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[1]鄭圓圓,黃浦,余真,等.孕期暴露DEHP對(duì)子代雌性大鼠下丘腦神經(jīng)內(nèi)分泌基因表達(dá)的影響[J].福建醫(yī)藥雜志,2023,45(04):93-97.
 ZHENG Yuanyuan,HUANG Pu,YU Zhen,et al.Effect of DEHP exposure during pregnancy on hypothalamic neuroendocrine gene expression in offspring female rats[J].FUJIAN MEDICAL JOURNAL,2023,45(04):93-97.
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孕期暴露DEHP對(duì)子代雌性大鼠下丘腦神經(jīng)內(nèi)分泌基因表達(dá)的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年04期
頁(yè)碼:
93-97
欄目:
基礎(chǔ)研究
出版日期:
2023-08-15

文章信息/Info

Title:
Effect of DEHP exposure during pregnancy on hypothalamic neuroendocrine gene expression in offspring female rats
文章編號(hào):
1002-2600(2023)04-0093-05
作者:
鄭圓圓黃浦1余真2溫俊平陳剛3
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院干部特診二科(福州 350001)
Author(s):
ZHENG Yuanyuan HUANG Pu YU Zhen WEN Junping CHEN Gang
The Second Department of Special Diagnosis for Cadres,Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University. Fuzhou, Fujian 350001, China
關(guān)鍵詞:
DEHP 孕期暴露 子代雌性大鼠 下丘腦神經(jīng)內(nèi)分泌基因
Keywords:
DEHP exposure during pregnancy offspring female mice hypothalamic neuroendocrine gene
分類(lèi)號(hào):
R714.2
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探究孕期DEHP暴露對(duì)后代下丘腦生殖和能量代謝相關(guān)神經(jīng)內(nèi)分泌基因的影響。方法 將16只受孕成功的SD大鼠隨機(jī)分為溶劑對(duì)照組(D0)和DEHP劑量500 mg/kg組(D500),從妊娠第1天開(kāi)始連續(xù)灌胃18 d,1次/d。F1代子鼠出生后第1天定為 PND0,記錄新生鼠體質(zhì)量、死胎、畸胎情況。F1代子鼠于28日齡時(shí)處死,采用熒光定量PCR技術(shù)檢測(cè)F1代雌鼠下丘腦生殖相關(guān)基因(Kiss1、GnRH)和能量代謝相關(guān)基因(Npy、Agrp、Pomc)的mRNA表達(dá)。同時(shí),采用下丘腦冷凍切片免疫熒光技術(shù)分析上述基因的蛋白表達(dá)水平。結(jié)果 孕期接觸DEHP可導(dǎo)致新生鼠體質(zhì)量下降,下丘腦GnRH mRNA表達(dá)增加,腹前室旁核(AVPV)區(qū)Kiss1基因及其編碼蛋白Kisspeptin表達(dá)增加。在下丘腦弓狀核中,Kiss1和Npy基因的mRNA和蛋白表達(dá)無(wú)明顯差異,Agrp基因mRNA和蛋白表達(dá)量顯著增加,Pomc基因mRNA和蛋白表達(dá)量顯著降低。結(jié)論 孕期DEHP暴露可影響子代雌性大鼠下丘腦生殖和能量代謝相關(guān)基因的轉(zhuǎn)錄和蛋白表達(dá)。
Abstract:
Objective To investigate the effect of DEHP exposure during pregnancy on hypothalamic reproduction and energy metabolism related neuroendocrine genes in offspring. Methods Sixteen pregnant SD rats were randomly divided into solvent control group(D0)and 500 mg/kg DEHP group(D500), which were given continuous gavage for 18 days from the first day of pregnancy, once a day. PND0 was set as the first day after birth of F1 generation offspring mice, and the body mass, litter size and sex ratio of newborn mice were recorded, as well as the existence of stillbirth and teratogenesis. The mice were sacrificed at 28 days of age, and the mRNA expression of reproductive genes(Kiss1,GnRH)and energy metabolism-related genes(Npy,Agrp,Pomc)in the hypothalamus of the offspring of female mice was detected by fluorescence quantitative PCR. Meanwhile, immunofluorescence technique was used to analyze the protein expression levels of the above genes. Results Exposure to DEHP during pregnancy resulted in body mass loss in neonatal mice. GnRH mRNA expression increased in hypothalamus, Kiss1 gene and its encoding protein kisspeptin expression increased in AVPV region. mRNA and protein expression of Kiss1 and Npy genes were not significantly different in the hypothalamic arcuate nucleus. The mRNA and protein expression of Agrp gene were significantly increased, while the mRNA and protein expression of Pomc gene were significantly decreased. Conclusion DEHP exposure during pregnancy can affect the transcription and protein expression of hypothalamic endocrine genes related to reproductive and energy metabolism in offspring female rats.

參考文獻(xiàn)/References:

[1] Lee D W,Kim M S,Lim Y H,et al.Prenatal and postnatal exposure to di-(2-ethylhexyl)phthalate and neurodevelopmentaloutcomes:a systematic review and meta-analysis[J].Environ Res,2018,167:558-566.
[2] Fan Y,Qin Y,Chen M,et al.Prenatal low-dose DEHP exposure induces metabolic adaptation and obesity:Role ofhepatic thiamine metabolism[J].J Hazard Mater,2020,385:121534.
[3] Gao N,Hu R,Huang Y,et al.Specific effects of prenatal DEHP exposure on neuroendocrine gene expression in thedeveloping hypothalamus of male rats[J].Arch Toxicol,2018,92(1):501-512.
[4] Rusyn I,Peters J M,Cunningham M L.Modes of action and species-specific effects of di-(2-ethylhexyl)phthalate in theliver[J].Crit Rev Toxicol,2006,6(5):459-479.
[5] Gupta R K,Singh J M,Leslie T C,et al.Di-(2-ethylhexyl)phthalate and mono-(2-ethylhexyl)phthalate inhibit growth and reduce estradiol levels of antral follicles in vitro[J].Toxicol Appl Pharmacol,2010,242(2):224-230.
[6] Koch H M,Bolt H M,Preuss R,et al.New metabolites of di(2-ethylhexyl)phthalate(DEHP)in human urine and serumafter single oral doses of deuterium-labelled DEHP[J].Arch Toxicol,2005,79(7):367-376.
[7] Mariana M,Feiteiro J,Cairrao E.Cardiovascular response of rat aorta to di-(2-ethylhexyl)phthalate(DEHP)exposure[J].Cardiovasc Toxicol,2018,18(4):356-364.
[8] Campbell Jr J L,Yoon M,Ward P,et al.Excretion of di-2-ethylhexyl phthalate(DEHP)metabolites in urine is related to body mass index because of higher energy intake in the overweight and obese[J].Environ Int,2018,113:91-99.
[9] Zhu F.Research progress of biotoxicity and control strategy of plasticizer DEHP[J].Shanxi Chemical Industry,2020,40(4):29-30,33.
[10] Ha M,Guan X,Wei L,et al.Di-(2-ethylhexyl)phthalate inhibits testosterone level through disturbed hypothalamic-pituitary-testis axis and ERK-mediated 5alpha-reductase 2[J].Sci Total Environ,2016:563-564,566-575.
[11] Goudarzi M,Haghi Karamallah M,Malayeri A,et al.Protective effect of alpha-lipoic acid on di-(2-ethylhexyl)phthalate-induced testicular toxicity in mice[J].Environ Sci Pollut Res Int,2020,27(12):13670-13678.
[12] Kalo D,Vitorino C A,Archilla C,et al.Mono(2-ethylhexyl)phthalate(MEHP)induces transcriptomicalterations in oocytes and their derived blastocysts[J].Toxicology,2019,421:59-73.
[13] Jacobs H M,Sant K E,Basnet A,et al.Embryonic exposure to mono(2-ethylhexyl)phthalate(MEHP)disruptspancreatic organogenesis in zebrafish(Danio rerio)[J].Chemosphere,2018,195:498-507.
[14] Qi W,Xu Q,Xu Y,et al.Effect of notch pathway on lipid accumulation induced by mono-2-ethylhexylphthalate on 3T3-L1 cells[J].Ecotoxicol Environ Saf,2021,208:111472.
[15] Hlisnikova H,Petrovicova I,Kolena B,et al.Effects and mechanisms of phthalates action on reproductive processesand reproductive health:a literature review[J].Int J Environ Res Public Health,2020,17(18):6811.
[16] Dong Ruina L S,Zhang M,et al.Effect of DEHP on the expression levels of Bax and Bcl-2 in mouse spermatogonialcells[J].Journal of Ecotoxicology,2017,12(5):270-277.
[17] Shao P,Wang Y,Zhang M,et al.The interference of DEHP in precocious puberty of females mediated by thehypothalamic IGF-1/PI3K/Akt/mTOR signaling pathway[J].Ecotoxicol Environ Saf,2019,181:362-369.
[18] de Roux N,Genin E,Carel J C,et al.Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derivedpeptide receptor GPR54[J].Proc Natl Acad Sci U S A,2003,100(19):10972-10976.
[19] Seminara S B,Messager S,Chatzidaki E E,et al.The GPR54 gene as a regulator of puberty[J].N Engl J Med,2003,349(17):1614-1627.
[20] Ruohonen S T,Poutanen M,Tena-Sempere M.Role of kisspeptins in the control of the hypothalamic-pituitary-ovarianaxis:old dogmas and new challenges[J].Fertil Steril,2020,114(3):465-474.
[21] Ibrahim R O,Omer S H,Fattah C N.The Correlation between hormonal disturbance in PCOS women and serum level of kisspeptin[J].Int J Endocrinol,2020:6237141.
[22] Padda J,Khalid K,Moosa A,et al.Role of Kisspeptin on hypothalamic-pituitary-gonadal pathology and its effect onreproduction[J].Cureus,2021,13(8):e17600.
[23] Lopez-Rodriguez D,Franssen D,Bakker J,et al.Cellular and molecular features of EDC exposure:consequences forthe GnRH network[J].Nat Rev Endocrinol,2021,17(2):83-96.
[24] Tao Ailin Z X,Feng X L.Research progress of Kisspeptin in the treatment of polycystic ovary syndrome[J].Advances in Modern Obstetrics and Gynecology,2022,31(9):714-717.
[25] Patterson M,Murphy K G,Thompson E L,et al.Administration of kisspeptin-54 into discrete regions of the hypothalamus potently increases plasma luteinising hormone and testosterone in male adult rats[J].J Neuroendocrinol,2006,18(5):349-354.
[26] Wassenaar P N H,Legler J.Systematic review and meta-analysis of early life exposure to di(2-ethylhexyl)phthalate and obesity related outcomes in rodents[J].Chemosphere,2017,188:174-181.
[27] Veiga-Lopez A,Pu Y,Gingrich J,et al.Obesogenic endocrine disrupting chemicals:identifying knowledge gaps[J].Trends Endocrinol Metab,2018,29(9):607-625.
[28] Sol C M,Santos S,Duijts L,et al.Fetal phthalates and bisphenols and childhood lipid and glucose metabolism.A population-based prospective cohort study[J].Environ Int,2020,144:106063.
[29] Sohn J W.Network of hypothalamic neurons that control appetite[J].BMB Rep,2015,48(4):229-233.

備注/Memo

備注/Memo:
基金項(xiàng)目:福建省衛(wèi)健委科技計(jì)劃青年科研課題(2021QNB002)
1 福建中醫(yī)藥大學(xué)(福州 350001); 2 福建省醫(yī)學(xué)科學(xué)研究院 福建省醫(yī)學(xué)測(cè)試重點(diǎn)實(shí)驗(yàn)室(福州 350001); 3 通信作者,Email: [email protected]
更新日期/Last Update: 2023-08-15