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[1]李淵根 鄭興中 黃榮桂.熊果酸對(duì)阿霉素腎病小鼠腎臟病變的影響[J].福建醫(yī)藥雜志,2018,40(06):123-125.
　LI Yuangen,ZHENG Xingzhong,HUANG Ronggui..Intervention effect of ursolic acid on adriamycin-induced nephropathy in mice[J].FUJIAN MEDICAL JOURNAL,2018,40(06):123-125.
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熊果酸對(duì)阿霉素腎病小鼠腎臟病變的影響()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
40
期數(shù):
2018年06期
頁碼:
123-125
欄目:
基礎(chǔ)研究
出版日期:
2018-11-29

文章信息/Info

Title:
Intervention effect of ursolic acid on adriamycin-induced nephropathy in mice
文章編號(hào):
1002-2600(2018)06-0123-04
作者:
李淵根 鄭興中1 黃榮桂2
福建醫(yī)科大學(xué)附屬第二醫(yī)院腎臟內(nèi)科(泉州 362000)
Author(s):
LI Yuangen;  ZHENG Xingzhong;  HUANG Ronggui.
Department of Nephrology,the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
關(guān)鍵詞:
熊果酸;  阿霉素腎病;  TGF-β1;  腎小管間質(zhì)纖維化
Keywords:
ursolic acid;  adriamycin-induced nephropathy;  TGF-β1;  renal tubulointerstitial fibrosis
分類號(hào):
R96
文獻(xiàn)標(biāo)志碼:
B
摘要:
目的 研究熊果酸(ursolic acid,UA)對(duì)小鼠阿霉素腎病(adriamycin-induced nephropathy,ADN)的影響,探討其可能的機(jī)制。方法 40只Balb/c小鼠,隨機(jī)分為正常對(duì)照組(Normal組),阿霉素腎病組(ADN組),低劑量熊果酸組(LU+ADN組),高劑量熊果酸組(HU+ADN組)。單次注射阿霉素創(chuàng)建阿霉素腎病模型,以熊果酸為干預(yù)因素,測定尿蛋白總量,蘇木素-伊紅(HE)染色觀察腎臟病變情況,免疫組織化學(xué)法檢測TGF-β1在腎小管間質(zhì)中的表達(dá)。結(jié)果 1)小鼠24 h尿蛋白總量:ADN組小鼠較Normal組顯著升高(P<0.05),HU+ADN組較ADN組顯著下降(P<0.05)。2)HE染色:ADN組出現(xiàn)顯著的腎小管上皮細(xì)胞腫脹,管腔擴(kuò)張等病變。與ADN組相比,HU+ADN組腎小管間質(zhì)病變較輕。3)免疫組化染色半定量分析:①與Normal組比,ADN組腎間質(zhì)表達(dá)TGF-β1顯著增加,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。②與ADN組比,LU+ADN組TGF-β1表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P>0.05); HU+ADN組小鼠TGF-β1表達(dá)顯著下降,兩組差異具有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 高劑量熊果酸(50 mg/kg)可以減少阿霉素腎病小鼠的尿蛋白,抑制阿霉素腎病小鼠腎小管間質(zhì)TGF-β1蛋白的表達(dá),發(fā)揮腎臟保護(hù)作用。
Abstract:
Objective To investigate the effect and mechanism of ursolic acid on adriamycin-induced nephropathy in mice. Methods Forty Balb/c mice were randomly divided into control group(Normal group),ADN group,low dose of UA group(LU+ADN group),high dose of UA group(HU+ADN group). The ADN model was established by treating with a single tail vein injection of ADR, UA was regarded as the interference factor. The Normal group received a single tail vein injection of saline only. Twenty four-hour urine was collected by urine protein tests. HE staining was used to obverse the tubulointerstitial lesions, and immunohistochemical staining detected the expression of TGF-β1 in tubulointerstitium. Results 1)24-hour urinary protein excretion:At day 7 after ADR injection, albuminuria was most evident in ADN group as compared with Normal group(P<0.05), and was attenuated in HU+ADN group as compared with ADN group(P<0.05). 2)HE staining: The renal tubulointerstitial lesions of ADN group mice were obvious, with swelling of tubular epithelial cells, tubular dilation obviously. The tubulointerstitial lesions of HU+ADN group mice were relatively relieved. 3)The semi-quantitative analysis of immunohistochemical staining: Compared with Normal group, the expression level of TGF-β1 in renal tissue of ADN group was significantly increased(P<0.05). Compared with ADN group, difference of the expression level of TGF-β1 in renal tissue of LU+ADN group had no statistics significance(P>0.05). Compared with ADN group, the expression level of TGF-β1 in renal tissue of HU+ADN group was significantly reduced(P<0.05). Conclusion The high concentration of UA(50 mg/kg)has a protection effect on mice with adriamycin-induced nephropathy by reducing urinary protein excretion and inhibiting the TGF-β1 protein expression in tubulointerstitial.

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更新日期/Last Update: 2018-11-29