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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:

44

期數(shù):

2022年05期

頁碼:

127-130

欄目:

基礎(chǔ)研究

出版日期:

2022-10-15

文章信息/Info

Title:

An experimental study on the establishment of a mouse model of acute radiation bladder injury

作者:

陳慧軍; 張偉平; 翁劍飛¹; 陳麗玄; 鐘少藝; 林生明; 林瑋²

福建中醫(yī)藥大學(xué)附屬第二人民醫(yī)院（福州 350003）

Author(s):

CHEN Huijun; ZHANG Weiping; WENG Jianfei; CHEN Lixuan; ZHONG Shaoyi; LIN Shengming; LIN Wei

The Second Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350003, China

關(guān)鍵詞:

急性放射性膀胱損傷; 小鼠; X射線輻照儀; 動物模型

Keywords:

acute radiation bladder injury; mice; X-ray irradiator; animal model

分類號:

R694.6

文獻(xiàn)標(biāo)志碼:

B

摘要:

目的 探討小鼠急性放射性膀胱損傷（ARC）模型的建立方法。 方法 將24只雌性ICR小鼠分為4組各6只，采用X射線輻照儀單次盆腔局部照射，照射劑量分別為0、10、20和30 Gy。照射后觀察各組小鼠攝食、進(jìn)水量、體質(zhì)量和存活率，通過排尿行為模式研究檢測小鼠膀胱功能；1周后處死，光鏡下觀察膀胱組織病理學(xué)改變。 結(jié)果 0 Gy組小鼠進(jìn)食、飲水量和自主活動均正常，體質(zhì)量增加；1周后所有小鼠均存活，膀胱未見明顯新生血管，光鏡下見膀胱黏膜細(xì)胞層次清晰，黏膜下無水腫，無出血。10 Gy組小鼠進(jìn)食、飲水量下降不明顯，體質(zhì)量無明顯變化，排尿頻率未見明顯增加；1周后所有小鼠均存活，膀胱未見明顯新生血管，病理切片未見膀胱黏膜明顯缺失，黏膜下層見輕度水腫。20 Gy組照射后，小鼠攝食飲水量減少、體質(zhì)量下降，排尿頻率明顯增加；1周后僅有5只小鼠存活，膀胱可見少量新生血管，光鏡下觀察可見膀胱部分黏膜脫落，黏膜下層水腫明顯，部分區(qū)域見少量散在出血點(diǎn)。30 Gy組小鼠進(jìn)食、飲水量明顯下降，排尿頻率明顯增加；1周后僅有3只小鼠存活，膀胱見較多新生血管，病理切片可見膀胱黏膜壞死脫落，黏膜下層水腫明顯，并可見較多出血點(diǎn)。 結(jié)論 采用X射線輻照儀單次盆腔局部照射20 Gy能成功誘導(dǎo)小鼠ARC，該模型能較準(zhǔn)確地反映放射性膀胱損傷的臨床特征及病理特征，可用于后續(xù)相關(guān)的實(shí)驗(yàn)研究。

Abstract:

Objective To explore the establishment of the mouse model of acute radiation bladder injury. Methods Twenty-four female ICR mice were divided into 4 groups, 6 mice in each group, and were irradiated by X-ray irradiator in a single pelvic region, and the irradiation doses were 0, 10, 20 and 30 Gy, respectively. After irradiation, the food and water intake, body weight and survival rate of the mice in each group were observed.The bladder function of the mice was detected by the study of urination behavior patterns. The mice were sacrificed 1 week later, and the pathological changes of the bladder were observed under the microscope.Results The 0 Gy mice had normal food intake, water intake and voluntary activities, and their body weight increased.All mice survived after 1 week. No obvious neovascularization and bleeding was found in the bladder. In the 10 Gy group, the consumption of food and water did not decrease significantly, the body weight did not change significantly, and the frequency of urination did not increase significantly. After 1 week, all mice survived, no obvious neovascularization in the bladder, and no obvious absence of bladder mucosa in pathological sections. Mild edema was seen in the lower layer.After irradiation in the 20 Gy group, the food and water intake of the mice decreased, the body weight decreased, and the frequency of urination increased significantly. After 1 week, only 5 mice survived, and a small amount of new blood vessels could be seen in the bladder. Under the light microscope, part of the bladder mucosa was detached, and the submucosa was edema. Obviously, a small amount of scattered bleeding spots were seen in some areas. The amount of food and water in the 30 Gy group decreased significantly, and the frequency of urination increased significantly. After 1 week, only 3 mice survived. Many new blood vessels were seen in the bladder. Pathological sections showed that the bladder mucosa was necrotic and exfoliated, and the submucosal edema was obvious. Conclusion A single pelvic local irradiation of 20 Gy by X-ray irradiator can successfully induce acute radiation bladder injury in mice. This model can accurately reflect the clinical and pathological characteristics of radiation bladder injury, and can be used for subsequent related experimental studies.

備注/Memo

備注/Memo:

基金項(xiàng)目：福建省教育廳中青年教師教育科研項(xiàng)目（JAT190277）；福建省自然科學(xué)基金面上項(xiàng)目（2021J01879）
1通信作者，Email:[email protected]；2福建中醫(yī)藥大學(xué)動物實(shí)驗(yàn)中心（福州 350122）

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更新日期/Last Update: 2022-10-15