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[1]黃建平,吳雪晶,鄭宇輝,等.GRWD1對(duì)胃癌細(xì)胞增殖和侵襲能力的影響[J].福建醫(yī)藥雜志,2024,46(03):5-8.[doi:10.20148/j.fmj.2024.03.002]
　HUANG Jianping,WU Xuejing,ZHENG Yuhui,et al.Effect of GRWD1 on the proliferation and invasion of gastric cancer cells[J].FUJIAN MEDICAL JOURNAL,2024,46(03):5-8.[doi:10.20148/j.fmj.2024.03.002]
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GRWD1對(duì)胃癌細(xì)胞增殖和侵襲能力的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年03期
頁(yè)碼:
5-8
欄目:
臨床研究
出版日期:
2024-06-15

文章信息/Info

Title:
Effect of GRWD1 on the proliferation and invasion of gastric cancer cells
文章編號(hào):
1002-2600(2024)03-0005-04
作者:
黃建平; 吳雪晶; 鄭宇輝; 李小燕; 施棟梁; 楊映紅
福建醫(yī)科大學(xué)附屬協(xié)和醫(yī)院病理科,福州 350001
Author(s):
HUANG Jianping; WU Xuejing; ZHENG Yuhui; LI Xiaoyan; SHI Dongliang; YANG Yinghong
Department of Pathology, the Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, China
關(guān)鍵詞:
GRWD1;  胃癌;  增殖;  侵襲
Keywords:
GRWD1;  gastric cancer;  proliferation;  invasion
分類號(hào):
R735.2
DOI:
10.20148/j.fmj.2024.03.002
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討富含谷氨酸的WD重復(fù)序列蛋白1(glutamate-rich WD40 repeat containing 1,GRWD1)蛋白表達(dá)水平對(duì)胃癌MKN-45細(xì)胞增殖和侵襲能力的影響。方法 分別構(gòu)建GRWD1過(guò)表達(dá)載體和設(shè)計(jì)siRNA并轉(zhuǎn)染胃癌MKN-45細(xì)胞,設(shè)立GRWD1過(guò)表達(dá)組及對(duì)照組、siRNA基因沉默組及對(duì)照組。采用CCK-8法測(cè)定各組細(xì)胞增殖差異,采用transwell檢測(cè)GRWD1過(guò)表達(dá)和基因沉默對(duì)胃癌細(xì)胞MKN-45侵襲能力的影響。結(jié)果 siRNA組轉(zhuǎn)染MKN-45細(xì)胞24 h后,細(xì)胞增殖水平和侵襲能力明顯受到抑制(P<0.01),過(guò)表達(dá)組轉(zhuǎn)染MKN-45細(xì)胞24 h后,細(xì)胞增殖水平和侵襲能力明顯增強(qiáng)(P<0.01)。結(jié)論 GRWD1與MKN-45胃癌細(xì)胞增殖和侵襲能力有關(guān),下調(diào)GRWD1可抑制MKN-45細(xì)胞增殖水平和侵襲能力。反之,上調(diào)GRWD1可提高M(jìn)KN-45細(xì)胞增殖水平和侵襲能力。
Abstract:
Objective To investigate the effect of GRWD1(Glutamate-rich WD40 repeat containing 1)on the proliferation and invasion ability of gastric cancer MKN-45 cells.Methods GRWD1 overexpression vector and siRNA were constructed and transfected into gastric cancer MKN-45 cells.GRWD1 overexpression group, siRNA group and control group were established.Cell proliferation levels in each group were measured using the CCK-8, and transwell was used to detect the effects of GRWD1 overexpression and gene silencing on the invasion ability of gastric cancer cell line MKN-45.Results The results showed that after transfection of MKN-45 cells with siRNA for 24 hours, the cell proliferation level and invasion ability were significantly inhibited(P<0.01), while after transfection of MKN-45 cells with overexpression vector for 24 hours, the cell proliferation level and invasion ability were significantly enhanced(P<0.01).Conclusion GRWD1 is related to the proliferation and invasion ability of MKN-45 gastric cancer cells, and downregulation of GRWD1 can inhibit the proliferation level and invasion ability of MKN-45 cells.On the contrary, upregulation of GRWD1 can improve the proliferation level and invasion ability of MKN-45 cells.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金(2022J01719)
通信作者:楊映紅,Email:[email protected]
更新日期/Last Update: 2024-06-15