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[1]郭 丹 黃 慧 倪 峰 劉 煒 王玉婷 張?jiān)路?有機(jī)磷農(nóng)藥、高糖高脂飲食對(duì)二乙基亞硝胺誘發(fā)的大鼠肝癌模型肝組織損傷的實(shí)驗(yàn)研究[J].福建醫(yī)藥雜志,2020,42(06):128-131.
　GUO Dan,HUANG Hui,NI Feng,et al.Effects of organophosphorus pesticides, high-sugar and high-fat diet on liver tissue pathology in rat liver cancer model induced by diethylnitrosamine[J].FUJIAN MEDICAL JOURNAL,2020,42(06):128-131.
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有機(jī)磷農(nóng)藥、高糖高脂飲食對(duì)二乙基亞硝胺誘發(fā)的大鼠肝癌模型肝組織損傷的實(shí)驗(yàn)研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年06期
頁(yè)碼:
128-131
欄目:
基礎(chǔ)研究
出版日期:
2020-12-15

文章信息/Info

Title:
Effects of organophosphorus pesticides, high-sugar and high-fat diet on liver tissue pathology in rat liver cancer model induced by diethylnitrosamine
文章編號(hào):
1002-2600(2020)06-0128-04
作者:
郭 丹 黃 慧 倪 峰1 劉 煒 王玉婷 張?jiān)路?/a>
福建衛(wèi)生職業(yè)技術(shù)學(xué)院(福州 350101)
Author(s):
GUO Dan;  HUANG Hui;  NI Feng;  LIU Wei;  WANG Yuting;  ZHANG Yuefen.
Fujian Health College, Fuzhou, Fujian350101,China
關(guān)鍵詞:
農(nóng)藥;  高糖高脂;  二乙基亞硝胺;  肝癌模型;  SD大鼠
Keywords:
pesticide;  high-sugar and high-fat diet;  diethylnitrosamine(DEN);  liver cancer model;  SD rat
分類號(hào):
R735.7
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 研究低劑量三唑磷暴露、高糖高脂飲食對(duì)二乙基亞硝胺(DEN)誘發(fā)的大鼠肝癌模型肝組織病理的影響。方法 將正常6周齡雄性SD大鼠70只隨機(jī)分為4組,分別為對(duì)照組(10只)、DEN組(20只)、農(nóng)藥組(20只)、雙高組(20只)。除對(duì)照組外,其余3組分別進(jìn)行間歇腹腔注射DEN、腹腔注射DEN+有機(jī)磷農(nóng)藥飲水、腹腔注射DEN+高脂高糖飼料的處理,觀察大鼠的飲食、毛色、精神及活動(dòng)情況,每周測(cè)量大鼠體質(zhì)量,每隔4周解剖部分大鼠觀察肝臟形態(tài),造模16周時(shí)解剖大鼠觀察肝臟病理學(xué)形態(tài)。結(jié)果 對(duì)照組大鼠飲食活動(dòng)正常,毛色自然。實(shí)驗(yàn)組隨著DEN注射次數(shù)的增加,均出現(xiàn)不同程度的消瘦、脫毛、毛發(fā)枯槁、活動(dòng)減少等現(xiàn)象。造模后,各實(shí)驗(yàn)組大鼠平均體質(zhì)量均低于對(duì)照組。實(shí)驗(yàn)第16周時(shí),DEN組、農(nóng)藥組及雙高組的肝臟形態(tài)皆出現(xiàn)明顯的病理改變,各組的病變嚴(yán)重程度為:雙高組>農(nóng)藥組>DEN組。 結(jié)論 農(nóng)藥污染、高糖高脂飲食可促進(jìn)DEN誘導(dǎo)肝臟由肝炎-肝硬化的發(fā)展過(guò)程; 施加低劑量三唑磷、高糖高脂飲食因素誘導(dǎo)的肝癌發(fā)展過(guò)程在組織形態(tài)學(xué)上更接近臨床真實(shí)病例,這為改進(jìn)DEN肝癌大鼠模型提供有力的實(shí)驗(yàn)依據(jù)。
Abstract:
Objective To study the effects of low-dose triazophos exposure, high-sugar and high-fat diet on the pathological changes of liver tissue in rat liver cancer models induced by diethylnitrosamine(DEN).Methods A total of 70 normal male SD rats,6-week-old,were randomly divided into 4 groups, namely the control group(n=10), the DEN group(n=20), the pesticide group(n=20), and the Shuang Gao group(n=20).Except for the control group, the other 3 groups were treated with intermittent hypodermic injection of DEN, hypodermic injection of DEN plus drinking water added with triazophos, and hypodermic injection of DEN plus high-fat and high-sugar feed.The diet, hair color, and activity of rats were observed weekly.The body weight of the rats was measured every week.Some rats were dissected to observe liver morphology every 4 weeks.HE staining was used to obtain the liver pathology at week 16. Results The rats in the control group had normal eating activities and natural hair color.With increasing number of DEN injections in the experimental groups, there were various degrees of weight loss, hair loss, atrophy, and decreased activity.After modeling, the average weight of rats in each experimental group was lower than that of the control group.At the 16th week of the experiment, the liver morphology of the DEN group, the pesticide group and the Shuang Gao group showed obvious pathological changes.The severity of liver fibrosis in each group was: Shuang Gao grouP>pesticide grouP>DEN group.Conclusion Low-dose triazophos exposure, high-sugar and high-fat diet can promote the development of DEN-induced liver cancer.Histological morphology of the liver cancer model induced by low-dose triazophos, high-sugar and high-fat diet is closer to that of clinical liver cancer cases, which provides powerful experimental basis for improving DEN liver cancer rat model.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省科技計(jì)劃引導(dǎo)性項(xiàng)目(2019Y0051)
更新日期/Last Update: 1900-01-01