香加皮杠柳苷對小鼠骨髓瘤細(xì)胞增殖、凋亡及移植成瘤的影響及作用機(jī)制-《福建醫(yī)藥雜志》

《福建醫(yī)藥雜志》[ISSN:1002-2600/CN:35-1071/R]

Title:: Effects and mechanism of action of periplocin from cortex periplocae on proliferation,apoptosis and transplantation of myeloma cells in mice

Keywords:: periplocin from cortex periplocae (CPP); myeloma cells; proliferation; apoptosis

摘要:: 目的研究香加皮杠柳苷(CPP)對小鼠骨髓瘤細(xì)胞增殖、凋亡及移植成瘤的影響及作用機(jī)制.方法通過用不同濃度的香加皮杠柳苷對SP2/0小鼠骨髓瘤細(xì)胞及小鼠體內(nèi)移植瘤進(jìn)行干預(yù)處理,MTT法檢測CPP處理的SP2/0小鼠骨髓瘤細(xì)胞的增殖抑制率,流式細(xì)胞儀檢測小鼠骨髓瘤細(xì)胞的凋亡率,Westernblot檢測小鼠骨髓瘤細(xì)胞及移植瘤中IL-6和DKK1蛋白的表達(dá)水平.結(jié)果不同濃度CPP能抑制SP2/0小鼠骨髓瘤細(xì)胞增殖,加速SP2/0小鼠骨髓瘤細(xì)胞凋亡,在裸鼠體內(nèi),不同濃度CPP對抑制骨髓瘤移植成瘤,同時降低骨髓瘤細(xì)胞和移植瘤中IL-6和DDK1蛋白的表達(dá)水平.結(jié)論香加皮杠柳苷能夠通過抑制IL-6和DDK1表達(dá),阻斷Wnt信號來達(dá)到治療小鼠骨髓瘤的作用,有望成為治療多發(fā)性骨髓瘤及骨髓瘤骨病的新藥物.

Abstract:: Objective To investigate the effects and mechanism of action of the periplocin from cortex periplocae(CPP) on proliferation,apoptosis and transplantation of myeloma cells in mice.Methods The different concentrations of the periplocin from cortex periplocae were used on SP2/0 myeloma cells and mice transplanted tumor.The proliferation inhibition rate of SP2/0 myeloma cells was determined by MTT method,the cells apoptosis rate of SP2/0 myeloma cells was determined by flow cytometer,the expression level of IL-6 and DKK1 in SP2/0 myeloma cells and mice transplanted tumor were analyzed by Western blot.Results The proliferation of SP2/0 myeloma cell was inhibited and the apoptosis of SP2/0 myeloma cell was accelerated by different concentrations of CPP,the myeloma transplanted tumor was inhibited and the expression level of IL-6 and DDK1 were reduced by different concentrations of CPP myeloma cells in SP2/0 myeloma cell and transplanted tumor.Conclusion CPP could inhibit IL-6 and DDK1 expression,and block Wnt signal to achieve the effect of treatment of mice myeloma.It is expected to be a new drug for the treatment of multiple myeloma(MM) and myeloma bone disease(MBD).

更新日期/Last Update: 1900-01-01