarcoma cells Saos-2[J].FUJIAN MEDICAL JOURNAL,2024,46(03):103-106.[doi:10.20148/j.fmj.2024.03.032]
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自組裝脂質(zhì)體納米載體對(duì)骨肉瘤細(xì)胞Saos-2靶向遞送及凋亡的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年03期
頁(yè)碼:
103-106
欄目:
基礎(chǔ)研究
出版日期:
2024-06-15

文章信息/Info

Title:
Study on targeted delivery and apoptosis effects of self-assembled liposome nanocarriers on osteosarcoma cells Saos-2
文章編號(hào):
1002-2600(2024)03-0103-04
作者:
陳康堯
福建省福州市第二總醫(yī)院骨科,福州 350007
Author(s):
CHEN Kangyao
Department of Orthopaedics,Fuzhou Second General Hospital,Fuzhou,Fujian 350007,China
關(guān)鍵詞:
脂質(zhì)體納米載體;  阿霉素;  骨肉瘤細(xì)胞Saos-2;  細(xì)胞靶向;  細(xì)胞凋亡
Keywords:
liposome nanocarriers;  doxorubicin;  osteosarcoma cells saos-2;  cell targeting;  apoptosis
分類(lèi)號(hào):
R738.1
DOI:
10.20148/j.fmj.2024.03.032
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 以天然脂肪酸月桂酸和硬脂酸為骨架,利用它們?cè)诙谆柞０?dimethylformamide,DMF)中的自組裝構(gòu)建了一種脂質(zhì)體納米載體并負(fù)載阿霉素,對(duì)其靶向人骨肉瘤細(xì)胞Saos-2及凋亡情況進(jìn)行考察。方法 采用自組裝制備了負(fù)載阿霉素的脂質(zhì)體納米載體,采用激光共聚焦考察了其靶向人骨肉瘤細(xì)胞Saos-2能力,采用Annexin V-FITC染色技術(shù)考察了脂質(zhì)體納米載體對(duì)人骨肉瘤細(xì)胞Saos-2凋亡的影響。結(jié)果 自組裝脂質(zhì)體納米載體與人骨肉瘤細(xì)胞Saos-2孵育48 h時(shí),熒光強(qiáng)度最高,綠色熒光的自組裝脂質(zhì)體納米載體會(huì)圍繞在骨肉瘤細(xì)胞Saos-2周?chē)?相對(duì)于同劑量DMF的對(duì)照組,自組裝脂質(zhì)體納米載體組的細(xì)胞無(wú)論是早期凋亡還是晚期凋亡的比例均有所增加。結(jié)論 負(fù)載阿霉素的脂質(zhì)體納米載體能準(zhǔn)確地靶向人骨肉瘤細(xì)胞Saos-2,并加速人骨肉瘤細(xì)胞Saos-2的凋亡。
Abstract:
Objective Using natural fatty acids lauric acid and stearic acid as the backbone, a liposome nanocarrier was constructed through their self-assembly in dimethylformamide(DMF)and loaded with doxorubicin.Its targeting ability to human osteosarcoma cells Saos-2 and apoptosis were investigated.Methods Doxorubicin-loaded liposome nanocarriers were prepared by self-assembly.The targeting ability of the nanocarriers to human osteosarcoma cells Saos-2 was examined using confocal laser scanning microscopy.The effect of liposome nanocarriers on the apoptosis of human osteosarcoma cells Saos-2 was investigated using Annexin V-FITC staining technique.Results When the self-assembled liposome nanocarriers were incubated with human osteosarcoma cells Saos-2 for 48 hours, the fluorescence intensity was the highest.The green fluorescent self-assembled liposome nanocarriers surrounded the osteosarcoma cells Saos-2.Compared to the control group with the same dose of DMF, both early and late apoptotic cell proportions increased in the self-assembled liposome nanocarrier group.Conclusion Doxorubicin-loaded liposome nanocarriers can accurately target human osteosarcoma cells Saos-2 and accelerate their apoptosis.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福州市科技計(jì)劃項(xiàng)目(2021-S-166); 福建省創(chuàng)傷骨科急救與康復(fù)臨床醫(yī)學(xué)研究中心項(xiàng)目(2020Y2014)
更新日期/Last Update: 2024-06-15