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[1]陳冬冬,陳嶸.microRNA-365對骨肉瘤大鼠巨噬細胞增殖和凋亡的影響[J].福建醫(yī)藥雜志,2022,44(02):116-119.
　CHEN Dongdong,CHEN Rong.Effect of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats[J].FUJIAN MEDICAL JOURNAL,2022,44(02):116-119.
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microRNA-365對骨肉瘤大鼠巨噬細胞增殖和凋亡的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年02期
頁碼:
116-119
欄目:
基礎研究
出版日期:
2022-04-15

文章信息/Info

Title:
Effect of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats
文章編號:
1002-2600(2022)02-0116-04
作者:
陳冬冬; 陳嶸1
廈門大學附屬福州第二醫(yī)院骨腫瘤科(福州 350007)
Author(s):
CHEN Dongdong;  CHEN Rong
Department of Orthopaedics Oncology, the Affiliated Fuzhou Municipal Second Hospital, Xiamen University, Fuzhou, Fujian 350007, China
關鍵詞:
miR-365;  骨肉瘤;  巨噬細胞
Keywords:
miR-365;  osteosarcoma;  macrophage
分類號:
R738.1
文獻標志碼:
B
摘要:
目的 探討microRNA-365(miR-365)對骨肉瘤大鼠巨噬細胞增殖和凋亡的影響。方法 構建骨肉瘤大鼠模型,采用右后肢外側皮下注射的方法將miR-365模擬物、miR-365陰性對照物、miR-365 抑制物、miR-365 抑制陰性對照物及生理鹽水注入到大鼠模型中,采用RT-PCR檢測骨肉瘤大鼠巨噬細胞miR-365、KRAS的mRNA表達,Western blot檢測骨肉瘤大鼠巨噬細胞周期相關蛋白cyclin D1 和 cyclin E的表達。結果 與miR-365陰性對照組、miR-365抑制組、miR-365抑制陰性對照組、空白組相比較,miR-365組的miR-365、KRAS的mRNA表達量及cyclin D1、cyclin E的蛋白表達升高,差異性有統(tǒng)計學意義(P<0.05); 與miR-365抑制陰性對照組、空白組相比較,miR-365抑制組的miR-365、KRAS的mRNA表達量及cyclin D1、cyclin E的蛋白表達降低,差異有統(tǒng)計學意義(P<0.05); miR-365陰性對照組、miR-365抑制陰性對照組、空白組之間miR-365、KRAS的mRNA表達量及cyclin D1、cyclin E的蛋白表達差異無統(tǒng)計學意義(P>0.05)。結論 miR-365能夠通過KRAS促進巨噬細胞的增殖,減輕生物體炎癥反應。
Abstract:
Objective To investigate the effects of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats.Methods The osteosarcoma rats model was established.miR-365 simulant,miR-365 negative control,miR-365 inhibitor,miR-365 inhibitor negative control and normal saline were injected into the rat model.The mRNA expression of miR-365 and KRAS in macrophages of osteosarcoma rats was detected by RT-PCR, and the expression of cyclin D1 and cyclin E were detected by Western blot.Results Compared with miR-365 negative control group, miR-365 inhibition group, miR-365 inhibition negative control group and blank group, the mRNA expression levels of miR-365 and KRAS and the protein expression levels of cyclin D1 and cyclin E in miR-365 group were increased, and the differences were statistically significant(P<0.05).Compared with miR-365 inhibition negative control group and blank group, the mRNA expression of miR-365and KRAS and the protein expression of cyclin D1 and cyclin E in miR-365 inhibition group weredecreased,and the differences were statistically significant(P<0.05).There were no significant differences in the mRNA expression of miR-365 and KRAS and the protein expression of cyclin D1 and cyclin E between miR-365 negative control group, miR-365 inhibition negative control group and blank group(P>0.05).Conclusion miR-365 can promote the proliferation of macrophages through KRAS and reduce the inflammatory reaction of organism.

參考文獻/References:

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備注/Memo

備注/Memo:
基金項目:2020年福州市衛(wèi)生健康科研創(chuàng)新團隊培育項目(2020-S-wt3)
1 通信作者,Email:[email protected]
更新日期/Last Update: 2022-04-15