80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]丁婕,何宇敏,王曉萍.血漿中有機(jī)磷液相色譜-質(zhì)譜法測定方法的建立[J].福建醫(yī)藥雜志,2022,44(04):117-120.
　DING Jie,HE Yumin,WANG Xiaoping.Establishment of a liquid chromatography/mass spectrometry method for detecting the plasma concentration of organophosphorus[J].FUJIAN MEDICAL JOURNAL,2022,44(04):117-120.
點擊復(fù)制

血漿中有機(jī)磷液相色譜-質(zhì)譜法測定方法的建立()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年04期
頁碼:
117-120
欄目:
基礎(chǔ)研究
出版日期:
2022-08-15

文章信息/Info

Title:
Establishment of a liquid chromatography/mass spectrometry method for detecting the plasma concentration of organophosphorus
文章編號:
1002-2600(2022)04-0117-04
作者:
丁婕; 何宇敏; 王曉萍1
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院急診科 福建省急診醫(yī)學(xué)重點實驗室（福州 350001）
Author(s):
DING Jie;  HE Yumin;  WANG Xiaoping
Fujian Provincial Key Laboratory of Emergency Medicine;Department of Emergency Medicine, Fujian Provincial Hospital;Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, China
關(guān)鍵詞:
液相色譜-質(zhì)譜法; 有機(jī)磷; 定量檢測
Keywords:
liquid chromatography/mass spectrometry;  organophosphorus;  quantitative detection
分類號:
R.331
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 建立液相色譜-質(zhì)譜法（liquid chromatography/mass spectrometry，LC/MS）測定血漿中氧化樂果、敵敵畏、克百威、樂果濃度的方法。方法 使用純乙腈沉淀蛋白提取樣品后，利用LC/MS法對提取的樣品進(jìn)行檢測。對建立方法的線性、精密度、加標(biāo)回收率進(jìn)行評價。結(jié)果 氧化樂果在10~200 ng/mL、100~1 000 ng/mL的濃度范圍內(nèi)線性關(guān)系良好，最低定量限為10 ng/mL；敵敵畏、克百威、樂果在50~1 000 ng/mL、1 000~5 000 ng/mL的濃度范圍內(nèi)線性關(guān)系良好，最低定量限均為50 ng/mL；氧化樂果、敵敵畏、克百威、樂果的批內(nèi)精密度均在±10%范圍內(nèi)，批間精密度在±15%范圍內(nèi)氧化樂果的回收率在92.89%~98.35%，敵敵畏的回收率為94.36%~98.27%，克百威的回收率為92.74%~97.28%，樂果的回收率為90.36%~96.48%。結(jié)論 建立的LC/MS方法可用于血漿中有機(jī)磷的定性和定量檢測。
Abstract:
Objective To establish a liquid chromatography/mass spectrometry (LC/MS) method for detecting the plasma concentration of organophosphorus．Methods The samples were extracted by acetonitrile,and the extracted samples were detected by LC/MS．The linear of the method, precision and standard recovery rate were evaluated．Results The relationship between the sample concentration and the peak area of mass spectrum response was linear within 10~200 ng/mL,100~1 000 ng/mL in omethoate，and the minimum limit of quantitation was 10 ng/mL；The relationship between the sample concentration and the peak area of mass spectrum response was linear within 50~1 000 ng/mL,1 000~5 000 ng/mL in dichlorvos, carbofuran, dimethoate,and the minimum limit of quantitation was 50 ng/mL.The intraassay precision of omethoate, dichlorvos, carbofuran and dimethoate was within ±10%, and the inter-assay precision was within ±15%．The recovery rate of omethoate, dichlorvos, carbofuran, dimethoate were 92.89%~98.35%, 94.36%~98.27%, 92.74%~97.28%, 90.36%~96.48% respectively. Conclusion The LC/MS method we established can be used for the qualitative and quantitative detection of omethoate, dichlorvos, carbofuran and dimethoate in plasma.

參考文獻(xiàn)/References:

[1] 楊立山,盧中秋,田英平．急性有機(jī)磷農(nóng)藥中毒診治臨床專家共識(2016)[J]．中國急救醫(yī)學(xué)，2016,36(12):1057-1065.
[2] 張在其,梁仁,黃韜,等.全血、血漿、紅細(xì)胞膽堿酯酶活性在急性有機(jī)磷農(nóng)藥中毒病程中的變化及意義[J]．中華急診醫(yī)學(xué)雜志,2001,10(4):232-234.
[3] 楊立山,李文峰,馬曉,等．血液灌流對急性有機(jī)磷農(nóng)藥中毒血膽堿酯酶活力恢復(fù)的影響研究[J]．寧夏醫(yī)學(xué)雜志,2011,33(6)：509-511.
[4] 王新平,曹國輝.血藥濃度監(jiān)測在急性有機(jī)磷農(nóng)藥中毒救治中的作用[J]．山東醫(yī)藥,2010,50(9):96.
[5] 何宇敏，柯俊，章九云,等. 血漿中百草枯、敵草快液相色譜-質(zhì)譜法測定方法建立[J]．創(chuàng)傷與急診電子雜志，2020,8,(3): 111-117.
[6] 中華醫(yī)學(xué)會檢驗醫(yī)學(xué)分會. 液相色譜-質(zhì)譜臨床應(yīng)用建議[J]．中華檢驗醫(yī)學(xué)雜志, 2018, 40(10): 770-779.
[7] 李小娟，何穎霞，朱寶立, 等. 人血清中有機(jī)磷農(nóng)藥QuEChERS法提取的氣相色譜-質(zhì)譜聯(lián)用法測定[J]．中華勞動衛(wèi)生職業(yè)病雜志,2018,36(5):378-381.
[8] 董芳,李惠玲,馬婧.全血中有機(jī)磷農(nóng)藥和鼠藥測定的QuEChERS前處理法[J]．中華勞動衛(wèi)生職業(yè)病雜志,2016,34(10):782-785.
[9] 林抗美,馬麗娜,朱育菁,等. 樂果液相色譜與氣相色譜檢測方法比較[J]．中國農(nóng)學(xué)通報,2008,24(10):453-456.
[10] 林靜.質(zhì)譜聯(lián)用技術(shù)及其在臨床上的應(yīng)用[J]．醫(yī)學(xué)綜述,2010,16(4):628-631.
[11] 李鵬,柏澤新,夏侯秋錦,等. UPLC-MS/MS檢測人血中18忠有機(jī)磷及氨基酸酯類農(nóng)藥[J]．中國法醫(yī)學(xué)雜志,2017,32(1):51-54.
[12] 王賢親,林丹．LC-MS/MS測定人血漿5種有機(jī)磷的含量[J]．中國衛(wèi)生檢驗雜志,2008,18(12):2537-2539.
[13] Inoue S, Saito T, Mase H. Rapid simultaneous determination fore organophosphorus pesticides in human serum by LC-MS[J]．Journal of Pharmaceutical and Biomedical Analysis, 2007, 44: 258-264.
[14] 黃寶勇,肖志勇,歐陽喜輝. 不同貯存條件對蔬菜基質(zhì)中13種農(nóng)藥殘留穩(wěn)定性的影響[J]．農(nóng)藥科學(xué)與管理, 2013(2):29-34.
[15] 閔濤, 劉永濤, 徐子振,等. 常見有機(jī)磷農(nóng)藥穩(wěn)定性研究進(jìn)展[J]．毒理學(xué)雜志,2018,32(2): 164-167.

備注/Memo

備注/Memo:
基金項目：福建省自然科學(xué)基金項目（青年創(chuàng)新）（2020J05263）
1 通信作者
更新日期/Last Update: 2022-08-15