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[1]張良志,劉,洪,等.基于NLRP3/caspase-1通路探討針刀對兔膝骨關(guān)節(jié)炎軟骨細(xì)胞的影響[J].福建醫(yī)藥雜志,2023,45(05):87-90.
　ZHANG Liangzhi,LIU Hong,XIE Ziyi,et al.Effect of acupotomy on chondrocytes in rabbit knee osteoarthritis based on NLRP3/caspase-1 pathway[J].FUJIAN MEDICAL JOURNAL,2023,45(05):87-90.
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基于NLRP3/caspase-1通路探討針刀對兔膝骨關(guān)節(jié)炎軟骨細(xì)胞的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年05期
頁碼:
87-90
欄目:
基礎(chǔ)研究
出版日期:
2023-10-15

文章信息/Info

Title:
Effect of acupotomy on chondrocytes in rabbit knee osteoarthritis based on NLRP3/caspase-1 pathway
文章編號(hào):
1002-2600(2023)05-0087-04
作者:
張良志1; 2; 劉; 洪1; 2; 謝梓毅3; 李陽陽3; 余文英3; 劉晶1; 修忠標(biāo)1; 2; 3; 4
福建中醫(yī)藥大學(xué)附屬人民醫(yī)院骨傷二科(福州 350004)
Author(s):
ZHANG Liangzhi;  LIU Hong;  XIE Ziyi;  LI Yangyang;  YU Wenying;  LIU Jin;  XIU Zongbiao
Second Department of Orthopedics-traumatology, The People's Hospital Affiliated of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
關(guān)鍵詞:
膝骨關(guān)節(jié)炎;  針刀;  NLRP3/Caspase-1通路;  軟骨細(xì)胞焦亡
Keywords:
kneeosteoarthritis(KOA);  acupotomy;  NLRP3/Caspase-1 pathway;  chondrocyte pyroptosis
分類號(hào):
R681.3
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 觀察針刀對膝骨關(guān)節(jié)炎(KOA)兔軟骨NLRP3/Caspase-1通路的調(diào)控作用及軟骨細(xì)胞焦亡的影響。方法 選用24只新西蘭兔雄兔,年齡均為6個(gè)月。使用隨機(jī)數(shù)字表法將其分為3組,分別為空白組、模型組、針刀組,每組各8只。造模方法采用改良Videman法進(jìn)行固定,造模時(shí)間為6周,空白組不予固定。針刀組選取經(jīng)筋病灶點(diǎn)行針刀松解治療,每周1 次,共治療3 次; 空白組和模型組正常飼養(yǎng),行同樣抓捕行為。采用HE染色觀察軟骨形態(tài)學(xué)改變; 并檢測軟骨Caspase-1、NLRP3、ASC、IL-1βmRNA和蛋白的表達(dá)。結(jié)果 空白組軟骨細(xì)胞均勻地分布在軟骨基質(zhì)中,表面相對光滑,層次結(jié)構(gòu)較為清晰,潮線清晰完整。模型組軟骨細(xì)胞數(shù)量較少,且分布狀態(tài)較不均勻,表層出現(xiàn)缺損,潮線紊亂。與模型組相比,針刀組軟骨表面較光滑,且軟骨細(xì)胞分布較均勻,排列較規(guī)整,潮線較完整。與空白組比較,模型組軟骨組織Caspase-1、NLRP3、IL-1β mRNA表達(dá)顯著上調(diào)(P<0.05); 與模型組比較,針刀組軟骨組織Caspase-1、NLRP3、IL-1β mRNA表達(dá)顯著下調(diào)(P<0.05)。與空白組比較,模型組中兔軟骨組織Caspase-1、NLRP3、IL-1β mRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表達(dá)水平顯著升高(P<0.05)。針刀干預(yù)后兔軟骨細(xì)胞排列較模型組改善,軟骨細(xì)胞數(shù)量增多,Caspase-1、NLRP3、IL-1β mRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表達(dá)水平顯著下調(diào)(P<0.05)。結(jié)論 基于經(jīng)筋理論運(yùn)用針刀治療KOA,延緩軟骨病變,從而改善癥狀,其作用機(jī)制可能是通過干預(yù)軟骨細(xì)胞焦亡,從而降低關(guān)節(jié)內(nèi)炎癥介質(zhì)來實(shí)現(xiàn)的。
Abstract:
Objective To observe the effects of acupotomy on the regulation of the NLRP3/Caspase-1 pathway and chondrocyte pyroptosis in rabbit cartilage with knee osteoarthritis(KOA).Methods Twenty-four male New Zealand rabbits, all aged six months, were used.They were divided into three groups using the random number table method: blank group, model group, and acupotomy group.The modeling method was fixed by the modified Videman method for six weeks, and the blank group was not fixed.In the acupotomy group, the transversal tendon lesion was treated with needle knife, once a week, for a total of three times; the blank group and the model group were not treated.The morphological changes of cartilage were observed by HE staining, and the expression of cartilage Caspase-1, NLRP3, ASC and IL-1β in the cells were detected.Results The chondrocytes in the blank group were uniformly distributed in the cartilage matrix, with relatively smooth surface, clearer hierarchical structure and clear and complete tide lines.In the model group, the number of chondrocytes was smaller, and the distribution was uneven, with defects in the surface layer and disturbed tide lines.Compared with the model group, the cartilage surface of the needle knife group was smoother, and the chondrocytes were more evenly distributed and arranged, and the tide lines were more complete.Compared with the blank group, cartilage tissue Caspase-1, NLRP3, IL-1β mRNA expression was significantly up-regulated in the model group(P<0.05); compared with the model group, cartilage tissue Caspase-1, NLRP3, IL-1β mRNA expression was significantly down-regulated in the needle-knife group(P<0.05).Compared with the blank group, the expression levels of Caspase-1, NLRP3, IL-1β mRNA and Caspase-1, NLRP3, IL-1β, ASC protein in rabbit cartilage tissue were significantly increased in the model group(P<0.05).Chondrocyte alignment was improved in rabbits after acupotomy intervention compared with the model group, the number of chondrocytes was increased, and the expression levels of Caspase-1, NLRP3, IL-1β mRNA and Caspase-1, NLRP3, IL-1β, ASC protein were significantly downregulated(P<0.05).Conclusion Treating KOA with acupotomy based on the theory of meridian theory can delay cartilage lesions and improve symptoms.The mechanism of action may be reducing the intra-articular inflammatory mediators by intervening chondrocyte pyroptosis.

參考文獻(xiàn)/References:

[1] 徐浩, 肖漣波, 翟偉韜. 膝骨關(guān)節(jié)炎中西醫(yī)結(jié)合診療專家共識(shí)[J].世界中醫(yī)藥, 2023,18(7): 929 - 935.
[2] 中華醫(yī)學(xué)會(huì)骨科學(xué)分會(huì)關(guān)節(jié)外科學(xué)組.骨關(guān)節(jié)炎診治指南(2018版)[J].中華骨科雜志2018,38(12):705-715.
[3] 張良志, 劉洪, 修忠標(biāo).基于經(jīng)筋理論針刀治療膝骨性關(guān)節(jié)炎療效的Meta分析[J].中國民族民間醫(yī)藥, 2020, 29(8): 54-57.
[4] 方麗娜, 李上封, 袁紅麗,等.“膝六”“膝七”點(diǎn)針刀松解對早中期膝骨關(guān)節(jié)炎的療效研究[J].中國醫(yī)藥導(dǎo)報(bào), 2022, 19(18): 27-32.
[5] 修忠標(biāo), 張春霞, 劉晶.針刀治療膝骨性關(guān)節(jié)炎臨床觀察及機(jī)制探討[J].遼寧中醫(yī)藥大學(xué)學(xué)報(bào), 2018, 20(1):15 -18.
[6] Li G, Xiu L, Li X, et al.miR-155 inhibits chondrocyte pyroptosis in knee osteoarthritis by targeting SMAD2 and inhibiting the NLRP3/Caspase-1 pathway[J].J Orthop Surg Res,2022,17(1): 48.
[7] 林晴, 林煒, 付長龍,等. 細(xì)胞焦亡與關(guān)節(jié)炎的研究進(jìn)展[J].中醫(yī)正骨, 2018, 30(11):43-47.
[8] 劉晶, 林巧璇, 盧莉銘, 等.改良Videman法復(fù)制兔膝骨關(guān)節(jié)炎的實(shí)驗(yàn)研究[J].康復(fù)學(xué)報(bào), 2020, 30(3):212-219.
[9] 喻溢楠,唐成林,郭嘯,等.電針對膝骨關(guān)節(jié)炎大鼠膝關(guān)節(jié)滑膜組織細(xì)胞焦亡的影響[J].針刺研究,2022,47(6):471-478.
[10] 薛立功.中國經(jīng)筋學(xué)[M].北京: 中醫(yī)古籍出版社, 2009:681-728.
[11] 劉晶, 林巧璇, 盧莉銘,等.基于Wnt3a/β-catenin信號(hào)通路探討針刀對膝骨關(guān)節(jié)炎兔股直肌纖維化的影響[J].中華中醫(yī)藥雜志, 2022, 37(1):136-140.
[12] 文晨曦, 李敬揚(yáng).骨關(guān)節(jié)炎相關(guān)病理改變的發(fā)病機(jī)制及治療策略[J].生命的化學(xué), 2021, 41(9):1974-1980.
[13] DeFrate L E, Kim-Wang S Y, Englander Z A, et al.Osteoarthritis year in review 2018: mechanics[J].Osteoarthritis Cartilage,2019,27(3):392-400.
[14] Nakamachi E, Noma T, Nakahara K, et al. Multiphoton microscope measurement-based biphasic multiscale analyses of knee joint articular cartilage and chondrocyte by using visco-anisotropic hyperelastic finite element method and smoothed particle hydrodynamics method[J].Int J Numer Method Biomed Eng,2017,33(11).
[15] Bhutia S C, Sherpa M L, Dewan S K, et al. Correlation of cartilage metabolic markers & antioxidants with the severity of knee osteoarthritis[J].Indian J Med Res,2016,144(6):932-934.
[16] Shao H, Han G, Ling P, et al. Intra-articular injection of xanthan gum reduces pain and cartilage damage in a ratoste oarthritis model[J].Carbohydr Polym,2013,92(2):1850-1857.
[17] Yang Y H, Liu T H, Zhang L D, et al. Role of the PERK-eIF2α-CHOP signaling pathway in the effect of needle knife therapy on knee joint chondrocyte apoptosis[J].Evid Based Complement Alternat Med,2019,2019:7164916.
[18] 修忠標(biāo), 張春霞, 劉洪,等. 針刀治療膝骨性關(guān)節(jié)炎臨床觀察及機(jī)制探討[J]. 遼寧中醫(yī)藥大學(xué)學(xué)報(bào), 2018, 20(1):15-18.
[19] 修忠標(biāo), 劉洪, 張良志,等.針刀干預(yù)對膝骨關(guān)節(jié)炎兔原代軟骨細(xì)胞活性、凋亡及自噬的影響[J].中國醫(yī)藥導(dǎo)報(bào), 2022, 19(18):123-127.
[20] 劉晶,林巧璇,盧莉銘,等. 針刀“解結(jié)法”對膝骨關(guān)節(jié)炎兔軟骨形態(tài)學(xué)及影像學(xué)的影響[J].針刺研究, 2021, 46(2):129-135.
[21] Zhang X, Wang Q, Cao G, et al.Pyroptosis by NLRP3/Caspase-1/gasdermin-D pathway in synovial tissues of rheum atoid arthritis patients[J].J Cell Mol Med,2023,27(16):2448-2456.
[22] Liu J, Jia S, Yang Y, et al. Exercise induced meteorin-like protects chondrocytes against inflammation and pyroptosis in osteoarthritis by inhibiting PI3K/Akt/NF-κB and NLRP3/Caspase-1/GSDMD signaling[J].Biomed Pharmacother,2023,158:114118.

相似文獻(xiàn)/References:

[1]張良志,劉洪,楊金碩,等.針刀治療膝骨性關(guān)節(jié)炎的臨床療效及機(jī)制研究[J].福建醫(yī)藥雜志,2024,46(02):18.[doi:10.20148/j.fmj.2024.02.005]
　ZHANG Liangzhi,LIU Hong,YANG Jinshuo,et al.Clinical efficacy and mechanism of acupotomy in the treatment of knee osteoarthritis[J].FUJIAN MEDICAL JOURNAL,2024,46(05):18.[doi:10.20148/j.fmj.2024.02.005]
[2]鄭世雄,林煜,劉合亮,等.基于PI3K/Akt通路探討氟尿嘧啶對大鼠軟骨細(xì)胞凋亡的影響[J].福建醫(yī)藥雜志,2024,46(03):99.[doi:10.20148/j.fmj.2024.03.031]
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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金面上項(xiàng)目(2022J01354); 福建中醫(yī)藥大學(xué)中醫(yī)骨傷及運(yùn)動(dòng)康復(fù)教育部重點(diǎn)實(shí)驗(yàn)室開放課題(ZD2020-1-4)
1 福建省骨傷研究所; 2 中醫(yī)骨傷及運(yùn)動(dòng)康復(fù)教育部重點(diǎn)實(shí)驗(yàn)室; 3 福建中醫(yī)藥大學(xué); 4 通信作者,Email:[email protected]
更新日期/Last Update: 2023-10-15