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[1]陳梅芳,林勇,張家欣,等.牽張離子通道通過(guò)miRNA-146a/NF-κB通路介導(dǎo)呼吸機(jī)相關(guān)性肺損傷的實(shí)驗(yàn)研究[J].福建醫(yī)藥雜志,2023,45(03):102-106.
　CHEN Meifang,LIN Yong,ZHANG Jiaxin,et al.Experimental study of stretch-activated channel contributing to ventilator-induced lung injury via miRNA-146a/NF-κB passway[J].FUJIAN MEDICAL JOURNAL,2023,45(03):102-106.
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牽張離子通道通過(guò)miRNA-146a/NF-κB通路介導(dǎo)呼吸機(jī)相關(guān)性肺損傷的實(shí)驗(yàn)研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年03期
頁(yè)碼:
102-106
欄目:
基礎(chǔ)研究
出版日期:
2023-06-15

文章信息/Info

Title:
Experimental study of stretch-activated channel contributing to ventilator-induced lung injury via miRNA-146a/NF-κB passway
文章編號(hào):
1002-2600(2023)03-0102-05
作者:
陳梅芳1; 林勇2; 張家欣; 呂曉釵; 王階波3
福建醫(yī)科大學(xué)附屬協(xié)和醫(yī)院心血管外科(福州 350001)
Author(s):
CHEN Meifang;  LIN Yong;  ZHANG Jiaxin;  LV Xiaochai;  WANG Jiebo
Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, China
關(guān)鍵詞:
牽張離子通道;  呼吸機(jī)誘導(dǎo)肺損傷;  炎癥
Keywords:
stretch-activated channels;  ventilator-induced lung injury;  inflammation
分類號(hào):
R614.2; R654.2
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探析呼吸機(jī)相關(guān)性肺損傷(VILI)的發(fā)生機(jī)制,為防治VILI提供新的理論依據(jù)。方法 以自主呼吸大鼠為對(duì)照組(S組),大潮氣量機(jī)械通氣大鼠為實(shí)驗(yàn)組(V組),以牽張離子通道(SAC)阻滯劑大鼠作為干預(yù)措施組(V+B組),每組各12只; 機(jī)械通氣后比較大鼠肺組織結(jié)構(gòu)和功能、miRNA-146a、NF-κB P65、炎癥因子(TNF-α、IL-6)表達(dá)有無(wú)不同。結(jié)果 機(jī)械通氣后大鼠肺組織結(jié)構(gòu)破壞明顯,氧合功能明顯減低,肺組織miRNA-146a表達(dá)明顯下調(diào),NF-κB P65表達(dá)上調(diào),肺部和全身炎癥因子濃度增加。相比V組大鼠,V+B組大鼠上述病變程度明顯減輕,肺組織miRNA-146a表達(dá)下調(diào)減緩,NF-κB P65表達(dá)上調(diào)程度減輕,肺部和全身炎癥因子濃度增加幅度減少。結(jié)論 SAC可能通過(guò)miRNA-146a/NF-κB途徑介導(dǎo)VILI發(fā)生,有望成為防治VILI的新靶點(diǎn)。
Abstract:
Objective To explore the mechanism of ventilator-induced lung injury(VILI)and to provide some evidences for the prophylaxis and the treatment of VILI. Methods The mice were divided into three groups: spontaneous inspiration(group S), high tidal volume ventilation(group V), and high tidal volume ventilation with stretch-activated channels(SAC)blocker(group V+B)with each group 12 mice. Lung morphology and function, miRNA-146a expression,NF-κB P65 expression and inflammatory factors(TNF-α,IL-6) concentration in the serum and lung were evaluated in the setting of mechanical ventilation. Results Serious lesion in the lung with an obviously impaired oxygenation were observed in the mice that received high tidal volume ventilation. The expression of miRNA-146a significantly reduced, and the NF-κB P65 increased on the contrary. Serum and pulmonary inflammatory level elevated after ventilation. Compared with group V, morphology abnormity and oxygenation dysfunction relieved in the mice in the group V+B. Higher miRNA-146a level, lower NF-κB P65 expression and lower local and systemic inflammatory levels were observed in the mice of group V+B. Conclusion SAC contributes to VILI through the miRNA-146a/NF-κB passway and is expected to be a novel target for the prophylaxis and the treatment of VILI.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金資助項(xiàng)目(2019J05082)
1 福建省心臟醫(yī)學(xué)中心; 2 通信作者; 3 麻醉科
更新日期/Last Update: 2023-06-15