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[1]章尤權(quán),陳旭征,林瑤.松果菊苷對MPP+誘導(dǎo)的SH-SY5Y細(xì)胞氧化應(yīng)激損傷的保護(hù)作用[J].福建醫(yī)藥雜志,2021,43(04):126-128.
　Zhang Youquan,Chen Xuzheng,LIN Yao.Protective effect of Echinoside on oxidative stress injury induced by MPP+ in SH-SY5Y cells[J].FUJIAN MEDICAL JOURNAL,2021,43(04):126-128.
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松果菊苷對MPP+誘導(dǎo)的SH-SY5Y細(xì)胞氧化應(yīng)激損傷的保護(hù)作用 ()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期數(shù):
2021年04期
頁碼:
126-128
欄目:
基礎(chǔ)研究
出版日期:
2021-08-15

文章信息/Info

Title:
Protective effect of Echinoside on oxidative stress injury induced by MPP+ in SH-SY5Y cells
文章編號:
1002-2600(2021)04-0126-03
作者:
章尤權(quán); 陳旭征1; 林瑤2
福建中醫(yī)藥大學(xué)附屬第二人民醫(yī)院（福州 350001）
Author(s):
Zhang Youquan; Chen Xuzheng; LIN Yao
The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou，F(xiàn)ujian 350122, China
關(guān)鍵詞:
松果菊苷; 氧化應(yīng)激; 細(xì)胞凋亡; 帕金森病
Keywords:
echinoside;  oxidative stress;  apoptosis;  parkinson's disease
分類號:
R741
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討松果菊苷(Echinacoside，ECH)對1-甲基-4-苯基吡啶離子（MPP+）誘導(dǎo)的人神經(jīng)母細(xì)胞瘤細(xì)胞（SH-SY5Y)細(xì)胞氧化應(yīng)激損傷的影響及機(jī)制。 方法 選取對數(shù)生長期的SH-SY5Y細(xì)胞分為對照組(正常培養(yǎng)，不給予任何試劑干預(yù))、MPP+組(500 μmol/L MPP+處理細(xì)胞24 h)、ECH組(1、3、10、30、100 μmol/L ECH預(yù)處理細(xì)胞24 h后加500 μmol/L MPP+處理細(xì)胞24 h)。CCK8檢測細(xì)胞存活率，倒置相差顯微鏡觀察細(xì)胞形態(tài)，Hoechst33342熒光染色法觀察凋亡細(xì)胞，化學(xué)比色法檢測抗氧化酶SOD、CAT活力以及脂質(zhì)過氧化物丙二醛MDA含量。 結(jié)果 與對照組相比，MPP+組細(xì)胞存活率顯著下降；細(xì)胞生長狀態(tài)明顯變差；細(xì)胞內(nèi)抗氧化酶SOD 和CAT活力顯著下降，MDA 含量顯著升高；出現(xiàn)明顯細(xì)胞凋亡現(xiàn)象。松果菊苷預(yù)處理SH-SY5Y細(xì)胞24 h后，與MPP+組比較，細(xì)胞存活率顯著上升；細(xì)胞生長狀態(tài)明顯好轉(zhuǎn)；細(xì)胞內(nèi)SOD、CAT活力顯著上升，MDA含量明顯減少；細(xì)胞凋亡現(xiàn)象明顯緩解。 結(jié)論 松果菊苷對MPP+誘導(dǎo)氧化應(yīng)激損傷的 SH-SY5Y 細(xì)胞具有保護(hù)作用，可通過增強(qiáng)細(xì)胞內(nèi)源性抗氧化酶的活力、抑制脂質(zhì)過氧化物的生成來抑制細(xì)胞凋亡。
Abstract:
Objective To investigate the effects of Echinacoside (ECH) on oxidative stress injury induced by MPP+ in SH->SY5Y cells and its mechanism．Methods SH-SY5Y cells in logarithmic growth phase were divided into control group (normal culture, without any reagent intervention), MPP+ group (500 μmol/L MPP+ treated cells for 24 h), and ECH group (1, 3, 10, 30, 100 μmol/L ECH pretreated cells for 24 h and 500 μmol/L MPP+ treated cells for 24h)．The survival rate of cells was determined by CCK8, and cell morphology was observed by inverted phase contrast microscope．The activities of antioxidant enzymes SOD and CAT and the content of lipid peroxide malondialdehyde (MDA) were determined by chemical colorimetry．The apoptotic cells were observed by Hoechst33342 fluorescence staining．Results Compared with the control group, the survival rate of MPP+ group was significantly decreased; the cell growth status was significantly worse．The activities of SOD and CAT in cells were significantly decreased, while the content of MDA was significantly increased．There was obvious apoptotic phenomenon．The survival rate of SH-SY5Y cells pretreated with ECH for 24 h was significantly higher than that of MPP+ group．The cell growth state was improved obviously．The activities of SOD and CAT increased significantly, while the content of MDA decreased significantly．Apoptosis was significantly alleviated．Conclusion ECH could protect SH-SY5Y cells from oxidative stress injury induced by MPP+, and inhibit cell apoptosis by enhancing the activity of endogenous antioxidant enzymes and inhibiting the generation of lipid peroxides.

參考文獻(xiàn)/References:

[1]薛海燕,焦嬋媛,姚軍.肉蓯蓉總苷藥理作用的研究現(xiàn)狀[J].中國臨床藥理學(xué)雜志,2018,34(4)：486-488.
[2]Tian X Y,Li M X,Lin T,et al．A review on the structure and pharmacological activity of phenylethanoid glycosides[J]．Eur J Med Chem,2021,209:112563.
[3]Vera D,Eunsung J,Maral M．The role of oxidative stress in Parkinson's disease[J]．J Parkinsons Dis,2013,3(4)：461-491．
[4]Ni Y J,Deng J,Liu X,et al．Echinacoside reverses myocardial remodeling and improves heart function via regulating SIRT1/FOXO3a/MnSOD axis in HF rats induced by isoproterenol[J]．J Cell Mol Med,2021,25(1)：203-216.
[5]Tao Z,Zhang L H,Wu T,et al．Echinacoside ameliorates alcohol-induced oxidative stress and hepatic steatosis by affecting SREBP1c/FASN pathway via PPARα[J]．Food Chem Toxicol,2021,148:111956．

備注/Memo

備注/Memo:
基金項(xiàng)目：國家自然科學(xué)基金青年項(xiàng)目（81804156）；福建省自然科學(xué)基金項(xiàng)目（2018J01882）
1 福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合研究院 福建省中西醫(yī)結(jié)合老年性疾病重點(diǎn)實(shí)驗(yàn)室；
2 通信作者，福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合學(xué)院， Email：[email protected]
更新日期/Last Update: 2021-08-15