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[1]林潔,廖心成,錢彬.和厚樸酚抑制IDO1表達改善老年小鼠術后認知功能障礙的實驗研究[J].福建醫(yī)藥雜志,2023,45(06):111-115.
 LIN Jie,LIAO Xincheng,QIAN Bin.Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice[J].FUJIAN MEDICAL JOURNAL,2023,45(06):111-115.
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和厚樸酚抑制IDO1表達改善老年小鼠術后認知功能障礙的實驗研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數:
2023年06期
頁碼:
111-115
欄目:
基礎研究
出版日期:
2023-12-15

文章信息/Info

Title:
Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice
文章編號:
1002-2600(2023)06-0111-05
作者:
林潔廖心成1錢彬2
福建中醫(yī)藥大學附屬人民醫(yī)院麻醉科(福州 350004)
Author(s):
LIN Jie LIAO Xincheng QIAN Bin
Department of Anesthesiology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
關鍵詞:
認知功能障礙 和厚樸酚 色氨酸代謝 吲哚胺23雙加氧酶1(IDO1)
Keywords:
cognitive dysfunction honokiol tryptophan metabolism indoleamine 23-dioxygenase 1
分類號:
R589
文獻標志碼:
A
摘要:
目的 探討和厚樸酚對術后認知功能障礙防治作用的機制。方法 將18月齡雄性C57BL/6小鼠隨機分為4組:Control組(溶劑預處理7 d,不進行手術)、HNK組(10 mg/kg和厚樸酚預處理7 d,不進行手術)、Surgery組(溶劑預處理7 d,脾切除術建模)和Surgery+HNK組(10 mg/kg和厚樸酚預處理7 d,脾切除術建模)。運用Morris水迷宮評估空間記憶功能,免疫熒光染色觀察海馬凋亡神經元比例,檢測海馬Bcl-2、Bax、活化Caspase-3以及吲哚胺2,3雙加氧酶1(indoleamine 2,3-dioxygenase 1, IDO1)表達水平,高效液相色譜檢測色氨酸毒性代謝產物含量。結果 與Surgery組相比,Surgery+HNK組水迷宮測試期第1、3、7天目標象限停留時間和平臺穿越次數增加; 術后第7天海馬凋亡神經元比例降低; 術后第1天Bcl-2表達增高、Bax表達水平降低、活化Caspase-3水平降低,IDO1表達降低; 色氨酸毒性代謝物降低。結論 和厚樸酚可通過抑制IDO1表達,調控色氨酸代謝,減輕海馬神經元損傷,改善小鼠術后認知功能障礙。
Abstract:
Objective To explore the mechanism by which honokiol improves postoperative cognitive dysfunction(POCD)in elderly mice.Methods The 18-month-old male C57BL/6J mice were randomly divided into four groups: control group, HNK group, surgery group and surgery+HNK group.We conducted splenectomy to establish a POCD animal model.The Morris water maze(MWM)test was performed to assess the hippocampus-related cognitive function.TdT-mediated dUTP nick end labeling(TUNEL)staining was used to assess the proportion of apoptotic neurons of the hippocampus at 24 h postope-ratively.The hippocampal levels of Bcl-2, Bax, cleaved caspase-3, and indoleamine 2,3-dioxygenase 1(IDO1)were evaluated using western blotting.The contents of tryptophan metabolites kynurenine, 3-hydroxykynurenine(3-HK), 3-hydroxyanthranilic acid(3-HAA), and quinolinic acid(QA)were also detected using high performance liquid chromatography(HPLC).Results Compared with the surgery group, the surgery+HNK group mice showed increased target quadrant residence time and platform crossing times on the 1st, 3rd, and 7th day of the MWM testing period; The proportion of apoptotic neurons of hippocampal decreased in the surgery+HNK group on postoperative day 7; Honokiol pretreatment increased the level of Bcl-2 and decreased the levels of Bax, cleaved caspase-3, and IDO1 in the hippocampus on postoperative day 1.The HPLC results showed that honokiol pretreatment also reduced the contents of kynurenine, 3-HK, 3-HAA and QA in the hippocampus on postoperative day 1.Conclusion Honokiol alleviates hippocampal neuronal damage and improves POCD in elderly mice by inhibiting IDO1 expression and regulating hippocampal tryptophan metabolism.

參考文獻/References:

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備注/Memo

備注/Memo:
基金項目:福建省中青年教師教育科研項目(JAT220114); 福建省自然科學基金(2022J01847)
1 福建中醫(yī)藥大學附屬第二人民醫(yī)院麻醉科; 2 通信作者
更新日期/Last Update: 2023-12-15