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[1]張紫冠 胡 森 陳文博 黃崢嶸 李衛(wèi)華.小G蛋白在心肌鈉通道蛋白轉運中的作用[J].福建醫(yī)藥雜志,2018,40(06):114-117.
 ZHANG Ziguan,HU Sen,CHEN Wenbo,et al.Role of small GTP-binding proteins in cardiac voltage-gated sodium channel transport[J].FUJIAN MEDICAL JOURNAL,2018,40(06):114-117.
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小G蛋白在心肌鈉通道蛋白轉運中的作用()
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《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

卷:
40
期數(shù):
2018年06期
頁碼:
114-117
欄目:
基礎研究
出版日期:
2018-11-29

文章信息/Info

Title:
Role of small GTP-binding proteins in cardiac voltage-gated sodium channel transport
文章編號:
1002-2600(2018)06-0114-05
作者:
張紫冠 胡 森 陳文博 黃崢嶸 李衛(wèi)華1
廈門大學附屬第一醫(yī)院心內(nèi)科 廈門大學醫(yī)學院心律失常與心臟電生理實驗室(廈門 361003)
Author(s):
ZHANG Ziguan HU Sen CHEN Wenbo HUANG Zhengrong LI Weihua.
Arrhythmia and Cardiac Electrophysiology Laboratory,Medical College of Xiamen University; Department of Cardiovascular,the First Affiliated Hospital of Xiamen University, Xiamen,Fujian 361003,China
關鍵詞:
小G蛋白 Sar1 ARF1 SCN5A 基因 Nav1.5 蛋白
Keywords:
small GTPases Sar1 ARF1 SCN5A gene Nav1.5
分類號:
R363.1+4
文獻標志碼:
A
摘要:
目的 研究小G蛋白Sar1和Arf1對鈉通道蛋白(Nav1.5)轉運的調控作用。方法 以HEK293細胞作為宿主細胞,將人SCN5A基因轉染到HEK293細胞中,通過G418篩選穩(wěn)定表達SCN5A基因的細胞系; 為了檢測小G蛋白對Nav1.5的調節(jié)效應,野生型或突變型的小G蛋白Sar1和ARF1分別瞬轉到HEK293-Nav1.5細胞。利用免疫印跡對目標蛋白進行定量,應用免疫熒光對細胞內(nèi)的目標蛋白進行定位。結果 過表達突變的Sar1減少了細胞膜上Nav1.5的表達和抑制了Nav1.5轉運到細胞膜上。另外,過表達ARF1對于Nav1.5在細胞的表達及定位無明顯影響。結論 小G蛋白Sar1可能參與調節(jié)Nav1.5在細胞內(nèi)的運輸。
Abstract:
Objective To investigate the role of small GTPases Sar1 and Arf1 in the transcription of Nav1.5 protein. Methods In this experiment,we used HEK 293 cells as the host cells to co-express Nav1.5 with the wild-type(WT)or mutant form of the small GTPases Sar1 and Arf1. Then in order to check the effect that small GTPase had on Nav1.5 regulation, two techniques were used to analyze the results. Western blot was performed to measure the expression level of Nav1.5, and Confocal microscopy was used to visualize the cellular localization of Nav1.5. Results We found that mutant forms of Sar1 decreased Nav1.5 expression level significantly and inhibited its localization at the cell membrane. On the other hand, Arf1 only showed small effect on the expression level and localization of Nav1.5. Conclusion This study provides evidences that Nav1.5 intracellular transport is regulated by small GTPases Sar1 in particular.

參考文獻/References:

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備注/Memo

備注/Memo:
基金項目:福建省自然科學基金資助項目(2015J01563) 1 通信作者,Email:[email protected]
更新日期/Last Update: 2018-11-29