80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少?

[1]王貞娜,姜雯雯,王真紅,等.子宮內(nèi)膜異位癥在位內(nèi)膜中自噬相關(guān)基因及ceRNA網(wǎng)絡(luò)的作用[J].福建醫(yī)藥雜志,2022,44(06):20-25.
 WANG Zhenna,JIANG Wenwen,WANG Zhenhong,et al.Effect of autophagy-related genes and ceRNA network in endometriosis eutopic endometrium[J].FUJIAN MEDICAL JOURNAL,2022,44(06):20-25.
點擊復(fù)制

子宮內(nèi)膜異位癥在位內(nèi)膜中自噬相關(guān)基因及ceRNA網(wǎng)絡(luò)的作用()
分享到:

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年06期
頁碼:
20-25
欄目:
臨床研究
出版日期:
2022-12-15

文章信息/Info

Title:
Effect of autophagy-related genes and ceRNA network in endometriosis eutopic endometrium
文章編號:
1002-2600(2022)06-0020-06
作者:
王貞娜姜雯雯王真紅張華樂
福建省婦幼保健院 福建醫(yī)科大學(xué)婦兒臨床醫(yī)學(xué)院(福州 350001)
Author(s):
WANG ZhennaJIANG WenwenWANG ZhenhongZHANG Huale
Department of Gynecology,F(xiàn)ujian Maternity and Child Health Hospital,College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,F(xiàn)ujian Medical University,F(xiàn)uzhou,F(xiàn)ujian 350001,China
關(guān)鍵詞:
子宮內(nèi)膜異位癥ceRNA網(wǎng)絡(luò)自噬
Keywords:
endometriosis ceRNAautophagy
分類號:
R711.71
文獻標志碼:
B
摘要:
目的 通過篩選子宮內(nèi)膜異位癥在位內(nèi)膜中的自噬相關(guān)基因,明晰競爭性內(nèi)源性RNA(ceRNA)網(wǎng)絡(luò)在子宮內(nèi)膜異位癥中的作用。方法 從GEO數(shù)據(jù)庫和Human Autophagy Database數(shù)據(jù)庫分別獲取子宮內(nèi)膜異位癥數(shù)據(jù)集和自噬相關(guān)基因列表,查找自噬相關(guān)基因,通過預(yù)測和相關(guān)性篩選miRNA和LncRNA并構(gòu)建ceRNA網(wǎng)絡(luò)。驗證篩選得到的關(guān)鍵基因在其他子宮內(nèi)膜異位癥在位內(nèi)膜數(shù)據(jù)集的表達及診斷效能。結(jié)果 子宮內(nèi)膜異位癥在位內(nèi)膜中有39個自噬相關(guān)基因存在表達差異,通過PPI查找到7個關(guān)鍵基因,功能富集在巨自噬、線粒體自噬和激活轉(zhuǎn)錄因子等,信號通路主要涉及E2F、FOXO、DNA損傷修復(fù)、FAS信號通路、PI3K/Akt/mTOR等。構(gòu)建11個ceRNA調(diào)控網(wǎng)絡(luò),包括4個mRNA(CASP3、HIF1A、CDKN1A、RPS6KB1)、7個miRNA(hsa-let-7e-5p、hsa-miR-124-3p、hsa-miR-199a-5p、hsa-miR-200c-3p、hsa-miR-24-3p、hsa-miR-30a-5p和hsa-miR-382-5p)和8個LncRNA(NORAD、TUG1、MALAT1、CYTOR、PVT1、EBLN3P、LINC02381和XIST)。結(jié)論 自噬相關(guān)基因參與的ceRNA網(wǎng)絡(luò)調(diào)控關(guān)系,可以為我們研究子宮內(nèi)膜異位癥診斷和治療靶點提供新的方向。
Abstract:
Objective The goal of this study was to identify the crucial autophagy-related genes in endometriosis and construct the competitive endogenous RNA (ceRNA) to further understand the pathogenesis of endometriosis. Methods Gene Expression Omnibus (GEO) database and Human Autophagy Database (HADb) were used to identify differentially expressed mRNAs, then the related miRNAs and lncRNAs were predicted through software to construct a ceRNAs network. At the same time, another endometriosis dataset was analyzed to confirm the levels of crucial mRNAs expression and ROC in the ceRNA network. Results The differential expression analysis revealed 39 differentially expressed autophagy-related genes. Seven hub genes were found by PPI, which had high diagnostic performance. The functions of 7 hub genes were enriched in macroautophagy, mitophagy and activating transcription factors etc. The signaling pathways mainly involved E2F, FOXO, DNA damage repair, FAS signaling pathway, PI3K/Akt/mTOR. 11ceRNA networks consisting of 4 mRNAs(CASP3, HIF1A, CDKN1Aand RPS6KB1), 7 miRNAs(hsa-let-7e-5p, hsa-miR-124-3p, hsa-miR-199a-5p, hsa-miR-200c-3p, hsa-miR-24-3p, hsa-miR-30a-5p and hsa-miR-382-5p), and 8 lncRNAs(NORAD, TUG1, MALAT1, CYTOR, PVT1, EBLN3P, LINC02381 and XIST) were constructed. Conclusion The ceRNA network regulatory relationship involving autophagy-related genes can provide new directions to study the diagnostic and therapeutic targets of endometriosis.

參考文獻/References:

[1] Chapron C, Marcellin L, Borghese B, et al. Rethinking mechanisms, diagnosis and management of endometriosis[J]. Nat Rev Endocrinol, 2019, 15(11):666-682.
[2] Yang H L, Mei J, Chang K K, et al. Autophagy in endometriosis[J]. Am J Transl Res, 2017, 9(11):4707-4725.
[3] Li M, Lu M S, Liu M L, et al. An Observation of the role of autophagy in patients with endometriosis of different stages during secretory phase and proliferative phase[J]. Curr Gene Ther, 2018, 18(5):286-295.
[4] Sabaie H, Amirinejad N, Asadi M R, et al. Molecular insight into the therapeutic potential of long non-coding rna-associated competing endogenous rna axes in alzheimers disease: a systematic scoping review[J]. Front Aging Neurosci, 2021, 13:742242.
[5] Barrett T, Wilhite S E, Ledoux P, et al. NCBI GEO: archive for functional genomics data sets-update[J]. Nucleic Acids Res, 2013, 41(Database issue):D991-D995.
[6] Wang N N, Dong J, Zhang L, et al. HAMdb: a database of human autophagy modulators with specific pathway and disease information[J]. J Cheminform, 2018, 10(1):34.
[7] von Mering C, Huynen M, Jaeggi D, et al. STRING: a database of predicted functional associations between proteins[J]. Nucleic Acids Res, 2003, 31(1):258-261.
[8] Li J H, Liu S, Zhou H, et al. starBase v2. 0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data[J]. Nucleic Acids Res, 2014, 42(Database issue):D92-D97.
[9] Li Y, Liu Y D, Chen S L, et al. Down-regulation of long non-coding RNA MALAT1 inhibits granulosa cell proliferation in endometriosis by up-regulating P21 via activation of the ERK/MAPK pathway[J]. Mol Hum Reprod, 2019, 25(1):17-29.
[10] Mai H, Xu H, Lin H, et al. LINC01541 Functions as a ceRNA to modulate the wnt/beta-catenin pathway by decoying miR-506-5p in endometriosis[J]. Reprod Sci, 2021, 28(3):665-674.
[11] Kaya C, Alay I, Guraslan H, et al. The role of serum caspase 3 levels in prediction of endometriosis severity[J]. Gynecologic and Obstetric Investigation, 2018, 83(6):576-585.
[12] Liu H, Zhang Z, Xiong W, et al. Hypoxia-inducible factor-1alpha promotes endometrial stromal cells migration and invasion by upregulating autophagy in endometriosis[J]. Reproduction, 2017, 153(6):809-820.
[13] Zhao S, Li G, Yang L, et al. Response-specific progestin resistance in a newly characterized Ishikawa human endometrial cancer subcell line resulting from long-term exposure to medroxyprogesterone acetate[J]. Oncol Lett, 2013, 5(1):139-144.
[14] Lu H, Li S, Wu Q. Retinoic acid regulates endometriotic stromal cell growth through upregulation of Beclin1[J]. Arch Gynecol Obstet, 2018, 297(1):93-99.
[15] Cheng Z. FoxO transcription factors in mitochondrial homeostasis[J]. Biochem J, 2022, 479(4):525-536.
[16] Liu Y, Qin X, Lu X, et al. Effects of inhibiting the PI3K/Akt/mTOR signaling pathway on the pain of sciatic endometriosis in a rat model[J]. Can J Physiol Pharmacol, 2019, 97(10):963-970.
[17] Choi J, Jo M, Lee E, et al. Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR[J]. Fertil Steril, 2015, 104(3):655-664.
[18] Da S L, Da B M, Da L C, et al. miR-532-3p: a possible altered miRNA in cumulus cells of infertile women with advanced endometriosis[J]. Reprod Biomed Online, 2021, 42(3):579-588.
[19] Hudson Q J, Proestling K, Perricos A, et al. The Role of Long Non-Coding RNAs in Endometriosis[J]. Int J Mol Sci, 2021, 22(21):11425.
[20] Li R, Sun R, Hicks G R, et al. Arabidopsis ribosomal pro-teins control vacuole trafficking and developmental programs through the regulation of lipid metabolism[J]. Proc Natl Acad Sci U S A, 2015, 112(1):89-98.
[21] Zafari N, Bahramy A, Zolbin M M, et al. microRNAs as novel diagnostic biomarkers in endometriosis patients: a systematic review and meta-analysis[J]. Expert Rev Mol Diagn, 2022, 22(4):479-495.
[22] Pokrovenko D A, Vozniuk V, Medvediev M V. MicroRNA let-7: A promising non-invasive biomarker for diagnosing and treating external genital endometriosis[J]. Turk J Obstet Gynecol, 2021, 18(4):291-297.
[23] Sahin C, Mamillapalli R, Yi K W, et al. microRNA Let-7b: A Novel treatment for endometriosis[J]. J Cell Mol Med, 2018, 22(11):5346-5353.
[24] Cho S, Mutlu L, Zhou Y, et al. Aromatase inhibitor regula-tes let-7 expression and let-7f-induced cell migration in endometrial cells from women with endometriosis[J]. Fertil Steril, 2016, 106(3):673-680.
[25] Wang H, Ni C, Xiao W, et al. Role of lncRNA FTX in invasion, metastasis, and epithelial-mesenchymal transition of endometrial stromal cells caused by endometriosis by regulating the PI3K/Akt signaling pathway[J]. Ann Transl Med, 2020, 8(22):1504.
[26] Wang X Q, Wu P L, Zeng C, et al. Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis[J]. Genes (Basel), 2022, 13(3):433.

相似文獻/References:

[1]張璇 王小紅 林妍 吳小玲.逐瘀湯對血瘀型子宮內(nèi)膜異位癥性不孕的療效觀察[J].福建醫(yī)藥雜志,2017,39(5):101.
[2]衣 歡 林丹玫 鄭祥欽 吳花敏 林 蓉 莊煒民 楊麗婷.鄰苯二甲酸酯暴露與子宮內(nèi)膜異位癥疼痛關(guān)系的研究[J].福建醫(yī)藥雜志,2019,41(03):5.
 YI Huan,LIN Danmei,ZHENG Xiangqin,et al.Study of association between exposure to phthalates and endometriosis-related pain[J].FUJIAN MEDICAL JOURNAL,2019,41(06):5.
[3]張燕,張正紅,林清強,等.雌激素在子宮內(nèi)膜異位癥中的作用及其調(diào)控[J].福建醫(yī)藥雜志,2021,43(03):119.
[4]王曉連.腹膜型子宮內(nèi)膜異位癥與不孕的關(guān)系[J].福建醫(yī)藥雜志,2021,43(03):121.

更新日期/Last Update: 2022-12-15