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[1]傅明煒,王熱華,林鋒,等.SFRP1通過抑制Wnt/β-catenin通路促進心肌細胞增殖的分子機制[J].福建醫(yī)藥雜志,2022,44(06):117-120.
　Fu Mingwei,Wang Rehua,Lin Feng,et al.SFRP1 promotes cardiomyocyte proliferation via inhibiting the Wnt/β-catenin pathway[J].FUJIAN MEDICAL JOURNAL,2022,44(06):117-120.
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SFRP1通過抑制Wnt/β-catenin通路促進心肌細胞增殖的分子機制()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年06期
頁碼:
117-120
欄目:
基礎研究
出版日期:
2022-12-15

文章信息/Info

Title:
SFRP1 promotes cardiomyocyte proliferation via inhibiting the Wnt/β-catenin pathway
文章編號:
1002-2600（2022）06-0117-04
作者:
傅明煒; 王熱華; 林鋒; 郭延松
福建醫(yī)科大學省立臨床醫(yī)學院 福建省立醫(yī)院心內(nèi)科(福州 350001）
Author(s):
Fu Mingwei; Wang Rehua; Lin Feng; Guo Yansong
Department of Cardiology,Fujian Provincial Hospital,Provincial Clinical College of Fujian Medical University,Fuzhou,Fujian 350001,China
關鍵詞:
SFRP1; Wnt/β-catenin; 心肌細胞; 細胞增殖
Keywords:
SFRP1;  Wnt/β-catenin; cardiomyocyte; cell proliferation
分類號:
R541
文獻標志碼:
A
摘要:
目的 探討SFRP1是否通過抑制Wnt/β-catenin通路促進心肌細胞增殖。方法 選取c57BL/6雄性小鼠18只，出生后1 d、7 d及28 d的c57BL/6小鼠各6只分為3組，分離和提取心肌組織，檢測心肌組織中SFRP1的mRNA及蛋白表達水平。利用出生后1 d的乳鼠心臟培養(yǎng)小鼠原代心肌細胞，予SFRP1-shRNA慢病毒轉染細胞，免疫熒光檢測心肌細胞增殖標志物Ki67和PH3表達變化，Western blot檢測胞漿β-catenin蛋白表達變化。結果 SFRP1 mRNA和蛋白水平在小鼠出生后逐漸下降（P<0.05）。轉染SFRP1-shRNA慢病毒后，SFRP1蛋白表達水平均下降（P<0.05），其中shRNA-2干擾效果最好。與空載組相比，shRNA組細胞Ki67和PH3表達下降，同時胞漿β-catenin蛋白表達水平升高（P<0.05）。結論 SFRP1通過抑制Wnt/β-catenin信號通路促進心肌細胞增殖。
Abstract:
Objective To investigate whether SFRP1 promotes cardiomyocyte proliferation via inhibiting the Wnt/β-catenin pathway. Methods Six c57BL/6 mice were selected which were 1/7/28-day old, respectively. The myocardial tissue was isolated and extracted, and the mRNA and protein expression levels of SFRP1 in myocardial tissue were detected. Primary mouse cardiomyocytes were cultured and identified from 1-day-old mice. Cells were transfected with SFRP1-shRNA lentivirus or vector lentivirus, and the interference efficiency of shRNA was verified by WB, besides the shRNA with the best interference effect was selected for subsequent experiments. The expression of α -actinin and proliferation markers of cardiomyocytes (Ki67, PH3) were detected by immunofluorescence, and the expression of β-catenin protein in cytoplasm was detected by WB. Results The mRNA and protein expression of SFRP1 was highest in the myocardial tissue of mice which were 1-day old, and decreased gradually 7 and 28-day old. The primary cardiomyocytes of newborn mice were cultured successfully. The survival rate of cardiomyocytes was more than 95% by trypan blue staining, and α-actinin immunofluorescence staining showed that the purity of cardiomyocytes was more than 95%. Compared with the vector group, the expression levels of SFRP1 protein in SFRP1-shRNA lentivirus group were decreased (ALL P<0. 01), and the interference effect of shRNA-2 was the best. Compared to vector group, the expression of α-actinin, Ki67 and PH3 were decreased in shRNA group, and the expression of β-catenin protein in cytoplasm was increased (P<0. 01). Conclusion SFRP1 promotes cardiomyocyte proliferation via inhibiting the Wnt/β-catenin pathway.

參考文獻/References:

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備注/Memo

備注/Memo:
基金項目：福建省衛(wèi)生健康科研人才培養(yǎng)項目（2019-1-8）
更新日期/Last Update: 2022-12-15