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[1]徐 楊 林飛躍 尹曉明.兩種體外培養(yǎng)體系對人骨髓間充質(zhì)干細(xì)胞原代培養(yǎng)、增殖和成骨分化的影響比較[J].福建醫(yī)藥雜志,2019,41(06):136-140.
 XU Yang,LIN Feiyue,YIN Xiaoming..Comparison of effect in two vitro culture systems in supporting human bone marrow mesenchymal stem cells for primary culture,propagation and osteogenic differentiation[J].FUJIAN MEDICAL JOURNAL,2019,41(06):136-140.
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兩種體外培養(yǎng)體系對人骨髓間充質(zhì)干細(xì)胞原代培養(yǎng)、增殖和成骨分化的影響比較()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期數(shù):
2019年06期
頁碼:
136-140
欄目:
基礎(chǔ)研究
出版日期:
2019-12-25

文章信息/Info

Title:
Comparison of effect in two vitro culture systems in supporting human bone marrow mesenchymal stem cells for primary culture,propagation and osteogenic differentiation
文章編號:
1002-2600(2019)06-0136-06
作者:
徐 楊 林飛躍 尹曉明
福建醫(yī)科大學(xué)省立臨床醫(yī)學(xué)院 福建省立醫(yī)院骨一科(福州 350001)
Author(s):
XU Yang LIN Feiyue YIN Xiaoming.
Department of Orthopedic Surgery,Fujian Provincial Hospital,Provincial Clinical Medical College of Fujian Medical University,Fuzhou, Fujian 350001, China
關(guān)鍵詞:
人血小板裂解液 無血清培養(yǎng) 骨髓間充質(zhì)干細(xì)胞 原代培養(yǎng) 增殖 成骨分化
Keywords:
human platelet lysate serum-free bone marrow mesenchymal stem cells primary culture propagation osteogenic differentiation
分類號:
R329.2+8
文獻(xiàn)標(biāo)志碼:
A
摘要:
目的 研究使用混合血小板裂解液(pHPL)和StemPro○R MSC無血清培養(yǎng)體系對人骨髓間充質(zhì)干細(xì)胞原代培養(yǎng)、增殖和成骨分化的影響。方法 分別使用異體濃縮血小板制備的混合血小板裂解液pHPL和StemPro○R MSC無血清培養(yǎng)體系原代培養(yǎng)人骨髓間充質(zhì)干細(xì)胞,以經(jīng)典的胎牛血清培養(yǎng)方案做對照,比較3組中的細(xì)胞形態(tài)、表面標(biāo)志物、細(xì)胞骨架、細(xì)胞周期和增殖率,并在加入經(jīng)典成骨誘導(dǎo)液后檢測3組中堿性磷酸酶含量、成骨相關(guān)基因含量和礦化結(jié)節(jié)形成,比較其成骨分化能力。結(jié)果 pHPL、StemPro○R MSC和FBS培養(yǎng)組均能較好支持人骨髓間充質(zhì)干細(xì)胞的原代培養(yǎng),3組中細(xì)胞形態(tài)、表面標(biāo)志物、細(xì)胞骨架、細(xì)胞周期均無明顯差異,pHPL和StemPro○R MSC組中細(xì)胞增殖率無明顯差異,但均明顯較FBS組高。在進(jìn)行成骨誘導(dǎo)后,pHPL組中堿性磷酸酶含量、成骨相關(guān)基因含量均較StemPro○R MSC組和FBS組高,3組中均能見到成熟的礦化結(jié)節(jié)形成。結(jié)論 pHPL和StemPro○R MSC均能夠很好地支持人骨髓間充質(zhì)干細(xì)胞的原代培養(yǎng),并且促進(jìn)細(xì)胞增殖的能力均較FBS為高,而且pHPL在促進(jìn)其成骨分化方面較StemPro○R MSC和FBS更強(qiáng)。
Abstract:
Objective To investigate effect of pooled human platelet lysate(pHPL)and StemPro○R MSC serum-free media in primary culture,propagation and osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMMSCs).Methods pHPL and StemPro○R MSC were used for primary culture of hBMMSCs,comparing with FBS method.Cell morphology,surface marker expression,cytoskeleton,cell cycle and cell proliferation rate were compared among the three groups.The content of alkaline phosphatase, the content of osteogenic related genes and the formation of mineralized nodule were detected after adding classical osteogenic induction solution, to compare their osteogenic differentiation ability among the three groups.Results Both pHPL and StemPro○R MSC could support primary culture of hBMMSCs as well as FBS.There were no differences between the three groups in cell morphology,surface marker expression,cytoskeleton and cell cycle.Cell proliferation rate wasnt significantly higher in pHPL group and StemPro○R MSC group,but it was significantly higher in both pHPL group and StemPro○R MSC group than FBS group.The content of alkaline phosphatase and the content of osteogenic related genes were significantly higher in pHPL group than both StemPro○R MSCgroupand FBS group.The formation of mineralized nodule could be seen in all three groups.Conclusion PHPL and StemPro○R MSC can well support the primary culture of human bone marrow mesenchymal stem cells,the ability to promote cell proliferation is higher than that of FBS,and pHPL is stronger in promoting osteogenic differentiation than StemPro○R MSC and FBS.

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備注/Memo

備注/Memo:
1 通信作者,副主任醫(yī)師,Email:[email protected]; 2 福建醫(yī)科大學(xué)附屬協(xié)和醫(yī)院急診內(nèi)科; 3 福建醫(yī)科大學(xué)神經(jīng)生物學(xué)中心
更新日期/Last Update: 2019-12-25