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[1]黃建剛,沈菁,陳雯,等.長(zhǎng)鏈非編碼RNA AC004920.3在胃腺癌癌栓和細(xì)胞中的作用[J].福建醫(yī)藥雜志,2024,46(01):5-8.[doi:10.20148/j.fmj.2024.01.002]
　HUANG Jiangang,SHEN Jing,CHEN Wen,et al.Effects of long non-coding RNA AC004920.3 on cancer embolus and cells of gastric adenocarcinoma[J].FUJIAN MEDICAL JOURNAL,2024,46(01):5-8.[doi:10.20148/j.fmj.2024.01.002]
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長(zhǎng)鏈非編碼RNA AC004920.3在胃腺癌癌栓和細(xì)胞中的作用()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期數(shù):
2024年01期
頁(yè)碼:
5-8
欄目:
臨床研究
出版日期:
2024-02-15

文章信息/Info

Title:
Effects of long non-coding RNA AC004920.3 on cancer embolus and cells of gastric adenocarcinoma
文章編號(hào):
1002-2600（2024）01-0005-04
作者:
黃建剛1; 沈菁2; 陳雯2; 林有東2
1 福建省臨床檢驗(yàn)中心，福州 350001；2 福建省立醫(yī)院檢驗(yàn)科，福州 350001
Author(s):
HUANG Jiangang1;  SHEN Jing2;  CHEN Wen2;  LIN Youdong2
1 Fujian Provincial Center for Clinical Laboratory, Fuzhou, Fujian 350001, China; 2 Department of Clinical Laboratory Medicine, Fujian Provincial Hospital, Fuzhou, Fujian 350001, China
關(guān)鍵詞:
AC004920.3; 胃腺癌; 脈管癌栓; 細(xì)胞凋亡; 細(xì)胞增殖; 細(xì)胞遷移
Keywords:
AC004920.3;  gastric adenocarcinoma; vascular cancer thrombus; cell apoptosis; cell proliferation; cell migration
分類(lèi)號(hào):
R319；R737.3
DOI:
10.20148/j.fmj.2024.01.002
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的探尋AC004920.3在胃腺癌患者血清中的表達(dá)和細(xì)胞中的作用。方法收集胃腺癌患者血清標(biāo)本和病歷資料。PCR擴(kuò)增AC004920.3的表達(dá)。慢病毒過(guò)表達(dá)AC004920.3轉(zhuǎn)染AGS細(xì)胞后進(jìn)行凋亡、CCK增殖和劃痕實(shí)驗(yàn)。結(jié)果胃腺癌脈管癌栓患者血清AC004920.3表達(dá)ΔCt值12.06±3.33明顯高于無(wú)脈管癌栓ΔCt值的10.05±3.32，差異有統(tǒng)計(jì)學(xué)意義（P=0.003），與D-二聚體呈正相關(guān)（r=0.679,P<0.001）。AC004920.3過(guò)表達(dá)組AGS細(xì)胞早期凋亡率為（33.45±3.90）%，高于未轉(zhuǎn)染組[（12.13±1.54）%,P<0.001]和空載體組[（13.18±1.55）%，P<0.001]；CCK增殖實(shí)驗(yàn)AC004920.3過(guò)表達(dá)組的A值為2.89±0.17，低于未轉(zhuǎn)染組的3.20±0.21，差異有統(tǒng)計(jì)學(xué)意義（P=0.003）；也低于空載體組3.28±0.18，差異有統(tǒng)計(jì)學(xué)意義（P=0.001)；AC004920.3過(guò)表達(dá)組的劃痕面積（3.54±0.12）灰度值，明顯大于未轉(zhuǎn)染組和空載體組（2.43±0.34）灰度值，差異有統(tǒng)計(jì)學(xué)意義（P=0.001）；也大于空載體組（2.60±0.39）灰度值，差異有統(tǒng)計(jì)學(xué)意義（P=0.004）。結(jié)論胃腺癌脈管癌栓患者血清AC004920.3高表達(dá)，且與D-二聚體正相關(guān)。AC004920.3促進(jìn)AGS細(xì)胞凋亡以及抑制AGS細(xì)胞增殖和遷移。
Abstract:
ObjectiveTo investigate the expression of AC004920.3 in serum of gastric adenocarcinoma patients and its effect on cells.MethodsSerum samples and medical records were collected.The expression of AC004920.3 was amplified by PCR.After transfecting AGS cells with lentivirus overexpression of AC004920.3, apoptosis, CCK proliferation and scratches were performed.ResultsSerum AC004920.3 expression 12.06±3.33 was significantly lower in patients with gastric adenocarcinoma with venous embolus than that without venous embolus(10.05±3.32, P=0.003), and was positively correlated with D-dimer(r=0.679, P<0.001).The early apoptosis rate of AGS cells in AC004920.3 overexpression group(33.45±3.90)% was higher than that in untransfected group [(12.13±1.54）%,P<0.001] and transfection negative control group[(13.18±1.55）%，P<0.001].The proliferation of CCK showed that OD value of AC004920.3 overexpression group(2.89±0.17) was lower than that of untransfected group and transfection negative control group(3.20±0.21, P=0.003; 3.28±0.18, P=0.001). The scratch area of AC004920.3 overexpression group(3.54±0.12) was significantly larger than that of untransfected group and transfection negative control group(2.43±0.34, P=0.001; 2.60±0.39, P=0.004).ConclusionThe serum AC004920.3 expression in patients with gastric adenocarcinoma with venous embolus was lower than that without venous embolus, and was positively correlated with D- dimer.AC004920.3 promoted the apoptosis of AGS cells and inhibited the proliferation and migration of AGS cells.

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備注/Memo

備注/Memo:
基金項(xiàng)目：福建省衛(wèi)健委醫(yī)學(xué)創(chuàng)新課題(2018007)
通信作者：林有東，Email：[email protected]
更新日期/Last Update: 2024-02-15