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[1]章德昆 陳婷婷 陳 魁 王力群 龔 超 李 嵩 夏垚坤 陳敬華.一種用于microRNA-21濃度檢測(cè)的熒光傳感方法的建立[J].福建醫(yī)藥雜志,2020,42(01):132-134.
　ZHANG Dekun,CHEN Tingting,CHEN Kui,et al.Establishment of a fluorescence sensing method for microRNA-21 concentration detection[J].FUJIAN MEDICAL JOURNAL,2020,42(01):132-134.
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一種用于microRNA-21濃度檢測(cè)的熒光傳感方法的建立()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期數(shù):
2020年01期
頁(yè)碼:
132-134
欄目:
基礎(chǔ)研究
出版日期:
2020-03-30

文章信息/Info

Title:
Establishment of a fluorescence sensing method for microRNA-21 concentration detection
文章編號(hào):
1002-2600(2020)01-0132-03
作者:
章德昆1 陳婷婷2 陳 魁 王力群 龔 超 李 嵩 夏垚坤2 陳敬華2
福建醫(yī)科大學(xué)附屬福州市第一醫(yī)院(福州 350009)
Author(s):
ZHANG Dekun;  CHEN Tingting;  CHEN Kui;  WANG Liqun;  GONG Chao;  LI Song;  XIA Yaokun;  CHEN Jinghua.
The Affiliated Fuzhou Municipal First Hospital of Fujian Medical University, Fuzhou,Fujian 350009, China
關(guān)鍵詞:
聚多巴胺納米粒子;  miRNA-21;  DNA探針;  熒光檢測(cè)
Keywords:
polydopamine nanoparticles;  miRNA-21;  DNA probe;  fluorescence detection
分類(lèi)號(hào):
R917
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 建立一種用于microRNA-21(miRNA-21)濃度檢測(cè)的,基于聚多巴胺納米粒子(PDANs)的熒光傳感方法。方法 Ce6標(biāo)記的捕獲探針(Ce6-DNA)在Ca2+的介導(dǎo)下吸附在PDANs表面,熒光被猝滅。miRNA-21和Ce6-DNA雜交后,使其從PDANs表面解吸附,熒光恢復(fù)。利用熒光分光光度計(jì)分析靶標(biāo)和Ce6-DNA反應(yīng)前后熒光強(qiáng)度的變化,實(shí)現(xiàn)對(duì)miRNA-21的檢測(cè)。結(jié)果 在5.0～200 nM范圍內(nèi),670 nm處的熒光強(qiáng)度和miRNA-21的濃度呈良好的線性關(guān)系,檢測(cè)限為1.80 nM。結(jié)論 基于Ce6-DNA功能化的PDANs可為miRNA-21的定量檢測(cè)提供一種簡(jiǎn)單、準(zhǔn)確、成本低、分析速度快的新方法。
Abstract:
Objective To develop a fluorescent method based on polydopamine nanoparticles(PDANs)for microRNA-21(miRNA-21)concentration detection. Methods Ce6-labeled capture probe(Ce6-DNA)was absorbed on the surface of PDANs through Ca2+ mediation, which resulted in the fluorescence of Ce6 being quenched. However, the Ce6-DNA was desorbed from PDANs when it hybridized with miRNA-21, which led to the recovery of fluorescence. This method could detect miRNA-21 quantitatively through measuring the change of fluorescence intensity using fluorescent spectrophotometer. Results There was a good linear relationship between the fluorescence intensity at 670 nm and miRNA-21 concentration from 5.0 to 200 nM. The limit of detection was 1.80 nM. Conclusion The Ce6-DNA functionalized PDANs can provide a simple, accurate, low-cost, and rapid novel method for miRNA-21 detection.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金(2017J01226)1 Email:[email protected]; 2 福建醫(yī)科大學(xué)藥學(xué)院
更新日期/Last Update: 2020-03-30