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[1]柳明忠 曾榮東 施建輝 謝俊杰.骨形態(tài)發(fā)生蛋白-4在創(chuàng)傷性骨化性肌炎大鼠模型的表達(dá)及意義[J].福建醫(yī)藥雜志,2020,42(06):131-134.
　LIU Mingzhong,ZENG Rongdong,SHI Jianhui,et al.Expression and significance of bone morphogenetic protein-4 in rat model of traumatic ossifying myositis[J].FUJIAN MEDICAL JOURNAL,2020,42(06):131-134.
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骨形態(tài)發(fā)生蛋白-4在創(chuàng)傷性骨化性肌炎大鼠模型的表達(dá)及意義()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年06期
頁(yè)碼:
131-134
欄目:
基礎(chǔ)研究
出版日期:
2020-12-15

文章信息/Info

Title:
Expression and significance of bone morphogenetic protein-4 in rat model of traumatic ossifying myositis
文章編號(hào):
1002-2600(2020)06-0131-04
作者:
柳明忠 曾榮東 施建輝 謝俊杰
福建省泉州市第一醫(yī)院骨科(泉州 362000)
Author(s):
LIU Mingzhong; ZENG Rongdong; SHI Jianhui; XIE Junjie.
Department of orthopedics, Quanzhou First Hospital Affiliated Fujian Medical University, Quanzhou,Fujian362000,China
關(guān)鍵詞:
骨形態(tài)發(fā)生蛋白-4;  骨化性肌炎;  塞來(lái)昔布
Keywords:
BMP-4;  traumatic ossifying myositis;  celecoxib
分類號(hào):
R641
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 探討骨形態(tài)發(fā)生蛋白-4(BMP-4)在大鼠創(chuàng)傷性骨化性肌炎(TMO)模型的表達(dá)及意義。方法 選取60只4～6周齡SD大鼠,隨機(jī)分為對(duì)照組、模型組和藥物組,模型組和藥物組均建立大鼠右側(cè)跟腱創(chuàng)傷性異位骨化模型,藥物組大鼠予塞來(lái)昔布10 mg/(kg·d)灌胃,模型組、對(duì)照組分別灌胃2 mL生理鹽水,于第5、10周各麻醉30只實(shí)驗(yàn)動(dòng)物,拍片觀察異位骨化形成情況,定量分析BMP-4與IL-2基因表達(dá),以Western blot檢測(cè)BMP-4蛋白的表達(dá)。結(jié)果 模型組與藥物組跟腱均有異位骨的形成,藥物組跟腱異位骨化形成速率低于模型組,發(fā)生成骨時(shí)間較晚,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。術(shù)后實(shí)時(shí)定量PCR檢測(cè)數(shù)據(jù)表明,模型組大鼠右跟腱組織中BMP-4基因表達(dá)明顯高于左跟腱組織,藥物組大鼠右跟腱組織中BMP-4基因表達(dá)顯著降低。蛋白質(zhì)免疫印跡檢查組織中BMP-4蛋白的表達(dá)發(fā)現(xiàn),藥物組大鼠骨化組織部位BMP-4蛋白明顯低于模型組。免疫組化結(jié)果表明,模型組大鼠組織中BMP-4蛋白表達(dá)的陽(yáng)性率明顯高于同只大鼠左腿對(duì)照組織; 藥物組大鼠跟腱組織中BMP-4表達(dá)的陽(yáng)性率較模型組降低,并藥物組BMP-4表達(dá)陽(yáng)性率隨著用藥時(shí)間的延長(zhǎng)而降低,跟腱組織中BMP-4表達(dá)的陽(yáng)性率與模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 BMP-4在創(chuàng)傷性骨化性肌炎大鼠模型的表達(dá)與骨化程度呈正相關(guān),塞來(lái)昔布可有效地減少或減輕大鼠跟腱術(shù)后炎癥反應(yīng),抑制BMP-4表達(dá),從而減緩創(chuàng)傷性骨化性肌炎的發(fā)生。
Abstract:
Objective To investigate the expression and significance of bone morphogenetic protein-4 in rat model of traumatic ossifying myositis.Methods Sixty SD rats aged 4-6 weeks were randomly divided into control group, model group and drug feeding group.Both model group and drug feeding group established traumatic heterotopic ossification model in the right Achilles tendon of rats.Rats in drug feeding group were given celecoxib 10 mg/(kg·d)gavage, and rats in model group and control group were given 2 mL normal saline respectively.Experimental animals were killed at 5 and 10 weeks respectively.X-ray film was used to examine the formation of new bone.The expression of bmp-4 and IL-2 genes were quantitatively analyzed by taking samples, and the expression of bmp-4 protein was detected by Western blot.Results The ossification degree of the model group was lighter than that of the model group, and the achilles tendon of the model group and the drug group increased gradually with the experimental cycle, and the formation of heterotopic bone was observed in both groups.However, the overall formation rate of the model group was faster than that of the drug group, and the osteogenesis time was earlier than that of the drug group, with a significant difference(P<0.05).QPCR detection showed that the expression of bmp-4 gene in the right achilles tendon tissue of the model group was significantly higher than that in the left achilles tendon tissue of the model group five weeks after surgery, while the expression of bmp-4 gene in the right achilles tendon tissue of the cecb group was significantly lower than that in the model group.Protein expression of bmp-4 in tissue by western blot showed that bmp-4 protein in ossified tissue of rats in the drug feeding group was significantly lower than that in the model group.Immunohistochemical results showed that the positive rate of bmp-4 protein expression in rat tissues of the model group was significantly higher than that in the left leg of the same rat.The positive rate of bmp-4 expression in the achilles tendon tissue of rats in the feeding group was significantly decreased, and with the extension of administration time, the positive rate of bmp-4 expression in the achilles tendon tissue was more and more significantly different from that in the model group.Conclusion Celecoxib can effectively reduce or alleviate the occurrence of traumatic ossifying myositis after Achilles tendon surgery in rats, which may be related to the inhibition of bmp-4 expression by celecoxib.

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備注/Memo

備注/Memo:
基金項(xiàng)目:泉州市科技計(jì)劃項(xiàng)目(2018Z081)
更新日期/Last Update: 1900-01-01