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[1]汪家航,林明強(qiáng),張捷平.NCAPG對(duì)大腸癌細(xì)胞HCT116增殖和凋亡的影響[J].福建醫(yī)藥雜志,2024,46(03):94-99.[doi:10.20148/j.fmj.2024.03.030]
　WANG Jiahang,LIN Mingqiang,ZHANG Jieping.Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116[J].FUJIAN MEDICAL JOURNAL,2024,46(03):94-99.[doi:10.20148/j.fmj.2024.03.030]
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NCAPG對(duì)大腸癌細(xì)胞HCT116增殖和凋亡的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年03期
頁(yè)碼:
94-99
欄目:
基礎(chǔ)研究
出版日期:
2024-06-15

文章信息/Info

Title:
Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116
文章編號(hào):
1002-2600(2024)03-0094-06
作者:
汪家航1; 林明強(qiáng)1; 張捷平2
1 福州市第二總醫(yī)院胃腸外科,福州 350025; 2 福建中醫(yī)藥大學(xué),福州 350122
Author(s):
WANG Jiahang1;  LIN Mingqiang1;  ZHANG Jieping2
1 Department of Gastrointestinal Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian 350025, China; 2 Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350000, China
關(guān)鍵詞:
非染色體結(jié)構(gòu)維持蛋白復(fù)合體I G亞基;  大腸癌;  細(xì)胞生長(zhǎng);  凋亡
Keywords:
NCAPG;  colorectal cancer;  cell growth;  apoptosis
分類(lèi)號(hào):
R735.3+4
DOI:
10.20148/j.fmj.2024.03.030
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討非染色體結(jié)構(gòu)維持蛋白復(fù)合體I G亞基(Non-SMC condensin I complex subunit G,NCAPG)對(duì)大腸癌細(xì)胞生長(zhǎng)的作用和對(duì)增殖凋亡的影響。方法 敲減NCAPG的小干擾RNA(si-NCAPG)轉(zhuǎn)染人大腸癌細(xì)胞株HCT116,Western blot檢測(cè)si-NCAPG對(duì)大腸癌細(xì)胞中NCAPG蛋白表達(dá)的影響; 通過(guò)觀察細(xì)胞形態(tài)、細(xì)胞活力評(píng)價(jià)si-NCAPG對(duì)大腸癌細(xì)胞生長(zhǎng)的影響,并篩選效果最好的序列用于后續(xù)實(shí)驗(yàn)。通過(guò)克隆形成、細(xì)胞周期和凋亡、Western blot實(shí)驗(yàn)評(píng)價(jià)si-NCAPG對(duì)大腸癌細(xì)胞生長(zhǎng)、周期和凋亡的影響。結(jié)果 si-NCAPG顯著降低大腸癌細(xì)胞中NCAPG的蛋白表達(dá)(P<0.05),抑制大腸癌細(xì)胞數(shù)目和細(xì)胞活力(P<0.05),使細(xì)胞阻滯在G2/M期(P<0.05),并促進(jìn)細(xì)胞凋亡(P<0.05)。敲減NCAPG降低Cyclin B1、CDK1和Bcl-2蛋白表達(dá)水平和升高BAX的蛋白表達(dá)水平(P<0.05)。結(jié)論 敲減NCAPG通過(guò)阻滯細(xì)胞在G2/M期和促進(jìn)細(xì)胞凋亡,達(dá)到抑制大腸癌細(xì)胞生長(zhǎng)的作用。
Abstract:
Objective To investigate the effect and mechanism of Non-SMC condensin I complex subunit G(NCAPG)on the growth,proliferation and apoptosis of colorectal cancer cells(CRC).Methods siRNA-NCAPG was transfected into human colorectal cancer cell line HCT116 cells.The effect of si-NCAPG on the expression of NCAPG in HCT116 cells was detected by Western blot.The effect of si-NCAPG on the growth of HCT116 cells was evaluated by observing cell morphology and cell viability, and the best sequences were screened for subsequent experiments.The effects of si-NCAPG on the cell growth, cycle and apoptosis of HCT116 cells were evaluated by clonal formation, cell cycle, apoptosis, and western blot.Results si-NCAPG significantly decreased the expression of NCAPG protein level(P<0.05), inhibited the cell number and cell viability(P<0.05), induced cell cycle arrest in G2/M phase(P<0.05)and promoted cell apoptosis in HCT116 cells(P<0.05).Knocking down NCAPG reduced expression levels of Cyclin B1, CDK1 and Bcl-2 protein and increased expression levels of BAX protein.Conclusion si-NCAPG significantly inhibits the growth of CRC by inducing cell cycle arrest at G2/M phase and promoting cell apoptosis.

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備注/Memo

備注/Memo:
基金項(xiàng)目:2021年福州市衛(wèi)生健康中青年科學(xué)研究項(xiàng)目(2021-S-wq19); 福建省創(chuàng)傷骨科急救與康復(fù)臨床醫(yī)學(xué)研究中心項(xiàng)目(2020Y2014)
更新日期/Last Update: 2024-06-15