80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少?

[1]黃永玲.Beagle犬牙周韌帶成纖維細胞和骨髓基質細胞體外成骨再生潛能的比較[J].福建醫(yī)藥雜志,2023,45(01):106-110.
 HUANG Yongling.Comparison of osteopenic potential of PDLFs and BMSCs in Beagle dog[J].FUJIAN MEDICAL JOURNAL,2023,45(01):106-110.
點擊復制

Beagle犬牙周韌帶成纖維細胞和骨髓基質細胞體外成骨再生潛能的比較()
分享到:

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年01期
頁碼:
106-110
欄目:
基礎研究
出版日期:
2023-02-15

文章信息/Info

Title:
Comparison of osteopenic potential of PDLFs and BMSCs in Beagle dog
文章編號:
1002-2600(2023)01-0106-05
作者:
黃永玲
福建省口腔疾病研究重點實驗室 福建省口腔生物材料工程技術研究中心 福建省高校口腔醫(yī)學重點實驗室 福建醫(yī)科大學口腔醫(yī)學院/附屬口腔醫(yī)院(福州 350002)
Author(s):
HUANG Yongling
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University & School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian 350002, Ch
關鍵詞:
骨髓基質細胞 牙周韌帶成纖維細胞 聚合酶鏈反應 堿性磷酸酶 骨保護素
Keywords:
bone marrow stromatolites cells periodontal ligament fibroblasts cells realtime PCR alkaline phosphatase osteoprotegerin
分類號:
R781
文獻標志碼:
A
摘要:
目的 比較Beagle犬牙周韌帶成纖維細胞(periodontal ligament fibroblasts cells,PDLFs)和骨髓基質細胞(bone marrow stromal cells,BMSCs)體外成骨潛能,為篩選合適的種子細胞促進牙周組織再生提供實驗依據。方法 體外通過茜素紅染色、Realtime-PCR比較兩種細胞堿性磷酸酶(alkaline phosphatase,ALP)及骨保護素(osteoprotegerin,OPG)的表達。結果 茜素紅染色顯示PDLFs成骨能力高于BMSCs; Realtime-PCR顯示,PDLFs的OPG表達量高于BMSCs,差別有統(tǒng)計學意義(P<0.05); PDLFs的ALP表達量雖低于BMSCs,但兩者之間差別無統(tǒng)計學意義(P>0.05)。結論 PDLFs體外成骨潛能不低于BMSCs。
Abstract:
Objective To compare the osteopenic potential between periodontal ligament fibroblasts cells(PDLFs) and bone marrow stromal cells(BMSCs)in Beagle dog, and provide foundation for selecting seed cells of periodontal tissue regeneration.Methods Alizarin red staining and realtime PCR were used to compare the expression of alkaline phosphatase(ALP)and osteoprotegerin(OPG)between PDLFs and BMSCs.Results The result of Alizarin red staining showed that the osteopenic potential of PDLFs was higher than that of BMSCs.Date of realtime PCR showed that the levels of OPG in PDLFs was higher than that in BMSCs(P<0.05); the levels of ALP in PDLFs was lower than that in BMSCs, but the result had no statistical significance(P>0.05).Conclusion The osteopenic potential of PDLFs was not lower than that of BMSCs.

參考文獻/References:

[1] Houshmand B, Behnia H, Khoshzaban A, et al.Osteoblastic differentiation of human stem cells derived from bone marrow and periodontal ligament under the effect of enamel matrix derivative and transforming growth factor-beta[J].Int J Oral Maxillofac Implants, 2013, 28(6): e440-e450.
[2] Tomokiyo A, Wada N, Maeda H.Periodontal ligament stem cells: Regenerative potency in periodontium[J].Stem Cells Dev, 2019, 28(15):974-985.
[3] 張夢涵, 何智琪, 鄧輝, 等.褪黑素對人牙周膜細胞成骨分化能力的影響[J].口腔醫(yī)學, 2021, 41(3):198-203.
[4] 李韋瑤, 鄧嘉強, 趙芳芳, 等.姜黃素對犬骨髓間充質干細胞遷移能力的影響[J].四川農業(yè)大學學報, 2022, 40(4): 24:536-542.
[5] 林敏魁, 黃永玲, 鐘泉, 等.比格犬骨髓基質細胞和牙周韌帶成纖維細胞體外再生組織能力的比較[J].福建醫(yī)科大學學報, 2011, 45(4):242-245.
[6] Tomokiyo A, Wada N, Maeda H.Periodontal ligament stem cells: regenerative potency in periodontium.[J].Stem Cells Dev, 2019, 28(15):974-985.
[7] Hu L, Liu Y, Wang S.Stem cell-based tooth and periodontal regeneration[J].Oral Dis, 2018,24(5):696-705.
[8] Nagata M, Iwasaki K, Akazawa K, et al.Conditioned medium from periodontal ligament stem cells enhances periodontal regeneration[J].Tissue Eng Part A, 2017, 23(9-10):367-377.
[9] Iwasaki K, Akazawa K, Nagata M, et al.The fate of transplanted periodontal ligament stem cells in surgically created periodontal defects in rats[J].Int J Mol Sci, 2019, 20(1):192.
[10] 金振宇,張志宏.犬骨髓基質細胞礦化不同階段對犬牙周膜干細胞增殖和分化的影響[J].口腔疾病防治, 2018, 26(11):699-705.
[11] Liu L, Guo S, Shi W, et al.Bone marrow mesenchymal stem cell-derived small extracellular vesicles promote periodontal regeneration[J].Tissue Eng Part A, 2021, 27(13-14):962-976.
[12] Tsumanuma Y, Iwata T, Washio K, et al.Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model[J].Biomaterials, 2011, 32(25):5819-5825.
[13] Zhou Q, Gu X, Dong J, et al.The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environmen[J].Artif Cells Nanomed Biotechnol, 2019, 47(1):3329-3337.
[14] 王松, 王啟松, 何夢嬌, 等.骨髓基質細胞膜片對骨質疏松大鼠牙周組織再生的影響[J].中華骨質疏松和骨礦鹽疾病雜志, 2019, 12(6):586-594.
[15] Inoue T, Osada H, Shiigai T, et al.An experimental study of osteogenesis by autografted dental pulp, periodontal ligament, and bone marrow in vivo[J].Bull Tokyo Dent Coll, 1993, 34(4):183-190.
[16] Grzesik W J, Kuzentsov S A, Uzawa K, et al.Normal human cementum-derived cells: isolation, clonal expansion, and in vitro and in vivo characterization[J].J Bone Miner Res, 1998, 13(10):1547-1554.
[17] 徐孟丹, 周詠, 皺多宏.拉布拉多犬骨髓基質干細胞與牙周干細胞裸鼠皮下成骨能力的比較研究[J].安徽醫(yī)科大學學報, 2016, 51(9):1244-1247.
[18] Yan X Z,Yang F, Jansen J A, et al.Cell-Based approaches in periodontal regeneration: a systematic review and meta-analysis of periodontal defect models in animal experimental work[J].Tissue Eng Part B Rev, 2015, 21(5):411-426.
[19] Tassi S A, Sergio N Z, Misawa M Y O, et al.Efficacy of stem cells on periodontal regeneration: Systematic review of pre-clinical studies[J].J Periodontal Res, 2017, 52(5):793-812.
[20] 王娉婷, 周盼, 鄭國瑩,等.吡咯烷二硫氨基甲酸干預脂多糖刺激下牙周膜干細胞RANKL和OPG的表達[J].中國組織工程研究, 2018, 22(21):3365-3370.
[21] Sengottuvelan A, Balasubramanian P, Will J, et al.Bioactivation of titanium dioxide scaffolds by ALP-functionalization[J].Bioact Mater, 2017, 2(2):108-115.
[22] 黃永玲, 駱凱,李艷芬,等.犬牙周膜細胞與骨髓基質細胞組織再生能力比較的實驗研究[J].福建醫(yī)科大學學報, 2016,50(6):365-369.

更新日期/Last Update: 2023-02-15