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[1]伍曉紅 王啟松 駱 凱 陳世煒.骨膜源性細(xì)胞和骨髓間充質(zhì)干細(xì)胞直接共培養(yǎng)的實驗研究[J].福建醫(yī)藥雜志,2019,41(04):118-121.
 WU Xiaohong,WANG Qisong,LUO Kai,et al.Experimental study on direct co-culture of periosteum-derived cells and bone marrow mesenchymal stem cells[J].FUJIAN MEDICAL JOURNAL,2019,41(04):118-121.
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骨膜源性細(xì)胞和骨髓間充質(zhì)干細(xì)胞直接共培養(yǎng)的實驗研究()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期數(shù):
2019年04期
頁碼:
118-121
欄目:
基礎(chǔ)研究
出版日期:
2019-08-15

文章信息/Info

Title:
Experimental study on direct co-culture of periosteum-derived cells and bone marrow mesenchymal stem cells
文章編號:
1002-2600(2019)04-0118-04
作者:
伍曉紅 王啟松1 駱 凱 陳世 煒2
福建醫(yī)科大學(xué)附屬口腔醫(yī)院牙周科(福州 350002)
Author(s):
WU Xiaohong WANG Qisong LUO Kai CHEN Shiwei
Department of Periodontology, the Affiliated Stomatological Hospital,Fujian Medical University,Fuzhou, Fujian 350002,China
關(guān)鍵詞:
骨膜細(xì)胞 骨髓基質(zhì)細(xì)胞 共培養(yǎng) 成骨分化 組織工程
Keywords:
periosteum bone marrow mesenchymal stem cells coculture techniques tissue engineering
分類號:
R336
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 探討骨膜源性細(xì)胞(PDCs)和骨髓間充質(zhì)干細(xì)胞(BMSCs)不同比例下直接共培 養(yǎng)對細(xì)胞的增殖及成骨分化的影響,優(yōu)選細(xì)胞直接共培養(yǎng)的適宜比例。方法 取PDCs和BMSCs, 在保證每組總細(xì)胞數(shù)一致的情況下,分別以PDCs和 BMSCs按2:1、1:1、1:2、1:0、0:1比例混 合培養(yǎng),通過細(xì)胞增殖能力,堿性磷酸酶活性(alkaline phosphatase activity, ALP)和礦化 結(jié)節(jié)形成的測定,來觀察PDCs和BMSCs不同比例共培養(yǎng)下細(xì)胞增殖和成骨分化能力。結(jié)果 PDCs和BMSCs直接共培養(yǎng)(2:1、1:1、1:2)的細(xì)胞增殖能力高于單獨PDCs組(1:0組)和單獨 BMSCs組(0:1組),但差異無統(tǒng)計學(xué)意義(P>0.05),共培養(yǎng)組細(xì)胞ALP表達(dá)較對照組 高(P<0.05),2:1組ALP表達(dá)最高(P<0.05),同時共培養(yǎng)組礦化結(jié)節(jié)形成 較多,1:0組和0:1組礦化結(jié)節(jié)形成較疏散。結(jié)論 PDCs和BMSCs直接共培養(yǎng)有利于細(xì)胞的增殖 能力和成骨能力,以2:1比例直接共培養(yǎng)時最佳,適宜進(jìn)一步的組織工程研究。
Abstract:
Objective To investigate the effects of direct co-culture ratio on the proliferation and osteogenic differentiation of PDCs and BMSCs, and to optimize the co-culture ratio.Methods PDCs and BMSCs were cultured at the ratio of 2:1, 1:1,1:2,1:0,0:1 respectively, and the total number of cells in each group was the same.The proliferation and osteogenic differentiation of PDCs and BMSCs co-cultured were observed by cell proliferation, alkaline phosphatase activity(ALP), and mineralized nodules formation.Results The cell proliferation of the co-culture group were higher than that of 1:0 and 0:1 groups, but there was no statistical differences between all groups(P>0.05).The ALP expression level of the co-culture group(2:1,1:1 and 1:2 ratio)especially in 2:1 ratio expressed higher than that of control groups(1:0 and 0:1 ratio) (P<0.05), and the mineralized nodules in co-culture groups were formed more than that in the control groups(1:0 and 0:1 ratio).Conclusion When PDCs and BMSCs was directly co-cultured at 2:1 ratio, the total cell proliferation and osteogenic ability are stronger than other groups, which are suitable for further tissue engineering research.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
基金項目:2015年福建省中青年教師教育科研項目(JA15199) 1 福建醫(yī)科大學(xué)口腔醫(yī)學(xué)重點實驗室; 2 通信作者
更新日期/Last Update: 2019-08-15