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[1]吳嘉旭,胡明超,王強(qiáng),等.NDC1基因在肝癌中的診斷及預(yù)后價值的生物信息學(xué)分析[J].福建醫(yī)藥雜志,2023,45(01):7-12.
　WU Jiaxu,HU Mingchao,WANG Qiang,et al.Bioinformatics analysis of the diagnostic and prognostic value of NDC1 gene in liver hepatocellular carcinoma[J].FUJIAN MEDICAL JOURNAL,2023,45(01):7-12.
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NDC1基因在肝癌中的診斷及預(yù)后價值的生物信息學(xué)分析()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期數(shù):
2023年01期
頁碼:
7-12
欄目:
臨床研究
出版日期:
2023-02-15

文章信息/Info

Title:
Bioinformatics analysis of the diagnostic and prognostic value of NDC1 gene in liver hepatocellular carcinoma
文章編號:
1002-2600(2023)01-0007-06
作者:
吳嘉旭; 胡明超; 王強(qiáng); 俞文淵; 許華1
南京醫(yī)科大學(xué)附屬江蘇盛澤醫(yī)院普外科 南京醫(yī)科大學(xué)康達(dá)學(xué)院盛澤臨床醫(yī)學(xué)院(蘇州 215228)
Author(s):
WU Jiaxu; HU Mingchao; WANG Qiang; YU Wenyuan; XU HUA
Shengze School of Clinical Medicine, Kangda College of Nanjing Medical University, Department of General Surgery, the Affiliated Jiangsu Shengze Hospital of Nanjing Medical University, Suzhou, Jiangsu 215228, China
關(guān)鍵詞:
肝癌;  NDC1基因;  預(yù)后;  生物信息學(xué)
Keywords:
liver hepatocellular carcinoma;  NDC1 gene;  prognosis;  bioinformatics
分類號:
R735.7
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 基于生物信息學(xué)的方法研究NDC1基因用于肝癌的診斷及預(yù)后評估方面的作用。方法 以TCGA數(shù)據(jù)庫和GTEx為發(fā)現(xiàn)數(shù)據(jù)庫,使用軟件下載所有肝癌患者以及健康人的level 3 HTSeq-TPM格式的RNA測序結(jié)果,繪制箱式圖及點圖用于統(tǒng)計NDC1基因在肝癌組織及正常組織中的差異化表達(dá)情況,并使用GEPIA作為驗證數(shù)據(jù)庫進(jìn)行佐證。繪制受試者曲線來評估NDC1基因用于診斷肝癌的效能。根據(jù)NDC1基因表達(dá)量的中位數(shù)將患者區(qū)分為NDC1高表達(dá)和低表達(dá)兩組,研究肝癌患者的臨床病理特征以及預(yù)后情況和NDC1基因表達(dá)的關(guān)系。繪制生存曲線,探究NDC1基因的高低表達(dá)與LIHC患者的生存情況的關(guān)系。結(jié)果 肝癌患者的NDC1的表達(dá)量為2.16(1.746～2.64),明顯大于正常組織中的1.449(1.263～1.644),差異有統(tǒng)計學(xué)意義(P<0.05); 同樣的,在55對配對組織中,NDC1基因在肝癌組織中的表達(dá)量3.47(3.028～3.749)也明顯高于對應(yīng)的癌旁正常組織中NDC1的表達(dá)量2.426(2.278～2.611),差異有統(tǒng)計學(xué)意義(P<0.05)。受試者曲線的結(jié)果顯示了NDC1用于診斷肝癌有著較高的敏感性[曲線下面積=0.843,95%置信區(qū)間為(0.809,0.877)]; 在針對NDC1基因表達(dá)和肝癌患者臨床病理特征以及預(yù)后的關(guān)系研究中發(fā)現(xiàn)NDC1高表達(dá)的肝癌患者相比NDC1低表達(dá)患者有著更晚的T分期(P<0.05)和病理分期(P<0.05); 此外,NDC1基因高表達(dá)的肝癌患者總體生存期也短于NDC1基因低表達(dá)的患者。結(jié)論 NDC1基因有潛力成為用于肝癌診斷和預(yù)后評估的新型生物學(xué)標(biāo)志物。
Abstract:
Objective To investigate the role of the NDC1 gene for the diagnosis and prognostic evaluation of liver hepatocellular carcinoma(LIHC)based on bioinformatics approach.Methods The TCGA and GTEx database were used as the discovery databases and RNA sequencing results in level 3 HTSeq-TPM format from all LIHC patients as well as healthy individuals were downloaded using R software,and box plots and dot plots were peformed for analyzing the differential expression of NDC1 gene in LIHC tissues and normal tissues,and GEPIA was used as a validation database for corroboration.Secondly,Receiver Operating Characteristic(ROC)curve was drawn to assess the efficacy of the NDC1 gene for the diagnosis of LIHC.Patients were divided into low NDC1 expression group and high NDC1 expression group based on the median NDC1 gene expression,and the relationship between clinicopathological characteristics and prognosis of patients with LIHC and NDC1 gene expression was investigated.The relationship between the high and low expression of NDC1 gene was explored by drawing the survival curve of LIHC patients.Results The expression of NDC1 in patients with LIHC was 2.16(1.746-2.64),which was significantly higher than that of 1.449(1.263-1.644)in normal tissues,and the difference was statistically significant(P<0.05); similarly,in 55 paired tissues,the expression of NDC1 gene in LIHC tissues was 3.47(3.028-3.749),which was also significantly higher than that in the adjacent normal tissues 2.426(2.278-2.611),with statistically significant difference(P<0.05).The results of ROC curves showed a high sensitivity of NDC1 for the diagnosis of LIHC[area under curve=0.843,95% confidence interval(0.809,0.877)]; In the study of the relationship between NDC1 gene expression and the clinicopathological characteristics and prognosis of LIHC patients,we found that the LIHC patients with high NDC1 expression had more advanced T-stage and pathological stage compared with those with low NDC1 expression(P<0.05); moreover,the overall survival of LIHC patients with high NDC1 gene expression was shorter than that of patients with low NDC1 gene expression.Conclusion The NDC1 gene has the potential to be a novel biological marker for the diagnosis and prognostic evaluation of LIHC.

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備注/Memo

備注/Memo:
基金項目:蘇州市醫(yī)學(xué)重點學(xué)科(SZXK202126); 南京醫(yī)科大學(xué)康達(dá)學(xué)院科研發(fā)展基金(KD2022KYJJZD015)
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更新日期/Last Update: 2023-02-15