80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]張虹,張春,肖志勇.上皮膜蛋白1在鼻咽癌組織中的表達(dá)特征[J].福建醫(yī)藥雜志,2021,43(03):14-18.
　ZHANG Hong,ZHANG Chun,XIAO Zhiyong.Expressive features of epithelial membrane protein 1 in nasopharyngeal carcinoma[J].FUJIAN MEDICAL JOURNAL,2021,43(03):14-18.
點(diǎn)擊復(fù)制

上皮膜蛋白1在鼻咽癌組織中的表達(dá)特征()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期數(shù):
2021年03期
頁(yè)碼:
14-18
欄目:
臨床研究
出版日期:
2021-06-15

文章信息/Info

Title:
Expressive features of epithelial membrane protein 1 in nasopharyngeal carcinoma
文章編號(hào):
1002-2600(2021)03-0014-05
作者:
張虹; 張春1; 肖志勇
廈門大學(xué)附屬福州市第二醫(yī)院耳鼻咽喉頭頸外科(福州 350007)
Author(s):
ZHANG Hong;  ZHANG Chun;  XIAO Zhiyong
Department of Otorhinolaryngology Head and Neck Surgery,the Affiliated Fuzhou Municipal Se-cond Hospital,Xiamen University,Fuzhou,Fujian 350007,China
關(guān)鍵詞:
上皮膜蛋白1(EMP1);  鼻咽癌;  緊密連接
Keywords:
epithelial membrane protein 1(EMP1);  nasopharyngeal carcinoma;  tight junction
分類號(hào):
R739.63
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 探討上皮膜蛋白1(EMP1)在鼻咽癌組織與正常鼻黏膜組織中的表達(dá)差異及其與疾病發(fā)展的關(guān)系。方法 收集40例鼻咽癌患者的癌組織(實(shí)驗(yàn)組)與正常鼻黏膜組織(對(duì)照組)各40份標(biāo)本,RT-qPCR、免疫熒光染色和免疫印記技術(shù)檢測(cè)EMP1在兩種組織中的表達(dá)情況; 40例鼻咽癌標(biāo)本免疫印跡測(cè)定EMP1灰度值與其對(duì)應(yīng)的腫瘤分化程度、臨床分期進(jìn)行二元線性回歸分析。結(jié)果 與正常鼻黏膜上皮對(duì)比,鼻咽癌上皮細(xì)胞的EMP1表達(dá)明顯降低(P<0.05),腫瘤細(xì)胞EMP1的表達(dá)與腫瘤分化程度呈正相關(guān)(P<0.05),與臨床分期不存在線性關(guān)系(P>0.05)。結(jié)論 EMP1在鼻咽癌細(xì)胞和正常鼻黏膜細(xì)胞表達(dá)有差異,其值可能預(yù)示腫瘤分化程度。
Abstract:
Objective To investigate the expressive differences of epithelial membrane protein 1(EMP1)between nasopharyngeal carcinoma tissues and normal nasal mucosa tissues and its relationship with disease development.Methods The expression of EMP1 in 40 nasopharyngeal carcinoma tissues(experimental group)and normal nasal mucosa tissues(control group)were detected by RT-qPCR, immunofluorescence staining and immunoblotting.The gray value of EMP1 was determined by Western blot in 40 nasopharyngeal carcinoma specimens, and the binary linear regression analysis was performed with tumor differentiation degree and clinical stage.Results Compared with normal nasal mucosa epithelium cells, the expression of EMP1 in nasopharyngeal carcinoma epithelial cells was significantly decreased(P<0.05).The expression of EMP1 in tumor cells was positively correlated with the degree of tumor differentiation(P<0.05),but there was no significant linear relationship between EMP1 expression and clinical stage(P>0.05).Conclusion The expression of EMP1 in nasopharyngeal carcinoma cells is di-fferent from that in normal nasal mucosa cells, which may predict the degree of tumor differentiations.

參考文獻(xiàn)/References:

[1] 梁鋅,楊劍,高婷,等.中國(guó)鼻咽癌流行情況[J].中國(guó)腫瘤,2016,25(11):835-840.
[2] Petersson F.Nasopharyngeal carcinoma:a review[J]. Semin Diagn Pathol,2015,32(1):54-73.
[3] Huang C L,Guo R,Li J Y,et al.Nasopharyngeal carcinoma treated with intensity-modulated radiotherapy: clinical outcomes and patterns of failure among subsets of 8th AJCC stage IVa [J].Eur Radiol,2020,30(2):816-822.
[4] Busch M, Klein S, Groβe-kreul J, et al.p53, miR-34a and EMP1-newly identified targets of TFF3 signaling in Y79 retinoblastoma cells[J].Int J Mol Sci, 2019,20(17):E4129.
[5] Wang Y W, Cheng H L, Ding Y R, et al.EMP1, EMP 2, and EMP3 as novel therapeutic targets in human cancer[J]. Biochim Biophys Acta Rev Cancer, 2017,1868(1):199-211.
[6] 叢馨,張艷,俞光巖,等.上皮細(xì)胞間緊密連接的研究進(jìn)展[J].生理學(xué)報(bào),2016,68(4):492-504.
[7] Taylor V,Welcher A A,Program A E,et al.Epithelial membrane protein-1, peripheral myelin protein 22, and lens membrane protein 20 define a novel gene family[J]. Biol Chem, 1995, 270(48):28824-28833.
[8] Wulf P, Suter U.Embryonic expression of epithelial membrane protein 1 in early neurons[J]. Brain Res Dev Brain Res, 1999, 116(2):169-180.
[9] Sun G G,Zhao G,Lu Y F,et al.Association of EMP1 with gastric carcinoma invasion,sruvival and prognosis[J].Int J Oncol, 2014, 45(3): 1091-1098.
[10] Li H,Zhang X,Jiang X,et al.The expression and function of epithelial membrane protein 1 in laryngeal carcinoma[J].Int J Oncol,2017,50(1):141-148.
[11] 代曉華,張軍,鄒慧儒,等.上皮膜蛋白1真核表達(dá)載體的構(gòu)建及其對(duì)人舌鱗狀細(xì)胞癌細(xì)胞遷移和侵襲對(duì)影響[J].華西口腔醫(yī)學(xué)雜志,2016,34(4):398-403.
[12] Liu Y,Ding Y L,Nie Y T,et al.EMP1 promotes the proliferation and invasion of ovarian cancer cells through activating the MAPK pathway[J].Onco Targets and Therapy,2020,13:2047-2055.
[13] Lin B,Zhang T,Ye X,et al.High expression of EMP1 predicts a poor prognosis and correlates with immune infiltrates in bladder urothelial carcinoma[J].ONcol Lett,2020,20(3):2840-2854.
[14] Echevarria M I,Awasthi S,Cheng C H,et al.African American specific gene panel predictive of poor prostate cancer outcome[J].J Urol,2019,202(2):247-255.
[15] Bangsow T,Baumann E,Bangsow C,et al.The epithelial membrane protein 1 is a novel tight junction protein of the blood-brain barrier[J].J Cereb Flow Metab,2008,28(6):1249-1260.
[16] Tzanakakis G,Kavasi R M,Voudouri K,et al.Role of the extracellular matrix in cancer-associated epithelial to mesenchymal transition phenomenon[J].Developmental Dynamics,2018,247(3):368-381.
[17] Durgan J,Tao G B,Walters M S,et al.SOS1 and Ras regulate epithelial tight junction formation in the human airway through EMP1[J].EMBO Rep,2015,16(1):87-96.

備注/Memo

備注/Memo:
1 福建醫(yī)科大學(xué)附屬福建省腫瘤醫(yī)院頭頸放療科
更新日期/Last Update: 2021-06-15