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[1]劉海琴,沈冬祎,呂嘉婧,等.從Cyclin E1-CDK2-CKIs通路探討芪靈扶正清解方抑制肝癌細(xì)胞株Hep1-6增殖的作用機制[J].福建醫(yī)藥雜志,2022,44(01):111-114.
　LIU Haiqin,SHEN Dongyi,LV Jiajing,et al.Qiling Fuzheng Qingjie decoction inhibit the proliferation of hepatoma cell line Hep1-6 via Cyclin E1-CDK2-CKIs signaling pathway[J].FUJIAN MEDICAL JOURNAL,2022,44(01):111-114.
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從Cyclin E1-CDK2-CKIs通路探討芪靈扶正清解方抑制肝癌細(xì)胞株Hep1-6增殖的作用機制()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期數(shù):
2022年01期
頁碼:
111-114
欄目:
基礎(chǔ)研究
出版日期:
2022-02-15

文章信息/Info

Title:
Qiling Fuzheng Qingjie decoction inhibit the proliferation of hepatoma cell line Hep1-6 via Cyclin E1-CDK2-CKIs signaling pathway
文章編號:
1002-2600(2022)01-0111-04
作者:
劉海琴; 沈冬祎1; 呂嘉婧2; 羅秀2; 陳旭征; 林久茂; 章尤權(quán)3
福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合研究院福建省中西醫(yī)結(jié)合老年性疾病重點實驗室(福州350122)
Author(s):
LIU Haiqin;  SHEN Dongyi;  LV Jiajing;  LUO Xiu;  CHEN Xuzheng;  LIN J iumao;  ZHANG Youquan
Fujian Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine geriatric Diseases, Academy of Integrative Medicine， Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
關(guān)鍵詞:
芪靈扶正清解方;  Hep1-6細(xì)胞;  細(xì)胞周期;  CDK2;  CKIs
Keywords:
Qi Ling Fuzheng Qingjie decoction;  Hep1-6 cells;  cell cycle;  CDK2;  CKIs
分類號:
R73-3
文獻標(biāo)志碼:
A
摘要:
目的 從周期蛋白Cyclin E1-細(xì)胞周期依賴性蛋白激酶2(CDK2)-CDKs抑制因子CKIs (Cyclin E1-CDK2-CKIs)信號通路角度探討芪靈扶正清解方(QL)調(diào)控小鼠肝癌Hep1-6 細(xì)胞株增殖的機制。方法 體外培養(yǎng)Hep1-6細(xì)胞，分別給予不同劑量的QL醇提物(0、31.25、62.5、125、250、500、1000 ug/mL)干預(yù)Hep1-6細(xì)胞株24h、48h和72h。MTT法檢測細(xì)胞活力;流式細(xì)胞術(shù)檢測細(xì)胞周期各個時相的比例;Westernblot檢測CDK2、周期蛋白CyclinE1、視網(wǎng)膜母細(xì)胞瘤Rb、磷酸化Rb(p-Rb)以及CDKs抑制因子(CKIs) p57KIP2和p27KIP1的蛋白水平。結(jié)果 QL醇提物能顯著抑制Hepl-6細(xì)胞的生長活力，呈劑量依賴性; Hep1-6細(xì)胞周期被阻滯在G0/G1期，S期的DNA合成被抑制; QL醇提物下調(diào)了CDK2、Cyclin E1、p-Rb的蛋白水平，上調(diào)了p57KIP2和p27KIP1的蛋白水平。結(jié)論 QL方通過抑制CDK2與Cyclin E1的結(jié)合，同時正向調(diào)控CKIs抑制CyclinE1CDK2復(fù)合物的激酶活性，從兩方面抑制下游Rb的磷酸化水平，進而有效抑制肝癌Hepl-6細(xì)胞的增殖。
Abstract:
Objective To investigate the effects of Qiling Fuzheng Qingjie decoction (QL) on the proliferation of hepatoma cell line Hep1-6 via Cyclin E1-CDK2-CKIs signaling pathway. Methods Hep1-6 cells were cultured in vitro and treated with different doses of QL ethanol extract (0, 31.25, 62.5, 125, 250, 500, 1000 pg/mL) for 24 h, 48 h and 72 h, Cell viability was detected by MTT method; Flow cytometry was used to detect the ratio of each phase of the cell cycle; W estern blot was used to detect cycle-dependent protein kinases CDK2，Cyclin E1，retinoblastoma Rb, phosphorylated Rb (p-Rb) and CDKs inhibitor (CKIs) p57KIP2 and p27KIP1. Results QL ethanol extract could significantly inhibit the growth activity of Hep1-6 cells in a dose-dependent manner. Cell cycle was blocked in G0/G1 phase, and DNA synthesis in S phase was inhibited. The QL ethanol extract down regulated the protein levels of CDK2，Cyclin E1 and p-Rb，and up-regulated the protein levels of p57KIP2 and p27KIP1. Conclusion QL decoction could inhibit the proliferation of hepatoma Hep1-6 cells by inhibiting the binding of CDK2 and Cyclin E1 and positively regulating CKIs to inhibit the kinase activity of Cyclin E1-CDK2 complex, then inhibiting the phosphorylation of Rb.

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更新日期/Last Update: 2022-02-15