80年代土耳其译制电影,80年代外国电影|80年代外国电影有哪些_80年代外国经典电影在线播放地址是多少？

[1]許揚(yáng)梅 龔福生 劉沁穎 劉施佳 黃麗潔 鄭秋紅.MiR-194過表達(dá)和抑制表達(dá)對肝癌細(xì)胞株Hep-3b中側(cè)群細(xì)胞增殖的影響[J].福建醫(yī)藥雜志,2019,41(06):141-144.
　XU Yangmei,GONG Fusheng,LIU Qinying,et al.Influence of miR-194 up and down-expression on liver cancer stem cells[J].FUJIAN MEDICAL JOURNAL,2019,41(06):141-144.
點(diǎn)擊復(fù)制

MiR-194過表達(dá)和抑制表達(dá)對肝癌細(xì)胞株Hep-3b中側(cè)群細(xì)胞增殖的影響()
分享到：

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期數(shù):
2019年06期
頁碼:
141-144
欄目:
基礎(chǔ)研究
出版日期:
2019-12-25

文章信息/Info

Title:
Influence of miR-194 up and down-expression on liver cancer stem cells
文章編號:
1002-2600(2019)06-0141-05
作者:
許揚(yáng)梅 龔福生 劉沁穎 劉施佳 黃麗潔 鄭秋紅1
福建醫(yī)科大學(xué)附屬腫瘤醫(yī)院 福建省腫瘤醫(yī)院 福建省腫瘤生物治療重點(diǎn)實(shí)驗(yàn)室(福州 350014)
Author(s):
XU Yangmei;  GONG Fusheng;  LIU Qinying;  LIU Shijia;  HUANG Lijie;  ZHENG Qiuhong.
Fujian Provincial Key Laboratory of Tumor Biotherapy,Fujian Medical University Cancer Hospital& Fujian Cancer Hospital,Fuzhou, Fujian 350014, China
關(guān)鍵詞:
微小RNA;  慢病毒載體;  肝癌;  干細(xì)胞
Keywords:
MicroRNA;  lentivirus vector;  liver cancer;  stem cell
分類號:
R735.7
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 研究MicroRNA-194(miR-194)過表達(dá)和抑制表達(dá)對細(xì)胞株Hep-3b中側(cè)群細(xì)胞增殖功能影響。方法 構(gòu)建miR-194過表達(dá)和抑制表達(dá)慢病毒并感染Hep-3b細(xì)胞。流式細(xì)胞儀鑒定各感染組SP側(cè)群比例,QPCR檢測各組細(xì)胞miR-194-5p表達(dá),CCK- 8法檢測各組細(xì)胞增殖能力,軟瓊脂克隆形成實(shí)驗(yàn)檢測細(xì)胞克隆形成能力。結(jié)果 流式細(xì)胞儀鑒定發(fā)現(xiàn)miR-194過表達(dá)Hep-3b組SP細(xì)胞含量與miR-194抑制表達(dá)Hep-3b組差異具有統(tǒng)計(jì)學(xué)意義(LSD-t=3.550,P=0.012)。QPCR檢測顯示miR-194過表達(dá)Hep-3b組miR-194-5p水平高于miR-194抑制表達(dá)Hep-3b組(LSD-t=4.680,P=0.007)。CCK- 8實(shí)驗(yàn)表明miR-194過表達(dá)Hep-3b組和miR-194抑制表達(dá)Hep-3b組細(xì)胞增殖能力差異有統(tǒng)計(jì)學(xué)意義(LSD-t=5.621,P=0.002)。軟瓊脂克隆形成實(shí)驗(yàn)顯示miR-194過表達(dá)Hep-3b組比miR-194抑制表達(dá)Hep-3b組形成細(xì)胞克隆少(LSD-t=5.849,P=0.001)。結(jié)論 成功構(gòu)建了miR-194過表達(dá)和抑制細(xì)胞株,miR-194抑制組顯示明顯的干細(xì)胞特征,其含有更多SP細(xì)胞,具有更強(qiáng)的細(xì)胞增殖能力和自我更新能力。
Abstract:
Objective To study the influence of miR-194 up and down-expression Hep-3b cell lines on the proliferation ability of SP cells.Methods Hep-3b was used to construct miR-194 up and down-expression cell lines via lentivirus infection.Flow cytometry was applied to identify SP cells.QPCR assay was adopted to test the expression of miR-194-5p in those cell lines.CCK-8 assay was conducted to study the proliferation ability of cells and soft agar colony formation assay was conducted to test colony-forming ability.Results Each infected cell line maintained good growth.Flow cytometry analysis showed that the SP percentage were significantly different between miR-194 up and down-regulation Hep-3b groups(LSD-t=3.550,P=0.012).QPCR assay of miR-194-5p showed statistic difference between miR-194 up and down-regulation Hep-3b groups(LSD-t=4.680,P=0.007).CCK-8 assay showed different growth ability between miR-194 up and down-regulation Hep-3b groups(LSD-t=5.621,P=0.002).Soft agar colony formation assay results less colonies formed in miR-194 up-regulation Hep-3b group than miR-194 down-regulation Hep-3b group(LSD-t=5.849,P=0.001).Conclusion miR-194 up-regulation Hep-3b group than miR-194 down-regulation Hep-3b group were successfully constructed.miR-194 down-regulation Hep-3b group contains more SP cells, possess stronger growth ability and self-renewal ability, showing stronger proliferation and self-renewal ability.

參考文獻(xiàn)/References:

[1] Yang F, Xiao Z, Zhang S.Knock down of miR-194-5p inhibits cell proliferation, migration and invasion in breast cancer by regulating the Wnt/β-catenin signaling pathway[J].Int J Mol Med, 2018, 42(6):3355-3363.
[2] Nofech-Mozes R, Khella H W, Scorilas A, et al.MicroRNA-194 is a marker for good prognosis in clear cell renal cell carcinoma[J].Cancer Med, 2016, 5(4):656-664.
[3] Li C F, Li Y C, Wang Y,et al.The effect of xLncRNA H19/miR-194-5p axis on the epithelial-mesenchymal transition of colorectal adenocarcinoma[J]. Cell Physiol Biochem, 2018, 50(1):196-213.
[4] Gao S, Zhao Z, Wu R, et al.MicroRNA-194 regulates cell viability and apoptosis by targeting CDH2 in prostatic cancer[J].Onco Targets Ther, 2018, 11(14):4837-4844.
[5] Zhao X, Hou Y, Tuo Z, et al.Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer[J].Exp Ther Med, 2018, 15(5):4179-4184.
[6] Zhao Y, Li F, Zhang X, et al.MicroRNA-194 acts as a prognostic marker and inhibits proliferation in hepatocellular carcinoma by targeting MAP4K4[J].Int J Clin Exp Pathol, 2015, 8(10):12446-12454.
[7] 滕浩,薛一雪,王萍,等.miR-194對人腦膠質(zhì)瘤U87細(xì)胞惡性生物學(xué)行為的影響[J]. 中國醫(yī)科大學(xué)學(xué)報(bào), 2018, 47(8):673-677.
[8] 王永晶,李娜.MiR-194在葡萄膜黑色素瘤細(xì)胞系中的表達(dá)及對細(xì)胞增殖、凋亡、遷移和侵襲的影響[J]. 眼科新進(jìn)展,2018,38(11):1033-1036.
[9] Gao X, Zhao P, Hu J, et al.MicroRNA-194 protects against chronic hepatitis B-related liver damage by promoting hepatocyte growth via ACVR2B[J]. J Cell Mol Med, 2018, 22(9): 4534-4544.
[10] Mahkamova K, Latar N M, Aspinall S, et al.Side population cells in anaplastic thyroid cancer and normal thyroid[J]. Exp Cell Res,2019, 374(1):104-113.
[11] Hotfilder M, Mallela N, Seggewi J, et al.Defining a characteristic gene expression set responsible for cancer stem cell-like features in a sub-population of ewing sarcoma cells CADO-ES1[J]. Int J Mol Sci, 2018, 19(12): E3908.
[12] Xu Y, Xie Y, Wang X, et al.Identification of cancer stem cells from hepatocellular carcinoma cell lines and their related microRNAs[J]. Oncol Rep, 2013,30(5): 2056-2062.
[13] Huang K, Zhu Y, Ma Y, et al.BMI1 enables interspecies chimerism with human pluripotent stem cells[J]. Nat Commun, 2018, 9(1):4649.
[14] Zhang X, Wei C, Li J,et al.MicroRNA-194 represses glioma cell epithelialtomesenchymal transition by targeting Bmi1[J]. Oncol Rep, 2017, 37(3):1593-1600.
[15] 張楊,楊琰,曹鈞.微小RNA-194對肝癌細(xì)胞增殖和侵襲的影響[J]. 中華實(shí)驗(yàn)外科雜志, 2018, 35(5): 842-844.

相似文獻(xiàn)/References:

[1]周子杰,張家豪,姜吉霖,等.miR-586通過SFRP1調(diào)控骨肉瘤細(xì)胞增殖、遷移、侵襲的機(jī)制研究[J].福建醫(yī)藥雜志,2024,46(01):115.[doi:10.20148/j.fmj.2024.01.032]

更新日期/Last Update: 2019-12-25