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[1]鄭述建,宋霖,嚴(yán)成,等.shRNA沉默circ_0089153對TPC-1甲狀腺癌細(xì)胞增殖、遷移及侵襲的影響[J].福建醫(yī)藥雜志,2024,46(03):90-93.[doi:10.20148/j.fmj.2024.03.029]
　ZHENG Shujian,SONG Lin,YAN Cheng,et al.Effect of shRNA silencing circ_0089153 on proliferation, migration and invasion of TPC-1 thyroid cancer cells[J].FUJIAN MEDICAL JOURNAL,2024,46(03):90-93.[doi:10.20148/j.fmj.2024.03.029]
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shRNA沉默circ_0089153對TPC-1甲狀腺癌細(xì)胞增殖、遷移及侵襲的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年03期
頁碼:
90-93
欄目:
基礎(chǔ)研究
出版日期:
2024-06-15

文章信息/Info

Title:
Effect of shRNA silencing circ_0089153 on proliferation, migration and invasion of TPC-1 thyroid cancer cells
文章編號:
1002-2600(2024)03-0090-04
作者:
鄭述建; 宋霖; 嚴(yán)成; 陳榕
福建省福州市第二總醫(yī)院甲乳外科,福州350007
Author(s):
ZHENG Shujian;  SONG Lin;  YAN Cheng;  CHEN Rong
Department of Thyroid and Breast Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian 350007, China
關(guān)鍵詞:
甲狀腺癌;  circ_0089153;  細(xì)胞增殖;  細(xì)胞遷移;  細(xì)胞侵襲
Keywords:
thyroid cancer;  circ_0089153;  cell proliferation;  cell migration;  cell invasion
分類號:
R73
DOI:
10.20148/j.fmj.2024.03.029
文獻標(biāo)志碼:
B
摘要:
目的 探討circ_0089153對TPC-1甲狀腺癌細(xì)胞增殖、遷移及侵襲的影響。方法 采用circ_0089153 shRNA沉默TPC-1細(xì)胞中的circ_0089153表達(dá)并分成空白對照組、陰性對照組及shRNA沉默組,通過RT-PCR及Western blot檢測不同組TPC-1細(xì)胞中circ_0089153 mRNA及蛋白的表達(dá),采用CCK-8法檢測不同組TPC-1細(xì)胞增殖,采用Transwell實驗檢測不同組細(xì)胞遷移和侵襲能力。結(jié)果 shRNA沉默組circ_0089153 mRNA及蛋白表達(dá)水平較空白對照組、陰性對照組低(P<0.05); 沉默hsa_circ_0089153后,TPC-1細(xì)胞增殖受到抑制; shRNA沉默組細(xì)胞增殖率、遷移和侵襲的細(xì)胞數(shù)較對照組低(P<0.05)。結(jié)論 circ_0089153與甲狀腺癌細(xì)胞增殖、遷移及侵襲密切相關(guān)。
Abstract:
Objective To investigate the effects of circ_0089153 on the proliferation, migration and invasion of TPC-1 thyroid cancer cells.Methods The expression of circ_0089153 in TPC-1 cells was silenced by circ_0089153 shRNA and divi-ded into blank control group, negative control group and shRNA silenced group.The expression of circ_0089153 mRNA and protein in TPC-1 cells in different groups was detected by RT-PCR and Western blot, the proliferation of TPC-1 cells in different groups was detected by CCK-8 method, and the migration and invasion ability of TPC-1 cells in different groups was detected by transwell assay.Results The mRNA and protein expression levels of circ_0089153 in shRNA silencing group were lower than those in blank control group and negative control group(P<0.05).After silencing hsa_circ_0089153, TPC-1 cell proliferation was inhibited.The number of cell proliferation, migration and invasion in shRNA silencing group was lower than that in control group(P<0.05).Conclusion circ_0089153 is closely related to the proliferation, migration and invasion of thyroid cancer cells.

參考文獻/References:

[1] SUNG H, FERLAY J, SIEGEL R L, et al.Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3):209-249.
[2] 姜帆,付振濤,鹿子龍,等.基于年齡-時期-隊列效應(yīng)模型分析2012-2022年山東省甲狀腺癌流行趨勢[J].中華預(yù)防醫(yī)學(xué)雜志,2024,58(1):56-64.
[3] SALZMAN J, GAWAD C, WANG P L, et al.Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types[J].PLS ONE,2012,7(2):e30733.
[4] 丁俊竹,孫玉雙,李姍姍.環(huán)狀RNA的研究進展[J]. 國際遺傳學(xué)雜志,2020,43(1): 27-29.
[5] LIU J, QIAO X, LIU J, et al.Identification of circ_0089153/miR-608/EGFR p53 axis in ameloblastoma via MAPK signaling pathway[J]. Oral Dis,2022,28(3):756-770.
[6] BRAY F, FERLAY J, SOERJOMATARAM I, et al.Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018,68(6):394-424.
[7] FIORE M, OLIVERI CONTI G, CALTABIANO R, et al.Role of emerging environmental risk factors in thyroid cancer: a brief review[J].Int J Environ Res Public Health,2019,16(7):1185.
[8] TABATABAEIAN H, PEILING YANG S, TAY Y.Non-coding RNAs:uncharted mediators of thyroid cancer pathogenesis[J].Cancers(Basel),2020,12(11):3264-3300.
[9] SHEN Z, ZHOU L, ZHANG C, et al.Reduction of circular RNA Foxo3 promotes prostate cancer progression and chemoresistance to docetaxel[J].Cancer Lett,2020,6(1):88-101.
[10] ZHOU G K, ZHANG G Y, YUAN Z N, et al.Has_circ_0008274 promotes cell proliferation and invasion involving AMPK/mTOR signaling pathway in papillary thyroid carcinoma[J].Eur Rev Med Pharmacol Sci,2018,22(24):8772-8780.
[11] CORK G K, THOMPSON J, SLAWSON C.Real talk: the inter-play between the mTOR, AMPK, and hexosamine biosynthetic pathways in cell signaling[J].Front Endocrinol(Lausanne),2018, 6(9):522.
[12] LIU F, ZHANG J, QIN L, et al.Circular RNA EIF6(Hsa_circ_0060060)sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation[J].Aging(Albany NY),2018,10(12):3806-3820.
[13] ALLINEN M, BEROUKHIM R, CAI L, et al.Molecular characterization of the tumor microenvironment in breast cancer[J].Cancer Cell,2004,6(1):17-32.
[14] GAO S L, FAN Y, LIU X D,et al.Circ_0089153 exacerbates breast cancer cells proliferation and metastasis via sponging miR-2467-3p/E2F6[J].Environmental Toxicology,2022, 37(6):1458-1471.
[15] 王偉剛,田保國,王艷,等.乳腺癌與甲狀腺疾病關(guān)系研究進展[J].腫瘤研究與臨床,2020,32(3): 206-209.

備注/Memo

備注/Memo:
基金項目:福建省自然科學(xué)基金資助項目(2022J011322)
更新日期/Last Update: 2024-06-15