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[1]陳冬冬 鄭 竑.聚合物納米纖維-多孔鎂合金雙層支架的構(gòu)建及對(duì)成骨細(xì)胞活性的影響[J].福建醫(yī)藥雜志,2020,42(03):20-23.
　CHEN Dongdong,ZHEN Hong..Construction of polymer nanofiber porous magnesium alloy bilayer scaffold and its effect on osteoblast activity[J].FUJIAN MEDICAL JOURNAL,2020,42(03):20-23.
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聚合物納米纖維-多孔鎂合金雙層支架的構(gòu)建及對(duì)成骨細(xì)胞活性的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年03期
頁(yè)碼:
20-23
欄目:
臨床研究
出版日期:
2020-07-04

文章信息/Info

Title:
Construction of polymer nanofiber porous magnesium alloy bilayer scaffold and its effect on osteoblast activity
文章編號(hào):
1002-2600(2020)03-0020-04
作者:
陳冬冬 鄭 竑1
福建省福州市第二醫(yī)院骨科(福州 350007)
Author(s):
CHEN Dongdong;  ZHEN Hong.
Department of Orthopaedics, Fuzhou Municipal Second Hospital,Fuzhou, Fujian 350007, China
關(guān)鍵詞:
PLA聚合物納米纖維-多孔鎂合金雙層支架;  PLA聚合物納米纖維-TCP復(fù)合支架;  MC3T3-E1Q前成骨細(xì)胞;  生物活性
Keywords:
PLA polymer nanofiber porous magnesium alloy bilayer scaffold;  PLA polymer nanofiber-TCP composite scaffold;  MC3T3-E1Q osteoblasts;  bioactivity
分類號(hào):
R608
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 制備一種聚合物納米纖維-多孔鎂合金雙層支架,并考察其體外降解性能及對(duì)MC3T3-E1Q前成骨細(xì)胞生物活性的影響。方法 使用熱致相分離法(TIPS)在多孔鎂合金支架外層覆蓋一層聚合物納米纖維,將PLA聚合物納米纖維-多孔鎂合金雙層支架和PLA聚合物納米纖維-TCP復(fù)合支架進(jìn)行對(duì)比,采用電子天平稱重、熒光顯微細(xì)胞計(jì)數(shù)、MTT法、鈣黃綠素染色分別測(cè)定兩種支架的失重率、對(duì)MC3T3-E1Q前成骨細(xì)胞黏附、增殖和分化的影響。結(jié)果 PLA聚合物納米纖維-多孔鎂合金雙層支架的失重率、黏附的MC3T3-E1Q前成骨細(xì)胞數(shù)量、MC3T3-E1Q前成骨細(xì)胞增殖數(shù)量、鈣化結(jié)節(jié)數(shù)量及面積較PLA聚合物納米纖維-TCP復(fù)合支架均有顯著優(yōu)勢(shì),差異均具有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 成功制備了一種具有良好生物學(xué)功能及快降解速率的PLA聚合物納米纖維-多孔鎂合金雙層支架,具有廣泛的應(yīng)用前景。
Abstract:
Objective To construct a polymer nanofiber porous magnesium alloy bilayer scaffold and investigate its de-gradation in vitro and its effect on the activity of MC3T3-E1Q osteoblasts.Methods A thermally induced phase separation(TIPS)method was used to coat a layer of polymer nanofibers on the outer surface of the porous magnesium alloy stent.The PLA polymer nanofiber porous magnesium alloy bilayer scaffold and PLA polymer nanofiber-TCP composite scaffold were compared.The weight loss rate,the adhesion, proliferation and differentiation of two kinds of scaffolds were measured by electronic balance, fluorescence microscopic cell count, MTT method and calcein staining.Results The weight loss rate, the number of MC3T3-E1Q osteoblasts adhered to the PLA polymer nanofiber porous magnesium alloy bilayer scaffold, the number of MC3T3-E1Q osteoblasts proliferation, the number and area of the calcified nodules in the MC3T3-E1Q osteoblasts had significant advantages, and the differences were statistically significant(P<0.05).Conclusion PLA polymer nanofiber porous magnesium alloy bilayer scaffold with good biological function and fast degradation rate has been successfully prepared, which has wide application prospects.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
基金項(xiàng)目: 2017年福建省臨床重點(diǎn)專科建設(shè)項(xiàng)目1 通信作者,Email:[email protected]
更新日期/Last Update: 2020-07-04